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Vol.1 , No. 2, Publication Date: Oct. 21, 2014, Page: 56-63
| [1] | Malik Altaf Hussain, The Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 7647, New Zealand. |
| [2] | Navdeep Kaur Natt, The Department of Wine, Food and Molecular Biosciences, Lincoln University, Lincoln 7647, New Zealand. |
Proteomic analysis was carried out to identify and characterize protein expression in Lactobacillus rhamnosus in response to starvation and low pH stress. The cytosolic proteomes of L. rhamnosus cells grown in modified MRS broth under control, lactose starvation and acid stress conditions were analysed. Gel-free proteomic analysis using iTRAQ-LC-MS/MS revealed 25 and 27 proteins, produced by starved and acid stressed cells respectively, were differentially expressed in comparison to cells grown in control culture condition. Starved cells produced high amounts of enzymes with functionalities in gluconeogenesis, amino acid metabolism, fatty acid bio-synthesis and sugar metabolisms other than glycolysis. Acid stressed cells showed down-regulations of more proteins than starved cells. The data suggest that L. rhamnosus uses two different sets of proteins to cope with each of the stress condition. The results revealed 63% of up-regulated proteins in starved cells were associated with alternative carbon or energy source scavenging activity. Good understanding of the adaptation mechanisms in Lactobacillus species during the starvation and other stress conditions is important for successful use of these microorganisms in health and industry.
Keywords
Gel-Free Proteomics, iTRAQ Analysis, Lactobacillus rhamnosus, Starvation, Acid Stress, Amino Acid Metabolism
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