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Vol.3 , No. 3, Publication Date: Aug. 30, 2017, Page: 16-23
 were produced by <i>Lactococcus lactis</i> RCM21 using a modified de Man, Rogosa and Sharpe (MRS) media. Pyocyanin (70µg/ml) was synthesised by <i>Pseudomonas aeruginosa</i> OB11 in culture extracts using glycerol-supplemented cetrimide media, extracted and confirmed by FT-IR spectroscopy. Agar diffusion method was adopted and pyocyanin/bacteriocin time-based single and combined applications had varying antimicrobial effects against <i>Staphylococcus aureus</i> ATCC 29213, <i>Escherichia coli</i> ATCC 25922 and <i>Candida albicans</i> ATCC 10261. Zone of clearing measurements (mm) on agar plates showed that combined use led to the highest inhibition (28.3±0.38 mm) against <i>S. aureus</i> when pyocyanin/bacteriocin combination of 50µl/50µl at 72h contact time, while the lowest inhibition (13.8±0.33 mm) was obtained against <i>E. coli</i> when a combination of 30µl/70µl at 24h contact time was used. In single use, no inhibition was observed when 100µl of bacteriocin fluid was applied against <i>C. albicans</i>. However, values ranging from between 2.5±0.61mm and 14.5±0.81mm were observed when pyocyanin and bacteriocin were used singly on the different test organisms at varying contact times, thus showing a less potent activity of the single when compared with the combined. Antimicrobial values for the singly applied bacteriocin or pyocyanin were lower than values obtained for the combinations used in all experiments, implying synergism. Physiologically, flame photometry also showed that the co-application of the two antimicrobials led to a higher effect of sodium and potassium leakage from cells of the test organisms than single application. Pyocyanin and bacteriocins possess good potentials for further efficacious development especially as combined therapy.&picture=http://www.aascit.org/aascit/decorators/img/journal/971.jpg)
| [1] | Ajunwa Obinna Markraphael, Department of Microbiology, Modibbo Adama University of Technology, Yola, Nigeria. |
| [2] | Ewansiha Joel Uyi, Department of Microbiology, Modibbo Adama University of Technology, Yola, Nigeria. |
| [3] | Audu Jemilatu Omuwa, Department of Laboratory Technology, Modibbo Adama University of Technology, Yola, Nigeria. |
| [4] | Pukuma Musa Sale, Department of Microbiology, Modibbo Adama University of Technology, Yola, Nigeria. |
| [5] | Ja’afaru Mohammed Inuwa, Department of Microbiology, Modibbo Adama University of Technology, Yola, Nigeria. |
Bacteriocin fluids (4274 AU/ml) were produced by Lactococcus lactis RCM21 using a modified de Man, Rogosa and Sharpe (MRS) media. Pyocyanin (70µg/ml) was synthesised by Pseudomonas aeruginosa OB11 in culture extracts using glycerol-supplemented cetrimide media, extracted and confirmed by FT-IR spectroscopy. Agar diffusion method was adopted and pyocyanin/bacteriocin time-based single and combined applications had varying antimicrobial effects against Staphylococcus aureus ATCC 29213, Escherichia coli ATCC 25922 and Candida albicans ATCC 10261. Zone of clearing measurements (mm) on agar plates showed that combined use led to the highest inhibition (28.3±0.38 mm) against S. aureus when pyocyanin/bacteriocin combination of 50µl/50µl at 72h contact time, while the lowest inhibition (13.8±0.33 mm) was obtained against E. coli when a combination of 30µl/70µl at 24h contact time was used. In single use, no inhibition was observed when 100µl of bacteriocin fluid was applied against C. albicans. However, values ranging from between 2.5±0.61mm and 14.5±0.81mm were observed when pyocyanin and bacteriocin were used singly on the different test organisms at varying contact times, thus showing a less potent activity of the single when compared with the combined. Antimicrobial values for the singly applied bacteriocin or pyocyanin were lower than values obtained for the combinations used in all experiments, implying synergism. Physiologically, flame photometry also showed that the co-application of the two antimicrobials led to a higher effect of sodium and potassium leakage from cells of the test organisms than single application. Pyocyanin and bacteriocins possess good potentials for further efficacious development especially as combined therapy.
Keywords
Bacteriocin, Pyocyanin, Lactococcus lactis, Pseudomonas aeruginosa
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