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Vol.4 , No. 5, Publication Date: Sep. 8, 2017, Page: 61-66
Stapf. (Poaceae) Leaf Ethanol Extract Against Selected Microbes&description=This study was carried out in order to determine the phytochemical constituents and antimicrobial potential of <i>Cymbopogon citratus</i> ethanol extract. The result revealed that the leaf extract contained alkaloids, carbohydrates, tannins and flavonoids, while sterols, saponins and cardiac glycosides were not detected. Antibacterial activity by agar well diffusion method and using a standard antibiotic (ceftriaxone USP) and distilled water as controls against selected Gram positive and Gram negative strains; <i>Klebsiella pneumonia ATCC10031</i>, <i>Bacillus subtilis MCTC8230, Salmonella typhi ATCC9184</i> and <i>Staphylococcus aureus</i> and a fungus <i>Candida albican ATCC19231</i>, showed that <i>Staphylococcus aureus</i> had the highest zone of inhibition of 26.5±0.1mm of the extract followed by <i>Bacillus subtilis</i> ACTC8230 with 25.25±0.1mm, <i>Candida albican ATCC1984</i> with 23.5±0.2mm, <i>Klebsiella pneumonia ATCC10031</i> with 22.0±0.1mm while <i>Salmonella typhi ATCC9184</i> has 20.5±0.1mm as the least. These results were compared to the diameter zone of inhibition of the standard drug at p≤0.05 (one-way ANOVA). The study showed that the plant extract possessed antibacterial activity against the microbes with more potency on Gram positive bacteria strains than Gram negative strains. This study therefore, justifies the ethnomedicinal use of the plant in the treatment of typhoid fever and sexually transmitted infections caused by some of these microbes.&picture=http://www.aascit.org/aascit/decorators/img/journal/919.jpg)
| [1] | Ukwubile Cletus Anes, Department of Science Laboratory Technology, School of Science and Technology, Federal Polytechnic, Bali, Nigeria. |
| [2] | Troy Salvia Malgwi, Department of Pharmacognosy, Faculty of Pharmacy, University of Maiduguri, Maiduguri, Nigeria. |
| [3] | Musa Yusuf Dibal, Department of Pharmacognosy, Faculty of Pharmacy, University of Maiduguri, Maiduguri, Nigeria. |
| [4] | Otalu Otalu Jr., Department of Microbiology, Faculty of Natural Science, Kogi State University, Anyigba, Nigeria. |
| [5] | Aliyu Nuhu, Department of Pharmacognosy and Drug Development, Faculty of Pharmaceutical Sciences, Ahmadu Bello University, Zaria, Nigeria. |
This study was carried out in order to determine the phytochemical constituents and antimicrobial potential of Cymbopogon citratus ethanol extract. The result revealed that the leaf extract contained alkaloids, carbohydrates, tannins and flavonoids, while sterols, saponins and cardiac glycosides were not detected. Antibacterial activity by agar well diffusion method and using a standard antibiotic (ceftriaxone USP) and distilled water as controls against selected Gram positive and Gram negative strains; Klebsiella pneumonia ATCC10031, Bacillus subtilis MCTC8230, Salmonella typhi ATCC9184 and Staphylococcus aureus and a fungus Candida albican ATCC19231, showed that Staphylococcus aureus had the highest zone of inhibition of 26.5±0.1mm of the extract followed by Bacillus subtilis ACTC8230 with 25.25±0.1mm, Candida albican ATCC1984 with 23.5±0.2mm, Klebsiella pneumonia ATCC10031 with 22.0±0.1mm while Salmonella typhi ATCC9184 has 20.5±0.1mm as the least. These results were compared to the diameter zone of inhibition of the standard drug at p≤0.05 (one-way ANOVA). The study showed that the plant extract possessed antibacterial activity against the microbes with more potency on Gram positive bacteria strains than Gram negative strains. This study therefore, justifies the ethnomedicinal use of the plant in the treatment of typhoid fever and sexually transmitted infections caused by some of these microbes.
Keywords
Phytochemical, Antimicrobial, Cymbopogon citratus, Bacteria Strains
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