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Vol.2 , No. 5, Publication Date: Feb. 3, 2016, Page: 61-71
 Induced Acute Liver Injury in Wistar Rats&description=The study was designed to investigate the effect of aqueous whole plant extract of <i>selaginellamyosurus</i> on liver and haematological parameters of carbon tetrachloride (CCl<sub>4</sub>) induced hepatotoxicity in wistar rats. Fifty (52) wistarrats of both sexes weighing 150-175g were divided into thirteen groups (±4g/group). Group 1 received distil water only, 2 and 3 received CCl<sub>4</sub>only, 4-7 received varying doses of 400 and 800mg/kgbw of extract, 8-11 received varying doses of 400 and 800mg/kgbw of extract after CCl<sub>4</sub>treatment and 12 and 13 received 100mg/kgbw of vitamin C after treating with CCl<sub>4</sub>all for 7 and 14 days. Rats were sacrificed and blood samples were collected for some biochemical and haematological analyses, liver was collected for histological investigation while phytochemical screening was conducted on the plant. Result showed significant elevation (p<0.05) of ALT, AST and ALP activities and TP level and non significant differences (p>0.05) in albumin and bilirubin levels when groups 2 and 3 were compared to group 1. Treatment with different doses of extract alone in all the groups did not show any significant change in all the parameters when compared to group 1. Groups 4, 5, 6, and 7 revealed non significant reduction (p>0.05) in the activities of ALT, AST and ALP and TP level when compared to groups 2 and 3. Vit C treatment for 14 days returned values of all the parameters to normal values. Groups 2-9 demonstrated non significant differences in Hb level, PCV, and RBC count when compared to control group. WBC, platelet and leukocyte counts significantly reduced (p<0.05) when some of the groups were compared to control group. Quantitative phytochemical screening of plant revealed the presence of flavonoids, triterpenoids, saponins, tannin, steroid, cardiac glycoside, phenol and absence of alkaloids, anthraquinone and cyanogenic glycosides. Histology result revealed regeneration of CCl<sub>4</sub>induced hepatocyte damage in some of the extract treated group. Therefore aqueous whole plant extract of <i>selagenellamyosurus</i> is safe at these doses and has ameliorating effect on CCl<sub>4</sub>induced hepatotoxicity.&picture=http://www.aascit.org/aascit/decorators/img/journal/922.jpg)
| [1] | Peters D. E., Department of Biochemistry, Faculty of Science, University of Port Harcourt, Rivers State, NigeriaDepartment of Biochemistry, Faculty of Science, University of Port Harcourt, Rivers State, Nigeria. |
| [2] | Omeodu S. I., Department of Biochemistry, Faculty of Science, University of Port Harcourt, Rivers State, Nigeria. |
| [3] | Egbordi C. E., Department of Biochemistry, Faculty of Science, University of Port Harcourt, Rivers State, Nigeria. |
The study was designed to investigate the effect of aqueous whole plant extract of selaginellamyosurus on liver and haematological parameters of carbon tetrachloride (CCl4) induced hepatotoxicity in wistar rats. Fifty (52) wistarrats of both sexes weighing 150-175g were divided into thirteen groups (±4g/group). Group 1 received distil water only, 2 and 3 received CCl4only, 4-7 received varying doses of 400 and 800mg/kgbw of extract, 8-11 received varying doses of 400 and 800mg/kgbw of extract after CCl4treatment and 12 and 13 received 100mg/kgbw of vitamin C after treating with CCl4all for 7 and 14 days. Rats were sacrificed and blood samples were collected for some biochemical and haematological analyses, liver was collected for histological investigation while phytochemical screening was conducted on the plant. Result showed significant elevation (p<0.05) of ALT, AST and ALP activities and TP level and non significant differences (p>0.05) in albumin and bilirubin levels when groups 2 and 3 were compared to group 1. Treatment with different doses of extract alone in all the groups did not show any significant change in all the parameters when compared to group 1. Groups 4, 5, 6, and 7 revealed non significant reduction (p>0.05) in the activities of ALT, AST and ALP and TP level when compared to groups 2 and 3. Vit C treatment for 14 days returned values of all the parameters to normal values. Groups 2-9 demonstrated non significant differences in Hb level, PCV, and RBC count when compared to control group. WBC, platelet and leukocyte counts significantly reduced (p<0.05) when some of the groups were compared to control group. Quantitative phytochemical screening of plant revealed the presence of flavonoids, triterpenoids, saponins, tannin, steroid, cardiac glycoside, phenol and absence of alkaloids, anthraquinone and cyanogenic glycosides. Histology result revealed regeneration of CCl4induced hepatocyte damage in some of the extract treated group. Therefore aqueous whole plant extract of selagenellamyosurus is safe at these doses and has ameliorating effect on CCl4induced hepatotoxicity.
Keywords
Haematology, Hepatotoxicity, Carbon Tetrachloride, Alanine Amino Transferase (ALT), Alkaline Phosphatase (ALP), Red Blood Cell (RBC), White Blood Cell (WBC), Packed Cell Volume (PCV)
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