ISSN: 2375-3005
American Journal of Microbiology and Biotechnology  
Manuscript Information
 
 
Dissimilatory Sulfate Reduction in Bacterium Desulfovibrio Piger Vib-7 under the Effect of Medium with Differential Acidity
American Journal of Microbiology and Biotechnology
Vol.1 , No. 2, Publication Date: Oct. 16, 2014, Page: 49-55
1571 Views Since October 16, 2014, 1091 Downloads Since Apr. 14, 2015
 
 
Authors
 
[1]    

Ivan Kushkevych, Laboratory of Molecular Biology and Clinical Biochemistry, Institute of Animal Biology of NAAS of Ukraine.

 
Abstract
 

In this paper, the effect of cultivation medium with differential acidity on the dissimilatory sulfate reduction process in bacterium Desulfovibrio piger Vib-7 isolated from the human intestine was studied. Microbiological, biochemical, and biophysical methods of the studies, and statistical processing of the results were used; the obtained data were compared with those from literature. The most intensive growth of intestinal bacterium D. piger Vib-7, sulfate and lactate dissimilation, and sulfide and acetate production was determined at the optimum pH 7.0–8.0. A correlation and cross-correlation analysis of these processes under the effect of differential acidity of the culture medium was carried out. The described results of these studies can be the prospects to clarify the etiological role of these bacteria in the development of inflammatory bowel diseases humans and animals.


Keywords
 

Sulfate-Reducing Bacteria, Desulfovibrio piger, Sulfates, Hydrogen Sulfide, Acetate, Inflammatory Bowel Diseases, Ulcerative Colitis


Reference
 
[01]    

Barton LL and Hamilton WA (2010) Sulphate-Reducing Bacteria. Environmental and Engineered Systems. Cambridge University Press. 552 p.

[02]    

Kushkevych IV (2012) Sulfate-reducing bacteria of the human intestine. I. Dissimilatory sulfate reduction. Studia Biologica. Vol. 6(1). P. 149–180.

[03]    

Kushkevych IV (2012) Sulfate-reducing bacteria of the human intestine. II. The role in the diseases development. Studia Biologica. Vol. 6(2). P. 221–250.

[04]    

Cummings JH, Macfarlane GT, Macfarlane S (2003) Intestinal Bacteria and Ulcerative Colitis. Curr Issues Intest Microbiol. Vol. 4. P. 9–20.

[05]    

Gibson GR, Cummings JH, Macfarlane GT (1991) Growth and activities of sulphate-reducing bacteria in gut contents of health subjects and patients with ulcerative colitis. FEMS Microbiol Ecol. Vol. 86. P. 103–112.

[06]    

Gibson GR, Macfarlane GT, Cummings JH (1993) Sulphate-reducing bacteria and hydrogen metabolism in the human large intestine. Gut. Vol. 34. P. 437–439.

[07]    

Gibson GR, Macfarlane S, Macfarlane GT (1993) Metabolic interactions involving sulphate-reducing and methanogenic bacteria in the human large intestine. FEMS Microbiol Ecol. Vol. 12. P. 117–125.

[08]    

Loubinoux J, Mory F, Pereira IA, Le Faou AE (2000) Bacteremia caused by a strain of Desulfovibrio related to the provisionally named Desulfovibrio fairfieldensis. J Clin Microbiol. Vol. 38. P. 931–934.

[09]    

Loubinoux J, Bronowicji JP, Pereira IA (2002) Sulphate-reducing bacteria in human feces and their association with inflammatory diseases. FEMS Microbiol Ecol. Vol. 40. P. 107–112.

[10]    

Rowan FE, Docherty NG, Coffey JC et al. (2009) Sulphate-reducing bacteria and hydrogen sulphide in the aetiology of ulcerative colitis. British J Surgery. Vol. 96. P. 151–158.

[11]    

Pitcher MC, Cummings JH (1996) Hydrogen sulphide: a bacterial toxin in ulcerative colitis? Gut. Vol. 39. P. 1–4.

[12]    

Levine J, Ellis CJ, Furne JK, Springfield J, Levitt MD (1998) Fecal Hydrogen Sulfide Production in Ulcerative Colitis. The American J Gastroenterol. Vol. 93(1). 83–87.

[13]    

Kushkevych IV, Moroz OM (2012) Growth of various strains of sulfate-reducing bacteria of human large intestine. Studia Biologica. Vol. 6(3). P. 115–124.

[14]    

Kushkevych IV (2013) Identification of sulfate-reducing bacteria strains of human large intestine. Studia Biologica. Vol. 7(3). P. 115–124.

[15]    

Kushkevych IV, Bartos M, Bartosova L (2014) Sequence analysis of the 16S rRNA gene of sulfate-reducing bacteria isolated from human intestine. Int J Curr Microbiol Appl Sci. Vol. 3(2). P. 239–248.

[16]    

Sutton S (2011) Measurement of Microbial Cells by Optical Density. J Valid Tech Vol. 17(1). P. 46–49.

[17]    

Kolmert A, Wikstrom P, Hallberg KB (2000) A fast and simple turbidimetric method for the determination of sulfate in sulfate-reducing bacterial cultures. J Microbiol Methods. Vol. 41. P. 179–184.

[18]    

Sugiyama M (2002) Reagent composition for measuring hydrogen sulfide and method for measuring hydrogen / U.S. Pat. 6340596 B1 USA, Int. Cl. G 01 N 33/00.

[19]    

Vlizlo VV, Fedoruk RS, Makar IA et al. (2004) Physiological and biochemical methods of researches in biology, stockbreeding and veterinary medicine. Handbook Institute of Animal Biology. Third Edition: revised and enlarged Lviv. 402 p.

[20]    

Campbell MK, Farrell SO (2008) Biochemistry. Sixth Edition. Publisher: Cengage Learning. 800 p.

[21]    

Bailey NTJ (1995) Statistical Methods in Biology. Cambridge University Press. 252 p.

[22]    

Chen PY, Popovich PM (2002) Correlation: Parametric and Nonparametric Measures. Sage University Papers Series on Quantitative Applications in the Social Sciences. 104 p.





 
  Join Us
 
  Join as Reviewer
 
  Join Editorial Board
 
share:
 
 
Submission
 
 
Membership