World Journal of Biochemistry and Molecular Biology  
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Circulating Genotypes and Drug Resistance Profiles of Mycobacterium tuberculosis in Brazilian Counties Bordering Paraguay and Bolivia
World Journal of Biochemistry and Molecular Biology
Vol.3 , No. 2, Publication Date: May 16, 2018, Page: 55-65
1915 Views Since May 16, 2018, 343 Downloads Since May 16, 2018
 
 
Authors
 
[1]    

Marli Marques, School of Medicine, Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.

[2]    

Eunice Atsuko Totumi Cunha, Health Central Laboratory of Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.

[3]    

Adolfo C. Barreto Santos, Department of Biological Sciences, Universidade Estadual Paulista, Araraquara, São Paulo, Brazil.

[4]    

Clarice Queico Fujimura Leite, Department of Biological Sciences, Universidade Estadual Paulista, Araraquara, São Paulo, Brazil.

[5]    

Antonio Francisco de Souza Filho, School of Veterinary Medicine and Animal Science, Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.

[6]    

Sonia Maria Oliveira de Andrade, School of Medicine, Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, Brazil.

 
Abstract
 

Along the southern part of Brazil’s border with Paraguay and Bolivia, tuberculosis control is a challenging task. This scenario has prompted investigations to support the implementation of specific control measures for the region. This study examined spoligotyping of 60 isolates of M. tuberculosis from patients with pulmonary tuberculosis reported in the 2007-2010 period who resided in 12 Mato Grosso do Sul counties bordering Paraguay and Bolivia. Fifty-six strains with distinctive patterns and four orphan strains (6.7%) were identified. Family LAM predominated (30 cases; 50%), followed by families T (subfamily T1; 14 cases; 23.3%), Haarlem (8 cases; 13.3%), and families X (subfamily X1) and S (1 case, or 11.7%, each). Beijing genotypes were absent. A high rate of similarity of 75.1% (45) was found for 10 clusters, comprising subfamilies LAM9 (SIT42), T1 (SIT53), H1 (SIT47), LAM6 (SIT1610), LAM3 (SIT33), H3 (SIT50), and LAM5 (SIT1337) for both binational borders. Sensitivity testing for rifampicin, isoniazid, streptomycin, and ethambutol was performed in 80% (48) of isolates, with drug resistance detected in 35.4% (17). In the border areas investigated, pulmonary tuberculosis exhibited low genetic diversity and high drug resistance, calling for effective epidemiologic surveillance efforts and supplementary molecular studies.


Keywords
 

Mycobacterium tuberculosis, Genetic Lines, Spoligotyping, Resistance Patterns, Brazil–Paraguay–Bolivia Borders


Reference
 
[01]    

World Health Organization, Global tuberculosis report 2017, World Health Organization Geneva, Switzerland, 2017.

[02]    

Ministério da Saúde, Secretaria de Vigilância em Saúde. Boletim Epidemiológico: Tuberculose, vol. 48, no. 8, pp. 1-11, 2017.

[03]    

M. Marques, A. Ruffino-Netto, A. M. C. Marques, S. M. O. de Andrade, B. A. K. Silva and E. R. F. C., “Magnitude da tuberculose pulmonar na população fronteiriça de Mato Grosso do Sul (Brasil), Paraguai e Bolívia”, Cadernos de Saúde Pública, Rio de Janeiro, vol. 30, no. 12, pp. 2631-2642, 2014.

[04]    

J. U. Braga, B. H. Herrero and C. M. Cuellar, “Transmissão da tuberculose na tríplice fronteira entre Brasil, Paraguai e Argentina”, Cadernos de Saúde Pública, vol. 27, no. 7, pp. 1271-1280, 2011.

[05]    

R. A. Silva-Sobrinho, R. L P. Andrade, M. A. Z. Ponce et al., “Retardo no diagnóstico da tuberculose em município da tríplice fronteira Brasil, Paraguai e Argentina”, Revista Panamericana de Salud Publica, vol. 31, n. 6, p. 461-468, 2012.

