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Vol.4 , No. 6, Publication Date: Nov. 14, 2017, Page: 50-58
. Initial studies of the UV-spectra of the individual compounds showed that satisfactory linear regression calibration models could be constructed in the concentration range of 5-50µg/ml and 2-20µg/ml for candesartan and hydrochlorothiazide respectively. Resolution of binary mixtures of the drugs has been accomplished by using <sup>1</sup>D (first derivative), <sup>1</sup>D ratio (first derivative ratio). The multivariate methods, classical least squares (CLS) and principal components regression (PCR) has also been used for determination the studied drugs. Thus, a simple, rapid and inexpensive method for the simultaneous determination of these drugs (either in their pure powder forms, laboratory prepared samples or in their pharmaceutical formulation) was researched and developed with the aid of chemometrics assisted spectrophotometric methods.&picture=http://www.aascit.org/aascit/decorators/img/journal/922.jpg)
| [1] | Yitayal Admassu Workie, Pharmaceutical Chemistry Stream, Department of Industrial Chemistry, College of Applied Science, Addis Ababa Science and Technology University, Addis Ababa, Ethiopia. |
| [2] | Abd El-Maaboud Ismael Mohamed, Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Assiut University, Assiut, Egypt. |
| [3] | Adnan Ahmed Bekhit, Pharmaceutical Chemistry Department, Faculty of Pharmacy, Alexandria University, Alexandria, Egypt. |
| [4] | Ariaya Hymete, Pharmaceutical Chemistry Department, School of Pharmacy, Addis Ababa University, Addis Ababa, Ethiopia. |
The simultaneous determination of candesartan cilexetil and hydrochlorothiazide mixtures by using spectrophotometric method is a difficult task in analytical chemistry, due to spectral interferences. Ultra violet-spectra of the studied drugs were collected from 200 nm to 350 nm in ethanol solutions. In this range the studied drugs showed a considerable degree of spectral overlapping (91.82%). Initial studies of the UV-spectra of the individual compounds showed that satisfactory linear regression calibration models could be constructed in the concentration range of 5-50µg/ml and 2-20µg/ml for candesartan and hydrochlorothiazide respectively. Resolution of binary mixtures of the drugs has been accomplished by using 1D (first derivative), 1D ratio (first derivative ratio). The multivariate methods, classical least squares (CLS) and principal components regression (PCR) has also been used for determination the studied drugs. Thus, a simple, rapid and inexpensive method for the simultaneous determination of these drugs (either in their pure powder forms, laboratory prepared samples or in their pharmaceutical formulation) was researched and developed with the aid of chemometrics assisted spectrophotometric methods.
Keywords
Chemometrics, Candesartan Cilexetil, Hydrochlorothiazide, First Derivative, First Derivative Ratio, Classical Least Square, Principal Component Regression
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