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American Journal of Pharmacy and Pharmacology

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In Vivo Activity of Fractions of Combretum Molle R. and Haematological Profile of Trypanosoma Brucei Brucei Infected Mice

American Journal of Pharmacy and Pharmacology
Vol.4 , No. 4, Publication Date: Jul. 5, 2017, Page: 15-20

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Authors

[1]	Ubandoma Andefiki, Department of Microbiology, Federal University Wukari, Wukari, Nigeria.
[2]	Inabo Helen Ileigo, Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria.
[3]	Abdullahi Isah, Department of Microbiology, Ahmadu Bello University, Zaria, Nigeria.

Abstract

The in vivo antitrypanosomal potentials of Combretum molle were investigated. The in-vivo activity of the fractions at (15mg/kg and 10mg/kg) were compared with standard diminazene aceturate (Diminal) at (3.5mg/Kg), both methanolic and aqueous fractions containing saponins and flavonoids respectively were also fractionated and used for intraperitoneal treatment of mice infected with Trypanosoma brucei brucei federe strain. The Lethal Dose (LD₅₀) were (28.98mg/kg) and (14.14mg/kg) respectively for both methanolic and aqueous crude extracts. The haematological profile showed a significant difference (t-test =9.116; p-value =0.001) between the pre-infection and post infection and treatment values of packed cell volume (PCV) of the infected mice. However, there was no significant difference in the values of neutrophils, lymphocytes as well as the total white blood cell (WBC) counts. The results showed that Combretum molle had mild in vivo activity, hence can serve as a promising candidate for drug development against trypanosomosis.

Keywords

Mice, Trypanosoma, Intraperitoneal, Haematological Profile, Combretum molle, Antitrypanosomal Property, Nagana

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