[06]    

Mato Grosso do Sul. Mato Grosso do Sul sem fronteiras: características e interações territoriais: Brasil, Bolívia, Paraguai, Campo Grande, Mato Grosso do Sul, 2010.

[07]    

E. A. T. Cunha, “Caracterização molecular das cepas de Mycobacterium tuberculosis da população indígena e não-indígena de Mato Grosso do Sul. Campo Grande”, PhD Thesis, Universidade Federal de Mato Grosso do Sul, Campo Grande, Mato Grosso do Sul, 2011.

[08]    

C. Demay, B. Liens, T. Burguiere et al., “SITVITWEB-a publicly available international multimarker database for studying Mycobacterium tuberculosis genetic diversity and molecular epidemiology”, Infection, Genetics and Evolution, vol. 12, no. 4, pp. 755–766, 2012.

[09]    

P. F. Barnes and M. D. Cave, “Molecular Epidemiology of Tuberculosis”, New England Journal of Medicine, vol. 349, no. 12, pp. 1149-1156, 2003.

[10]    

H. O. F. Molhuizem, A. E. Bunschoeten, L. M. Schouls, and J. D. A. Van Embden, “ Rapid detection and simultaneous strain differentiation of Mycobacterium tuberculosis complex bacteria by spoligotyping”, Methods in Molecular Biology, vo. 101, no. 2, pp. 381-394, 1998.

[11]    

G. Canetti, “Present aspects of bacterial resistance in tuberculosis”, The American Review of Respiratory Disease, vol. 92, no. 5, pp. 687-703, 1965.

[12]    

J. Kamerbeek, L. Shouls, A. Kolk et al., “Simultaneous detection and stain differentiation of Mycobacterium tuberculosis for diagnosis and epidemiology”, Journal of Clinical Microbiology, vol. 35, no. 4, pp. 907-914, 1997.

[13]    

K. Brudey, J. R. Driscoll, L. Rigouts et al., “Mycobacterium tuberculosis complex genetic diversity: mining the fourth international Spoligotyping database (SpolDB4) for classification, population genetics and epidemiology”, BMC Microbiology, vol. 6, no. 23, pp. 6-23, 2006.

[14]    

Pan American Health Organization, Tuberculosis in the Americas: Regional Report 2012, Epidemiology, Control and Financing, Pan American Health Organization, Washington, United States of America, 2013.

[15]    

M. Marques, E. A. T. Cunha, M. S. N. Evangelista, et al., “Resistência às drogas antituberculose na fronteira do Brasil com Paraguai e Bolívia”, Rev Panam Salud Publica, vol. 41, 20 Abr 2017.

[16]    

M. Palaci, R. L. Peres, R. Maia et al., “Contribution of the Ogawa-Kudoh swab culture method to the diagnosis of pulmonary tuberculosis in Brazil”, International Journal Tuberculosis Lung Disease, vol. 17, no. 6, pp. 782–786, 2013.

[17]    

G. P. Kubica, P. P. Gontijo-Filho and T. Kim, “ Preservation of Mycobacteria at 70°C: persistence of key differential features”, Journal of Clinical Microbiology, vol. 6, no. 2, pp. 149-153, 1977.

[18]    

P. C. Basta, M. Marques, R. L. Oliveira, E. A. T. Cunha, A. P. C. Resendes and R. Souza-Santos, “Desigualdades sociais e tuberculose: análise segundo raça/cor, Mato Grosso do Sul”, Revista de Saúde Pública, vol. 47, no. 5, pp. 854-864, 2013.

[19]    

L. N. Machado, N. R. Marcondes, C. Q. F. Leite et al., “First baseline of circulating genotypic lineages of Mycobacterium tuberculosis in patients from the brazilian borders with Argentina and Paraguay”, PLoS One, vol. 9, article ID e107106, 2014.

