World Journal of Biochemistry and Molecular Biology  
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Iron and Iron Binding Proteins: Clinical Utilities and Diagnostic Approaches
World Journal of Biochemistry and Molecular Biology
Vol.2 , No. 1, Publication Date: Jun. 7, 2017, Page: 1-7
1024 Views Since June 7, 2017, 451 Downloads Since Jun. 7, 2017
 
 
Authors
 
[1]    

Yoseph Cherinet Megerssa, Department of Biomedical Science, College of Veterinary Medicine and Agriculture, Addis Ababa University, Bishoftu, Ethiopia.

[2]    

Demo Yemane Tesfaye, Department of Medical Laboratory Sciences, College of Health Sciences, Hawassa University, Hawassa Ethiopia.

 
Abstract
 

Iron is important for the function of oxygen-binding molecules and for the electron transport and energy metabolism capacity of cells; deficiency of it causes Iron Deficiency Anemia (IDA). However, the redox activity of iron can also cause damage, primarily by the production of reactive oxygen radicals. Hence, iron concentration is tightly regulated at both cellular and systemic level. Estimation of iron status, thus, is very crucial, especially in woman of child bearing age in developing countries, where iron deficiency is highly prevalent. Different approaches and laboratory techniques have been geared to asses iron status from different angle.


Keywords
 

Iron, Iron Binding Proteins, Iron Deficiency


Reference
 
[01]    

Trefor Higgins, Ernest Beutler, Basil T. Doumas. Hemoglobin, iron and bilirubin. In Carl A. Burtis, Edward R. Ashwood. TEITZ Fundamental of Clinical Chemistry. Saunders, an imprint of Elsevier Inc. 2008; 509-520.

[02]    

Joyce J. C. Kroot, Harold Tjalsma, Robert E. Fleming, Dorine W. Swinkels. Hepcidin in Human Iron Disorders: Diagnostic Implications. Clin Chem 2011; 57(12): 1650-1669.

[03]    

Christian Datz, Thomas Haas, Heinrich Rinner, Friedrich Sandhofer, Wolfgang Patschet al. Heterozygosity for the C282Y mutation in the hemochromatosis gene is associated with increased serum iron, transferrin saturation, and hemoglobin in young women: a protective role against iron deficiency?Clin Chem 1998; 44(12): 2429-24322.

[04]    

James D. Cook, Sandradassenko, Barrys. Skikn. Serum transferrin receptor as an index of iron absorption. Br J Haematol 1990; 75: 603-609.

[05]    

Anthony A. Killeen, John W. Breneman III, Arlene R. Carillo, Jason Liu, Craig S. Hixson. Linked Linear Amplification for Simultaneous Analysis of the Two Most Common Hemochromatosis Mutations. Clin Chem 2003: 49(7): 1050-1057.

[06]    

Dorine W. Swinkels, Mirian C. H. Janssen, Jurgen Bergmans, Joannes J. M. Marx. Hereditary Hemochromatosis: Genetic Complexity and New Diagnostic Approaches. Clin Chem 2006; 52(6): 950-968.

[07]    

Kari Punnonen, Kerttu Irjala, Allan Rajamäki. Serum Transferrin Receptor and Its Ratio to Serum Ferritin in the Diagnosis of Iron Deficiency. Blood 1997; 89: 1052-1057.

[08]    

Hachiro Yamanishi, Shigeki Kimura, Shigeru Iyama, Yoshihisa Yamaguchi, Takehiko Yanagihara. Fully automated measurement of total iron-binding capacity in serum. Clin Chem 1997; 43(12): 2413-2417.

[09]    

Christian Thomas, Lothar Thomas. Biochemical Markers and Hematologic Indices in the Diagnosis of Functional Iron Deficiency. Clin Chem 2002; 48(7): 1066-1076.

[10]    

Ishmael Kasvosve, Joris R. Delanghe, Zvenyika A. R. Gomo, Innocent T. Gangaidzo, Hlosukwazi Khumalo et al. Transferrin Polymorphism Influences Iron Status in Blacks. Clin Chem 2000; 46(10): 1535-1539.

[11]    

Carol H. Flowers, James D. Cook. Dried Plasma Spot Measurements of Ferritin and Transferrin Receptor for Assessing Iron Status. Clin Chem 1999; 45(10): 1826-1832.

[12]    

Babatyi I. Malope, A. Patrick MacPhail, Marianne Alberts, Donavon C. Hiss. The ratio of serum transferrin receptor and serum ferritin in the diagnosis of iron status. Br J Haematol 2001; 115: 84-89.

