Manuscript Information Authors

[1]	Imarenezor Edobor Peter Kenneth, Tropical Diseases Unit, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University, Wukari, Nigeria.
[2]	Iyamu Mercy Itohan, Department of Microbiology, Faculty of Life Sciences, Ambrose Alli University, Ekpoma, Nigeria.
[3]	Brown Samuel Tamunoiyowuna Cockeye, Tropical Diseases Unit, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University, Wukari, Nigeria.

Abstract

This study was aimed at isolating bacteria associated with urinary tract infection (UTIs) in pregnant and non-pregnant women and their anti-biogram. Forty (40) samples were collected from both pregnant and non-pregnant women. The pregnant women samples were from antenatal clinic at General Hospital, Ewohimi, Edo State while the non-pregnant women were from apparently healthy students in Ewohimi. Identification of isolates was done with standard microbiological methods. Antibiotic susceptibility test was by disc diffusion method. Bacteria isolated from pregnant women were Escherichia coli 4(50%), Staphylococcus aureus 3(37.50%), and Enterobacter aerogenes 1(12.50%). Escherichia coli 3(50%), Klebsiella pneumoniae 2(33.33%) and Stahylococcus. saprophyticus 1(12.50%) were isolated from non-pregnant women. Antibiotic susceptibility testing of isolates showed Escherichia coli isolates was 100% sensitive to Ceftazidime, Gentamycin, Ofloxacin, and Augumentin, 57.14% sensitive to Nalidixic acid and Nitrofurantoin, and 71.43% sensitive to Ceftriaxone. It was 100% resistant to Tetracycline, Cotrimoxazole and Amoxicillin, 42.86% resistant to Nalidixic acid and Nitrofurantion and 28.57% resistant to Ceftriaxone. Staphylococcus aureus isolated from pregnant women was 100% sensitive to Ceftazidime, Gentamycin and Augumentin, 66.67% sensitive to Cloxacillin, and Chloramphenicol, 33.33% sensitive to Ofloxacin, Tetracycline, Cotrimoxazole, Amoxcillin, Streptomycin and Erythromycin. It was however, 100% resistant to Ceftriaxone. Klebsiella Pneumoniae isolated from non-pregnant women was 100% sensitive to Ceftriazone, Nalidixic Acid, Gentamycin, Augumentin, Tetracycline, and Ofloxacin, but 100% resistant to Cotrimoxazole and Amoxicillin. Enterobacter aerogenes and Stahylococcus saprophticus isolates from pregnant and non-pregnant women were 100% sensitive to Ceftazidime, Nalidixic acid, Gentamycin, Ofloxacin, Tetracycline, Ofloxacin, Cloxacillin, Streptomycin, Chloramphenicol and Augumentin, 100% resistant to Cotrimoxazole, Amoxicillin, Ceftazidime, Gentamycin, Cotrimoxazole and Erythromycin. Thus for all the bacterial isolates, Ceftazidime and Amoxicillin had the highest and lowest antimicrobial activities respectively. Results from this finding underscore the need for frequent urine examination for pregnant women and continuous maintenance of high standards of personal hygiene.

Keywords

Bacteria, UTI, Pregnant, Non-pregnant, Women, Antibiogram

Reference

[01]	Nicolle, L. E. (2008). Uncomplicated urinary tract infection in adults including uncomplicated pyelonephritis. Urology Clinic North America. 35 (1): 1-12; v.
[02]	Lin, K., and Fajardo. (2008). Screening for asymptomatic bacteriuria in adults, evidence for the U.S preventive services task force reaffirmation recommendation statement. Annals of Internal Medicine. 149 (1): 20-24.
[03]	Azzarone, G., Liewehr, S., and O’Connor, K. (2007). Cystitis Pediatrics in Review. 28 (12): 474-476.
[04]	Karl, K. (2008). Science is golden, P. 175, ISBN 978-0-7322-85364.
[05]	Rosenthal, K. S., Murray, P. R., and Kobayashi, G. S. (2009). The big picture Medical Microbiology, 6th Edition chapter 34, Pg 342, Mc Graw-Hill.
[06]	Ezechi, D. C., Dare, F. O and Fasubaa O. (1998). Sensitivity pattern of microbial infection of the urinary tract in pregnant women. Society of Gynecology and Obstetrics of Nigeria. 5: 62.
[07]	Lane, D. R., and Takhar, S. S. (2011). Diagnosis and management of Urinary tract infections and Pyelonephritis. Emergency Medicine Clinics of North America. 29 (3): 539-552.
[08]	Colgan, R., Nicolle, L. E., McGlone, A., and Hooton, T. M. (2006). Asymptomatic Bacteriuria in Adults. American family physician. 74 (6): 985-990.
[09]	Colgan, R., Williams, M., and Johnson, J. R. (2011). Diagnosis and treatment of acute pyelonephritis in women. American family physician. 84 (5): 519-526.
[10]	Johnson, J. R., and Stamm, W. E. (1989). Urinary tract infection in women; diagnosis and treatment. Annals of Internal Medicine. 111: 906-917.