[20]    

P. Meza, M. E. Balcells, C. Miranda, M. Cifuentes, A. Wozniak y P. García.. “Presencia del genotipo Beijing entre cepas del complejo Mycobacterium tuberculosis en dos centros de salud de la Región Metropolitana-Chile”, Rev. Chil. Infectol. vol. 31, no. 1, p. 21-27, 2014.

[21]    

V. Guernier, C. Sola, K. Brudey, J. F. Guégan, N. Rastogi, “Use of cluster-graphs from spoligotyping data to study genotype similarities and a comparison of three indices to quantify recent tuberculosis transmission among culture positive cases in French Guiana during a eight year period”, BMC Infectious Diseases, vol. 8, no. 46. pp. 1-12, 2008.

[22]    

H. M. Gomes, A. R. Elias, M. A. Oelemann et al., “Spoligotypes of Mycobacterium tuberculosis complex isolates from patients residents of 11 states of Brazil,” Infection Genetics and Evolution, vol. 12, no. 4, pp. 649-656, 2012.

[23]    

A. L. Barros, Gente Pantaneira: crônicas de sua história, 1st ed. Lacerda Editores, Rio de Janeiro, 1998, 251 pp.

[24]    

J. R. Marin, Hibridismo cultural na fronteira do Brasil com Paraguai e Bolívia, In Abdala- Júnior B, Scarpelli MF (eds.), Portos flutuantes: trânsitos ibero-afro- americanos, 1st ed. Atelie Editorial, São Paulo, pp. 325-342.

[25]    

N. Candia, B. Lopez, T. Zozio et al., “First insight into Mycobacterium tuberculosis genetic diversity in Paraguay,” BMC Microbiology, vol. 7, no. 75, pp. 75-85, 2007.

[26]    

V. Ritacco, B. López, M. Ambroggi et al., “HIV infection and geographically bound transmission of drug-resistant tuberculosis, Argentina”, Emergency Infectious Diseases, vol. 18, no. 11, pp. 1802-1810, 2012.

[27]    

C. M. Sequera, S. V. Delgado, M. W Araque et al., “Mycobacterium tuberculosis: spoligotypes in the Carabobo state, Venezuela”, Revista Chilena de Infectología, vol. 25, no. 5, pp. 362-267, 2008.

[28]    

T. Realpe, N. C. Nidia, R. C. Rozo et al., “Population structure among Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Colombia”, PLoS One, vol. 9, no. 4, pp. 1-7, 2014.

[29]    

C. L. Nogueira, “Diagnóstico e epidemiologia molecular de cepas de Mycobacterium tuberculosis no Estado de Santa Catarina”, PhD Thesis, Universidade Federal de Santa Catarina, Santa Catarina, 235 pp. 2012.

[30]    

A. C. Malaspina, H. R. Cavalcanti, Leite C. Q. F. et al., “Usefulness of Mycobacterium tuberculosis molecular typing in a tuberculosis low-endemic agro- industrial setting of Brazil”, Japanese Journal of Infection Disease. vol. 61, no. 3, pp. 231-233, 2008.

[31]    

P. I. Cafrune, L. G. Possuelo, A. W. Ribeiro et al., “Prospective study applying spoligotyping directly to DNA from sputum sample of patients of having tuberculosis”, Journal of Clinical Microbiology, vol. 55, no. pp. 895-900, 2009.

[32]    

E. N. Noguti, C. Q. F. Leite, A. C. Malaspina et al., “Genotyping of M. tuberculosis isolates from a low-endemic setting in northwestern state of Paraná in Southern Brazil”, Memórias do Instituto Oswaldo Cruz, vol. 105, no. 6, pp. 779-785, 2010.

[33]    

M. Monteserin, M. Camacho, L. Barrera, J. C. Palomino, V. RitaccV and A. Martin, “Genotypes of Mycobacterium tuberculosis in patients at risk of drug resistance in Bolivia”, Infection, Genetics and Evolution, vol. 17, pp. 195-201, 2013.

