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American Journal of Microbiology and Biotechnology

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Isolation and Identification of Aspergillus fumigatus from Brooder Pneumonia Affected Broiler Chicken

American Journal of Microbiology and Biotechnology
Vol.5 , No. 1, Publication Date: Dec. 27, 2017, Page: 1-4

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Authors

[1]	Md Zulfekar Ali, Animal Health Research Division, Bangladesh Livestock Research Institute (BLRI), Dhaka, Bangladesh.
[2]	Shirin Sultana, Department of Livestock Services (DLS), Ministry of Fisheries and Livestock, Dhaka, Bangladesh.
[3]	Mohammad Moktader Moula, Central Poultry Laboratory, Nourish Poultry and Hatchery Ltd., Dhaka, Bangladesh.
[4]	Md. Shahjalal Sagor, Food Safety Laboratory, Bangladesh Livestock Research Institute (BLRI), Dhaka, Bangladesh.

Abstract

Brooder pneumonia is a common respiratory problem during brooding period of broiler chicken in high humid region. It is caused by fungal genus Aspergillus, specially Aspergillus fumigatus. The present study was carried out the isolation and identification of Aspergillus fumigatus from brooder pneumonia affected broiler chicken at 10 days old. The characteristic grayish colors cottony colony of A. fumigatus was isolated by culture the yellowish spherical caseous granulomatous lungs nodules in potato dextrose agar media. In histopathological examination the characteristic eosinophilic hyphae of A. fumigatus was demonstrated in the coagulative necrotic center of granulomatous nodules of lungs by routine Hematoxylin and Eosin staining procedure. Finally, the 26S gene of A. fumigatus was identified by amplification of 401-bp fragment by PCR test.

Keywords

Brooder Pneumonia, Broiler Chicken, Aspergillus fumigatus, Histopathology

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