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American Journal of Pharmacy and Pharmacology

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Functional Assessments and Histopathology of Hepatorenal Tissues of Wistar Rats Infused with Carbon Tetrachloride and Treated with Raw and Hydrothermal Processed Herbs

American Journal of Pharmacy and Pharmacology
Vol.4 , No. 5, Publication Date: Aug. 8, 2017, Page: 21-34

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Authors

[1]	Okey Alphonsus Ojiako, Department of Biochemistry, Federal University of Technology, Owerri, Nigeria.
[2]	Paul Chidoka Chikezie, Department of Biochemistry, Imo State University, Owerri, Nigeria.
[3]	Doris Ifeyinwa Ukairo, Department of Biochemistry, Federal University of Technology, Owerri, Nigeria.
[4]	Chiedozie Onyejiaka Ibegbulem, Department of Biochemistry, Federal University of Technology, Owerri, Nigeria.
[5]	Reginald Nwazue Nwaoguikpe, Department of Biochemistry, Federal University of Technology, Owerri, Nigeria.

Abstract

The present study ascertained the functional integrity of hepatic and renal tissues, concurrently with blood lipid patterns, of Wistar rats infused with CCl₄ and treated with raw and hydrothermal processed herbs, namely, Monodora myristica, Chromolaena odorata, Buccholzia coriacea and Sphenostylis stenocarpa. Measurement of phytochemical contents of the herbs was according to standard methods. The rats were randomly designated on the bases of diets and treatments received for 28 consecutive days. Fibrosis was induced in the Wistar rats by single dose intra-peritoneal injection of CCl₄ for 2 consecutive days. Liver and kidney function tests and serum lipid profile were measured using spectrophotometric methods. Renal and hepatic tissues were subjected to histopathological examinations. The concentrations of alkaloids in the four herbal extracts were within the range of 4.83 ± 0.03 – 31.33 ± 0.29 mg/100 g sample, whereas the concentrations of saponins varied within a relatively narrow range: 0.33 ± 0.09 – 4.33 ± 0.02 mg/100 g dry sample; p > 0.05. The activity ratios of AST to ALT of the rat groups were generally less than 1.0 unit. Atherogenic indices of fibrotic rats were within the following ranges: TAG/HDL-C ratio (3.59 ± 0.03 – 6.76 ± 0.06), TC/HDL-C ratio (3.72 ± 0.02 – 6.94 ± 0.05) and LDL-C/HDL-C ratio (2.00 ± 0.01 – 4.59 ± 0.02). Losses in phytochemical contents following hydrothermal processing of the herbs did not substantially affect their overall therapeutic scores against morphological and functional impairments of hepatic and renal tissues following CCl₄ intoxication of the rats.

Keywords

Carbon Tetrachloride, Histopathology, Hydrothermal, Kidney, Liver

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