World Journal of Biochemistry and Molecular Biology  
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Quantification of Protein and Amino Acid Composition in Some Oilseeds
World Journal of Biochemistry and Molecular Biology
Vol.2 , No. 2, Publication Date: Aug. 25, 2017, Page: 8-11
519 Views Since August 25, 2017, 764 Downloads Since Aug. 25, 2017
 
 
Authors
 
[1]    

Arowora Kayode Adebisi, Department of Biochemistry, Faculty of Pure and Applied Science, Federal University Wukari, Wukari, Nigeria.

[2]    

Ezeonu Chukwuma Stephen, Department of Biochemistry, Faculty of Pure and Applied Science, Federal University Wukari, Wukari, Nigeria.

[3]    

Imo Chinedu, Department of Biochemistry, Faculty of Pure and Applied Science, Federal University Wukari, Wukari, Nigeria.

[4]    

Udeogu Emmanuel, Department of Biochemistry, Faculty of Pure and Applied Science, Federal University Wukari, Wukari, Nigeria.

 
Abstract
 

The study investigated the levels of protein, energy, and moisture content as well as amino acid composition in some oilseed samples, using High Performance Liquid Chromatographic (HPLC) Technique, while other analysis were carried out using the standard methods of Association of official analytical chemists. All the samples were procured from Wukari market, Taraba State. The mean moisture content determined for the samples was at safe levels at the range (8.76±0.0141 - 9.75±0.0000 %). The highest protein level obtained (40.006±0.0078 %) was in Glycine max while the least was observed in Citrullus vulgaris (20.449±0.0233 %). Amino acids had the following range in the samples analysed such as: Glycine max (2.065±0.007-9.115±0.0070 g/100g), Sesamum indicum (Sesame seeds) (1.5±0.0141 - 9.11±0.0141 g/100g), Arachis hypogaea ((1.645±0.0070-9.710 g/100g), and Citrullus vulgaris seed samples (1.050±0.0141-8.150±0.0141 g/100g). The study revealed that Glycine max (Soybean seeds) had the highest level of energy and protein composition among all samples analysed, while the lowest was Citrullus vulgaris (Melon seeds). Based on the findings of this study, it can be concluded that soybeans can be utilized as an ideal source of protein for formulation of infant foods for quick recovery of infants and children suffering from Protein Energy Malnutrition (PEM).


Keywords
 

Amino Acid, Groundnut, Melon, Oilseed, Protein, Soybean, Wukari


Reference
 
[01]    

Singh, U., Rao, Subrahmanuam, V. P. N. and Sexena, P. (1993). Cooking, chemical composition and protein quality of newly developed genotypes of pigeon pea (Canjanus cajan L). J. Sci. Food Agric., 61:395-400.

[02]    

FAO. (2002). United Nations Food and Agricultural Organisation. Protein sources for the animal feed industry. Animal production and health paper No 1. Accessed June 06, 2016.

[03]    

AOAC. (2000). Association of Official Analytical Chemists. 18th ed, Official Methods of Analysis. Washington D. C: pp. 18-62.

[04]    

Ojiako, O. A. & Igwe, C. U. (2007). Nutritional and Anti- nutritional Compositions of Cleome rutidosperma, Lagenariasiceraria and Cucurbita maxima seeds from Nigeria. J. Med Food, 10:735-738.

[05]    

Fagbemi, T. N. & Oshodi, A. A. (1991). Chemical Composition and Functional Properties of Full Fat Fluted Pumpkin Seed Flour. Nig. Food J., 9:26-32.

[06]    

Asibuao, J. Y., Akromah, R., Safo-kantanka, O., Sei, O., Adu-dapaah, O. S. Hanskof, A. & Agyeman, A. (2008). Chemical compositin of Groundnut (Arachis hypogaea) landracas. African Journal of Biotechnology, 7 (13):2203-2208.

[07]    

Mariod, A. A., Ahmed, Y. M., Matthäus, B., Khaleel, G., Siddig, A., Gabra, A. M. & Abdelwahab, S. I. (2009). A comparative study of the properties of six Sudanese cucurbit seeds and seed oils. J. Am. Oil Chem. Soc., 86(12):1181-1188.

[08]    

Omoti, U. & Okiy, D. A. (1987). Characteristics and composition of the pulp oil and cake of the African pear, Dacryodesedulis. H. J. J. Sci. Food Agric. 38 (1):67-72.





 
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