World Journal of Biochemistry and Molecular Biology  
Manuscript Information
 
 
Silibinin Is an Inhibitor of miR-24-3p Gene Expression in T47D Breast Cancer Cell Line
World Journal of Biochemistry and Molecular Biology
Vol.1 , No. 2, Publication Date: Sep. 24, 2016, Page: 6-10
2260 Views Since September 24, 2016, 510 Downloads Since Sep. 24, 2016
 
 
Authors
 
[1]    

Fatemeh Khaloozade, Kish International Campus, University of Tehran, Tehran, Iran.

[2]    

Nasrin Motamed, School of Biology, College of Science, University of Tehran, Tehran, Iran.

[3]    

Elnaz Birjandian, School of Biology, College of Science, University of Tehran, Tehran, Iran.

 
Abstract
 

MiR-24-3p has been considered as an attractive molecular target for breast cancer therapy. The main objective of the present study was to assess the inhibitory effects of silibinin, a herbal component, on the proliferation, apoptotic induction and the expression of miR-24-3p gene in T47D human breast cancer cell line. Human breast cancer cell line of T47D was treated with various concentrations of silibinin. Cellular viability and the apoptotic rate were assessed by MTT assay flow cytometric analysis, respectively. Expression of human miR-24-3p gene was measured with real-time PCR. The present study has demonstrated that the silibinin inhibited the cellular growth in a dose- and time-dependent manner, and effectively induced a considerable amount of apoptosis in of T47D cells. In addition, silibinin caused a decrease in miR-24-3p expression. The present findings suggest that silibinin could successfully reduce the cell viability and miR-24-3p gene-expression in breast cancer cells. These antineoplastic effects of silibinin were strengthened by its inhibiting effects on the cell proliferation, and apoptosis-induced down-regulation of miR-24-3p expression in T47D cells.


Keywords
 

Silibinin, Breast Cancer, T47D Cell Line, miR-24-3p


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