Indexing
All Issues
Submission
Author Guidelines
Reviewers
Editorial Members
Publication Fee
Vol.4 , No. 2, Publication Date: May 30, 2018, Page: 9-16
 <i>Monech</i>) Genotypes&description=Protein changes induced by water stress during growth and development of sorghum (<i>Sorghum bicolor</i> (L.) <i>Monech</i>) were studied in afield experiment conducted for two seasons (2014-15 and 2015-16) at the demonstration farm, Faculty of Agriculture, University of Khartoum. The design was split plot with three replication. The main plots were assigned to water treatments and the subplots to the cultivars. Water stress was induced by irrigation at intervals of 7 (W1), 14 (W2) and 21 (W3) days. The following local sorghum cultivars: Wad Ahmed, Arfa Gadamak, Tabbat, Abu Sabien and White Mugud were used. Leaves were collected at random to study changes in protein expression during flowering and physiological maturity stages. As a result of water stress, different types of proteins, mainly with low molecular weight (ranging from 14-117KDa), were induced in all studied cultivars. The degradation of proteins (ranging from 44-77KDa) was also found in some cultivars in response to water stress. Genotypic variations were observed for a large number of proteins induced at flowering in Wad Ahmed and at physiological maturity in White Mugud and Tabbat.&picture=http://www.aascit.org/aascit/decorators/img/journal/971.jpg)
| [1] | Huda Abdu Hassan Osman, Department of Botany and Agric. Biotechnology, Faculty of Agriculture, University of Khartoum, Shambat, Sudan. |
| [2] | Eltayeb Hag Ali Ahmed, Department of Botany and Agric. Biotechnology, Faculty of Agriculture, University of Khartoum, Shambat, Sudan. |
| [3] | Elhadi Ali Ibrahim Elkhalil, Department of Botany and Agric. Biotechnology, Faculty of Agriculture, University of Khartoum, Shambat, Sudan. |
Protein changes induced by water stress during growth and development of sorghum (Sorghum bicolor (L.) Monech) were studied in afield experiment conducted for two seasons (2014-15 and 2015-16) at the demonstration farm, Faculty of Agriculture, University of Khartoum. The design was split plot with three replication. The main plots were assigned to water treatments and the subplots to the cultivars. Water stress was induced by irrigation at intervals of 7 (W1), 14 (W2) and 21 (W3) days. The following local sorghum cultivars: Wad Ahmed, Arfa Gadamak, Tabbat, Abu Sabien and White Mugud were used. Leaves were collected at random to study changes in protein expression during flowering and physiological maturity stages. As a result of water stress, different types of proteins, mainly with low molecular weight (ranging from 14-117KDa), were induced in all studied cultivars. The degradation of proteins (ranging from 44-77KDa) was also found in some cultivars in response to water stress. Genotypic variations were observed for a large number of proteins induced at flowering in Wad Ahmed and at physiological maturity in White Mugud and Tabbat.
