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Production, Optimization and Pigment Production Potential of Serratia marcescens (GBB151) Isolated from the Industrial Effluent

World Journal of Biochemistry and Molecular Biology
Vol.3 , No. 1, Publication Date: May 9, 2018, Page: 6-15

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Authors

[1]	Cecilia Nireti Fakorede, Department of Biological Sciences, College of Natural and Applied Sciences Oduduwa University, Ipetumodu, Nigeria.
[2]	Olu Odeyemi, Department of Microbiology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
[3]	Gbolahan Babalola, Department of Microbiology, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.
[4]	Babamotemi Itakorode, Department of Biochemistry, Faculty of Science, Obafemi Awolowo University, Ile-Ife, Nigeria.

Abstract

This research was carried out to isolate pigment producing bacterium from industrial effluent. It also optimized and characterized the pigment produced by the bacterium. A total of twenty pigments producing bacteria were isolated based on different colours exhibited by the colonies on Nutrient agar and one was selected for further study based on its appreciable pigment production. The optimal conditions for pigment production were determined by varying the pH, temperature, nitrogen sources and carbon sources. Characterizations of the pigment were carried out using thin layer chromatography (TLC), FT-IR spectroscopy and Gas Chromatography-Mass Spectrometry (GCMS). The potential isolate was identified using 16s rRNA sequencing and confirmed as Serratia marcescens. The optimum pigment production was obtained at 30^th hour, temperature of 30°C and optimum pH of 7.0. The best nitrogen and carbon source for production were found to be peptone and maltose with pigment production of 0.668 mg/l and 0.764 mg/l respectively. TLC analyses of the extracted pigment revealed the presence of a reddish single spot. Also, FTIR analysis indicated the presence of O-H and alkene functional group in the pigment. (GC-MS) analysis identified the active components in the pigment as n – haxadenoic acid (20.90%), phenol 2, 4-bis (1, 1 – dimethyl) (17.83%), octadecenoic acid (9.81%) and cis – vaccenic acid (8.53%). The colour of the pigments was stable over a wide range of pH and the pigment also performed excellently as a dye. The obtained results showed that Serratia marcescens pigment could find application in industrial processes.

Keywords

Industrial Effluent, Serratia marcescens, Isolate, Pigment

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