[13]    

Pauli Suominen, Kari Punnonen, Allan Rajamäki, Kerttu Irjala. Serum Transferrin Receptor and Transferrin Receptor-Ferritin Index Identify Healthy Subjects With Subclinical Iron Deficits. Blood 1998; 92: 2934-2939.

[14]    

Leif Hallberg, Calle Bengtsson, Leif Lapidus, Horan Lindstedt, Per-Arne Lundbers, et al. Screening for iron deficiency: an analysis based on bone-marrow examinations and serum ferritin determinations in a population sample of women. Br J Heamatol 1993; 85: 787-798.

[15]    

Alan E. Mast, Morey A. Blinder, Ann M. Gronowski, Cara Chumley, Mitchell G. Scott. Clinical utility of the soluble transferrin receptor and comparison with serum ferritin in several populations. Clin Chem 1998; 44(1): 45-51.

[16]    

Jean Allen, Kristin R. Backstrom, Jeffrey A. Cooper, MaryAnne C. Cooper, Thomas C. Detwiler, et al. Measurement of soluble transferrin receptor in serum of healthy adults. Clin Chem 1998; 44 (1) 35-39.

[17]    

F. J. Baillie, A. E. Morrison, I. Fergus. Soluble transferrin receptor: a discriminating assay for iron deficiency. Clin lab Haematol 2003; 25: 353-357.

[18]    

Hlosukwazi Khumalo, Zvenyika A. R. Gomo, Victor M. Moyo, Victor R. Gordeuk, Thokozile Saungweme, et al. Serum transferrin receptors are decreased in the presence of iron overload. Clin Chem 1998; 44(1): 40-44.

[19]    

Alan E. Mast, Morey A. Blinder, Qing Lu, Sherri Flax, Dennis J. Dietzen. Clinical utility of the reticulocyte hemoglobin content in the diagnosis of iron deficiency. Blood 2002; 99: 1489-1491.

[20]    

Christian Thomas, Uwe Kobold, Lothar Thomas. Serum hepcidin-25 in comparison to biochemical markers and hematological indices for the differentiation of iron-restricted erythropoiesis. Clin Chem Lab Med 2011; 49(2): 207-213.

[21]    

Elisa Piva, Carlo Brugnara, Lino Chiandetti, Mario Plebani. Automated reticulocyte counting: state of the art and clinical applications in the evaluation of erythropoiesis. Clin Chem Lab Med 2010;48(10):1369–1380.

[22]    

Mauro Buttanrello, pietro Bullan, Marina De Pra, Pietro Barbera, Paolo Rizzotti. Reticulocyte Quantification by Coulter MAXM VCS (Volume, conductivity, light scatter) technology. Clin Chem 1996; 42(12): 1930-1937.

[23]    

Jose ́ F. A. Noronha, Helena Z. W. Grotto. Measurement of reticulocyte and red blood cell indices in patients with iron deficiency anemia and b-thalassemia minor. Clin Chem Lab Med 2005;43(2):195–197.

[24]    

Joyce J. C. Kroot, Coby M. M. Laarakkers, Anneke J. Geurts-Moespot, Nicolaı Grebenchtchikov, Peter Pickkers, et al. Immunochemical and Mass-Spectrometry–Based Serum Hepcidin Assays for Iron Metabolism Disorders. Clin Chem 2010; 56(10): 1570-1579.

[25]    

Naohisa Tomosugi, Hiroshi Kawabata, Rumi Wakatabe, Masato Higuchi, Hideki Yamaya, Hisanori Umehara, et al. Detection of serum hepcidin in renal failure and inflammation by using ProteinChip System®. Blood 2005; 10: 4043.

[26]    

Erwin Kemna, Harold Tjalsma, Coby Laarakkers, Elizabeta Nemeth, Hans Willems et al. Novel urine hepcidin assay by mass spectrometry. Blood 2005; 05: 1873.

[27]    

Ivana De Domenico, Elizabeta Nemeth, Jenifer M. Nelson, John D. Phillips, Richard S. Ajioka, et al. The Hepcidin-Binding Site on Ferroportin Is Evolutionarily Conserved. Cell Metab 2008; 8: 146-156.

[28]    

Anthony M. Butterfield, Peng Luan, Derrick R. Witcher, Joseph Manetta, Anthony T. Murphy, et al. A Dual-Monoclonal Sandwich ELISA Specific for Hepcidin-25. Clin Chem 2010; 56(11): 1725-1732.





 
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