Keywords
Protein, Water Stress, Sorghum
Reference
| [01] | Azevedo, R. A., Damerval, C, Landry, J., Lea, P. J., Bellato, C. M., Meinhardt, L. W., Le Guilloux, M., Delhaye, S., Toro, A. A., Gaziola, S. A. and Berdejo, B. D. A. (2003). Regulation of maize lysine metabolism and endosperm protein synthesis by opaque and floury mutations. Eur. J. Biochem. 270: 4898-4908. |
| [02] | Azevedo, R. A., Damerval, C., Lea, P. J., Landry, J., Bellato, C. M., Meinhardt, L. W., Le Guilloux, M., Delhaye, S., Toro, A. A., Gaziola, S. A., Varisi, V. A. and Grat, P. L. (2004). Genetic control of lysine metabolism in maize endosperm mutants. Funct. Plant Biol. 31: 339-348 |
| [03] | Chang, S., Puryear, J. D., Madl, D., Funkhouser, E. A., Newton, R. J. andCairney, J. (1995). Gene expression under water deficit in loblolly pine (Pinus taeda L.): isolation and characterization of Cdna clones. Physiol Plant 95: 1–10. |
| [04] | Riccardi, F., Gazeau, P. and Vienne, D. (1998). Protein changes in response to progressive water deficit in maize, quantitative variation and polypeptide identification. Plant Physiol 117: 1253-1263. |
| [05] | Laemmli, U. K. (1970). Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Nature (London), 277: 680-685. |
| [06] | Kiegle, E., Moore, C. A., Haseloff, J., Tester, M. A. and Knight, M. R. (2000). Cell-type specific calcium responses to drought, salt and cold in the Arabidopsis root. Plant Journal, 23: 267-278. |
| [07] | Biba, N., Hameed, A., Ali, H., Iqbal, N., Haq, M. A., Atta, B. M., Shah, T. M. and Alam, S. S. (2009). Water stress induced variations in protein profiles of germinating cotyledons from seedlings of chickpea genotypes. Pak. J. Bot., 41 (2): 731-736. |
| [08] | Yamaguchi-Shinozaki, K. and Shinozaki, K. (1993). Arabidopsis DNA Encoding Two Desiccation-Responsive rd 29 Genes. Plant Physiol., 101: 1119-1120. |
| [09] | Fray, R. G., Wallace, A., Grierson, D. and Lycett, G. W. (1994). Nucleotide sequence and expression of a ripening and water stress-related cDNA from tomato with homology to the MIP class of membrane channel proteins. Plant Mol Biol 24: 539–543. |
| [10] | Abu-Romman, S. (2016). Genotypic response to heat stress in durum wheat and the expression of small HSP genes. Rendiconti Lincei, 27 (2): 261-271. |
| [11] | Nover, L., Neumann, D. and Scharf, K. D. (1989). Heat shock and other stress response systems of plants. Results and Problems in Cell Differentiation, Vol. 16. Springer – Verlag, Berlin. |
| [12] | Wahid, A. and Close, T. J. (2007). Expression of dehydrins under heat stress and their relationship with water relations of sugar cane leaves. Biol. Plant, 51: 104-109. |
| [13] | Schilirò, E. (2000). Stress proteins and identification of interacting partners in the resurrection plant Craterostigma plantagineum. Ph.D. Thesis, Mathemathisch-Naturwissenschaftlichen Fakultät der Universität zu Köln, Germany. |
| [14] | Song, Y.; Chen, Q.; Ci, D.; Shao, X. and Zhang, D. (2014). Effects of high temperature on photosynthesis and related gene expression in poplar. BMC Plant Biology, 14: 111-131. |
| [15] | Bukau, B., Weissman, J. and Horwich, A. (2006). Molecular chaperones and protein quality control. Cell 125: 443-451. |
| [16] | Wood, A. J. and Goldsbrough, P. B. (2006). Characterization and expression of dehydrins in water stressed Sorghum bicolor. Physiology Planturm., 99: 144-152. |
| [17] | Sharma, A. D., Wajapeyee, N., Yadav, V. and Singh, P. (2004). Stress-Induced Changes in Peptidyl-Prolyl cis-trans Isomerase Activity of Sorghum bicolor Seedlings. Biologia Plantarum, 47 (3): 367-371. |
| [18] | Muhammadkhan, N. and Heidari, R.(2008). Effect of drought stress on soluble proteins in two maize varieties. Turk J Biol 32: 23-30. |
| [19] | Bibi, A., Sadaqat, H. A., Akram, H. M. and Mohammed, M. I. (2010). Physiological Markers for Screening Sorghum (Sorghum bicolor) Germplasm under Water Stress Condition. Inter. J. of Agric. And Biol., 12 (3): 451-455. |
| [20] | Abid, G; Muhovski, Y; Mingeot, D.; Watillon, B.; ´Toussaint, A.; Mergeai, G.; M’hamdi, M.; Sassi, K. and Jebara, M. (2015). Identification and characterization of drought stress responsive genes in faba bean (Vicia faba L.) by suppression subtractive hybridization. Plant Cell Tissue and Organ Culture, 121 (2): 367-379. |
