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Vol.4 , No. 6, Publication Date: Oct. 13, 2017, Page: 75-82
 and 18S rRNA sequences of the ribosomal DNA and compared these sequences to species from other countries. Eight <i>T. evansi</i> isolates were characterized genetically by PCR amplification of ITS-1 rDNA with the length of 290 – 900 bp, and sequencing of ITS-2 rDNA with the length of 164 – 167 bp, and 18S rRNA with the length of 741 –804 bp. Basic Local Alignment Search Tool data of the obtained ITS-2 sequences revealed that they corresponded to those of <i>T. evansi</i>, Iranian camel isolate (JN896755.1) with the homology of 97%. The nucleotide sequence variation of 18S rRNA region between Sudanese <i>T. evani</i> isolates was 1%. The mean GC content of 18S rRNA sequences was 10.77%, and that of ITS-2 rDNA sequences was 7.87%. In contrast to the ITS-2 region, multiple alignment of the nucleotide sequence of the 18S rRNA showed a high degree of sequence conservation in various <i>T. evansi</i> isolates. <i>T. evansi</i> separated in to two clades when subjected to phylogenetic analysis. Sudanese isolates of <i>T. evansi</i> 18S rRNA sequences clustered tightly within the <i>T. evansi</i> Kenyan isolate clade. Phylogenetic tree generated by 18S rRNA was unable to clearly show inter-and intraspecific genetic diversity of <i>T. evansi</i> isolates. The phylogenetic tree inferred from the ITS-2 nucleotide sequences clearly showed the genetic diversity of the Sudanese isolates. The data represented here could be applied to parasite dynamics and epidemiological studies as well as prevention and control of “Surra”. The sequences of <i>T. evansi</i> 18S rRNA have been deposited in the Gen Bank (Accession numbers: MF142282 – MF142289).&picture=http://www.aascit.org/aascit/decorators/img/journal/919.jpg)
| [1] | Hamid Ibrahim Mohamed Nour Croof, Ministry of Animals Resources, Gedaref, Sudan. |
| [2] | Imna Malelle, Tanzania Veterinary Laboratory Agency, Vector Borne Disease Institute, Tanga, Tanzania. |
| [3] | Hamis Said Nyingilili, School of Environment and Life Sciences, University of Salford, Salford, United Kingdom. |
| [4] | Sonia Sadeq, School of Environment and Life Sciences, University of Salford, Salford, United Kingdom. |
| [5] | Darren Brooks, School of Environment and Life Sciences, University of Salford, Salford, United Kingdom. |
| [6] | Nahla Osman Mohamed Ali, Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, Khartoum North, Sudan. |
To a better understanding of the genetics of cameline Trypanosomosis “Surra”, the genetic diversity of Trypanosoma evansi in naturally infected Sudanese camel was assessed using internal transcribed spacer 1 and 2 (ITS-1 and ITS-2) and 18S rRNA sequences of the ribosomal DNA and compared these sequences to species from other countries. Eight T. evansi isolates were characterized genetically by PCR amplification of ITS-1 rDNA with the length of 290 – 900 bp, and sequencing of ITS-2 rDNA with the length of 164 – 167 bp, and 18S rRNA with the length of 741 –804 bp. Basic Local Alignment Search Tool data of the obtained ITS-2 sequences revealed that they corresponded to those of T. evansi, Iranian camel isolate (JN896755.1) with the homology of 97%. The nucleotide sequence variation of 18S rRNA region between Sudanese T. evani isolates was 1%. The mean GC content of 18S rRNA sequences was 10.77%, and that of ITS-2 rDNA sequences was 7.87%. In contrast to the ITS-2 region, multiple alignment of the nucleotide sequence of the 18S rRNA showed a high degree of sequence conservation in various T. evansi isolates. T. evansi separated in to two clades when subjected to phylogenetic analysis. Sudanese isolates of T. evansi 18S rRNA sequences clustered tightly within the T. evansi Kenyan isolate clade. Phylogenetic tree generated by 18S rRNA was unable to clearly show inter-and intraspecific genetic diversity of T. evansi isolates. The phylogenetic tree inferred from the ITS-2 nucleotide sequences clearly showed the genetic diversity of the Sudanese isolates. The data represented here could be applied to parasite dynamics and epidemiological studies as well as prevention and control of “Surra”. The sequences of T. evansi 18S rRNA have been deposited in the Gen Bank (Accession numbers: MF142282 – MF142289).
Keywords
Camel, Trypanosoma evansi, Phylogenetics, Ribosomal DNA Sequences, Sudan
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