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AASCIT Journal of Bioscience

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Gene Expression Microarray Data from Late Endothelial Progenitor Cells

AASCIT Journal of Bioscience
Vol.2 , No. 4, Publication Date: Aug. 12, 2016, Page: 34-41

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Authors

[1]	Dan Zhang, Department of Biotechnology, Jinan University, Guangzhou, China.
[2]	Longqiu Cheng, Department of Biotechnology, Jinan University, Guangzhou, China.

Abstract

Numerous in vitro and in vivo studies imply that the late EPCs released from bone marrow can participate in endothelial repair and angiogenesis. However, the molecular basis or signals causing their migration and homing to sites of mature vascular endothelium or extravascular tissue are largely unclear at present. To investigate the molecular basis, we studied the gene expression profile in late EPCs. We used Transcriptomic microarray analysis to detect the expression of genes in three EPCs and three HUVEC samples, and differentially expressed genes were identified through Volcano Plot filtering. The function of differentially expressed genes was determined by pathway and Gene ontology (GO) analyses. A total of 2941 genes were found to be differentially expressed between late EPCs and HUVEC groups. HUVEC as control, the late EPCs exhibited different gene expression profile, GO and pathway analyses showed that the functions of differentially expressed genes were closely related with many processes which involved in the development of angiogenesis.

Keywords

Late Endothelial Progenitor Cells (Late EPCs), Human Transcriptome Array 2.0 (HTA 2.0), Differentially Expressed Genes, Up-Regulated Genes, Down-Regulated Genes

Reference

[01]	Carmen Urbich, Stefanie Dimmeler. Endothelial Progenitor Cells: Characterization and Role in Vascular Biology [J]. Circulation Research. 2004, 95(4):343-353.
[02]	Mihail Hristov, Wolfgang Erl, Peter C. Weber. Endothelial progenitor cells: mobilization, differentiation, and homing [J]. Arterioscler Thromb Vasc Biol. 2003, 23(7):1185-9.
[03]	Canan Alkim, Huseyin Alkim, Ali Riza Koksal, et al. Angiogenesis in Inflammatory Bowel Disease [J]. International journal of inflammation. 2015, 1155: 1-10.
[04]	I. D. Pousa, J. Mat e, and J. P. Gisbert. Angiogenesis inflammatory bowel disease [J]. European Journal of Clinical Investigation. 2008, 38:73-81.
[05]	ANN HOEBEN, BART LANDUYT, MARTIN S, et al. Vascular Endothelial Growth Factor and Angiogenesis [J]. Pharmacological reviews. 2004, 56(4):549-80.
[06]	Assmus B. Transplantation of Progenitor Cells and Regeneration Enhancement in Acute Myocardial Infarction (TOPCARE-AMI) [J]. Circulation. 2002, 106(24):3009-17.
[07]	Jianwen Zhu, Xiangwei Cheng, Qianhua Wang, et al. Transplantation of endothelial progenitor cells for improving placental perfusion in preeclamptic rats [J]. Archives of gynecology and obstetrics. 2015, 291(5):1113-9.
[08]	Murohara T, Ikeda H, Duan J, et al. Transplanted cord blood-derived endothelial precursor cells augment postnatal neovascularization [J]. The Journal of clinical investigation. 2000, 105(11):1527-36.
[09]	Jennifer M, Hughes-Large, Nica M. Borradaile. Gene expression microarray data from human microvascular endothelial cells supplemented with a low concentration of niacin. [J]. Elsevier. 2016, 6:899-902.
[10]	Asahara T, Murohara T, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis [J]. Science. 1997, 275(5302):964-7.
[11]	Hur J, Yoon CH, Kim HS, et al. Characterization of Two Types of Endothelial Progenitor Cells and Their Different Contributions to Neovasculogenesis [J]. Arteriosclerosis, thrombosis, and vascular biology. 2003, 24(2):288-93.
[12]	Hristov M, Erl W, Weber PC. Endothelial progenitor cells: isolation and characterization. [J]. Trends Cardiovasc Med. 2003, 13(5):201-6.
[13]	U. Fiedler, Y. Reiss, M. Scharpfenecker, et al. Angiopoietin-2 sensitizes endothelial cells to TNF-alpha and has a crucial role in the induction of inflammation [J]. Nature medicine. 2006, 12:235-9.
[14]	N. Ferrara, H. P. Gerber, and J. Le Couter. The biology of VEGF and its receptors [J]. Nature medicine. 2003, 9:669-76.
[15]	E. Shimshoni, D. Yablecovitch, L. Baram, et al. ECM remodeling in IBD: innocent bystander or partner in crime? The emerging role of extracellular molecular events in sustaining intestinal inflammation [J]. Gut. 2015, 64:367-72.