[34]    

V. Ritacco, B. López, P. I. Cafrune, et al., “Mycobacterium tuberculosis strains of the Beijing genotype are rarely observed in tuberculosis patients in South America”, Memórias do Instituto Oswaldo Cruz, vol. 103, no. 5, pp. 489-492, 2008.

[35]    

S. S. Miranda, W. A. S. Carvalho, P. N. Suffys et al., “Spoligotyping of clinical Mycobacterium tuberculosis isolates from the state of Minas Gerais, Brazil”, Memórias do Instituto Oswaldo Cruz, vol. 106, no. 3, pp. 267-273, 2011.

[36]    

S. David, D. R. Ribeiro, A. Antunes, C. Portugal, L. Sancho and J. G. de Sousa, “Contribution of spoligotyping to the characterization of the population structure of Mycobacterium tuberculosis isolates in Portugal”, Infection, Genetic and Evolution, vol. 7, no. 5, pp. 609-617, 2007.

[37]    

M. C. Martins, C. M. Giampaglia, R. S. Oliveira, et al., “Population structure and circulating genotypes of drug-sensitive and drug-resistant Mycobacterium tuberculosis clinical isolates in São Paulo state, Brazil”, Infection, Genetic and Evolution, vol. 14, Mar, pp. 39-45, 2013.

[38]    

L. Aristimuño, R. Armengol, A. Cebollada, et al., “Molecular characterisation of Mycobacterium tuberculosis isolates in the First National Survey of Anti-tuberculosis Drug Resistance from Venezuela”, BMC Microbiology, vol. 6, no. 90 pp. 1-12, 2006.

[39]    

K. Brudey, I. Filliol, S. Ferdinand et al., “Long-term population-based genotyping study of Mycobacterium tuberculosis complex isolates in the French Departments of the Americas”, Journal of Clinical Microbiology, vol. 44, pp. 183-191, 2006.

[40]    

European Union. European Concerted Action on New Generation Genetic Markers and Techniques for the Epidemiology and Con¬trol of Tuberculosis, “Beijing⁄W genotype Mycobacterium tuberculosis and drug resistance”, Emerging Infectious Diseases, vol. 12, no. 5, pp. 736-743, 2006.

[41]    

B. J. Marais, T. C. Victor, A. C. Hesseling et al., “ Beijing and Haarlem genotypes are overrepresented among children with drug-resistant tuberculosis in the Western Cape province of South Africa”, Journal of Clinical Microbiology, vol. 44, no. 10, pp. 3539-3543, 2006.

[42]    

P. Sheen, D. Couvin, L. Grandjean, et al., “Genetic Diversity of Mycobacterium tuberculosis in Peru and Exploration of Phylogenetic Associations with Drug Resistance”, PLoS ONE, vol. 8, no. 6, 2013.

[43]    

V. Ritacco, M. J. Iglesias, L. Ferrazoli, et al., “Conspicuous multidrugresistant Mycobacterium tuberculosis cluster strains do not trespass country borders in Latin America and Spain”, Infection, Genetic and Evolution, 12, pp. 711–717, 2012.

[44]    

R. I. Prim, M. A. Schörner, S. G. Senna, et al., “Molecular profiling of drug resistant isolates of Mycobacterium tuberculosis in the state of Santa Catarina, southern Brazil”, Memórias do Instituto Oswaldo Cruz, vol. 1, no. 6, pp. 1-6, 2015.

[45]    

F. P. C. Sacchi, M. B. Tatara, C. C. Lima et al., “Genetic Clustering of Tuberculosis in an Indigenous Community of Brazil”, The American Journal of Tropical Medicine and Hygiene, vol. 98, no. 2, pp. 372–375, 2018.

[46]    

F. P. C. Sacchi, R. M. Praça, M. B. Tatara et al. “Prisons as reservoir for community transmission of tuberculosis, Brazil,” Emerging Infectious Diseases, vol. 21, no. 3, p. 452-455, 2015





 
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