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AASCIT Journal of Bioscience

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Captured Proteins by Immobilized Ricinus Communis Agglutinin in Rabbit Plasma

AASCIT Journal of Bioscience
Vol.2 , No. 3, Publication Date: Jun. 6, 2016, Page: 30-33

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Authors

[1]	Manhua Liu, Department of Biotechnology, Jinan University, Guangzhou, China.
[2]	Xueke Zhou, Department of Biotechnology, Jinan University, Guangzhou, China.
[3]	Tingting Wang, Department of Biotechnology, Jinan University, Guangzhou, China.
[4]	Renqiang Li, Department of Biotechnology, Jinan University, Guangzhou, China.

Abstract

The Sepharose CL-6B column was prepared to separate ricinus communis agglutinin (RCA) from the crude extract of castor beans due to the gel carrier contains a large amount of β-galactoside bond. RCA were immobilized on activated Sepharose 4B to make an immobilized RCA column, which was used to capture the proteins in rabbit plasma by affinity chromatography. Results showed that two polypeptides with molecular mass about 120 and 97 KDa were captured by RCA in rabbit plasma. The captured polypeptides were demonstrated to contain the carbohydrate. The method performed in this study is much useful in the separation of sugary proteins.

Keywords

Sepharose CL-6B, Immobilized RCA, Captured Proteins by RCA, Affinity Chromatography, Plasma

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