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ISSN: 2375-3005

American Journal of Microbiology and Biotechnology

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Molecular Isolation and Characterization of Trypanosoma evansi in Dromedary Camels from Different Regions of Sudan

American Journal of Microbiology and Biotechnology
Vol.4 , No. 6, Publication Date: Oct. 13, 2017, Page: 67-74

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Authors

[1]	Hamid Ibrahim Mohamed Nour Croof, Ministry of Animals Resources, Gedaref, Sudan.
[2]	Imna Malelle, Tanzania Veterinary Laboratory Agency, Vector & Vector Borne Disease Institute, Tanga, Tanzania.
[3]	Darren Brooks, School of Environment and Life Sciences, University of Salford, Salford, United Kingdom.
[4]	Hamid Suliman Abdella, Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, Khartoum North, Sudan.
[5]	Nahla Osman Mohamed Ali, Department of Parasitology, Faculty of Veterinary Medicine, University of Khartoum, Khartoum North, Sudan.

Abstract

Trypanosoma evansi is classified under the subgenus Trypanozoon along with the T. brucei spp. and T. equiperdumi, and they are main causative agents of Animal trypanosomoses throughout the world. T. evansi causes Surra via mechanical transmission in Camels. Whole blood samples were collected from 102 suspected camels from 18 different geographic regions in Sudan. T. evansi-affected camels were detected by Wet smear method, and the positive blood samples (10 out of 102) were subjected to DNA extraction and TBR-PCR examination and phylogenetic analysis. Basic Local Alignment Search Tool data of the obtained TBR sequences revealed that partial sequence of most of them corresponded to those of T. evansi (VSG), Malaysian isolate selanger 2 (AM497934.1) with the homology of 100%. The phylogenetic tree inferred from the TBR nucleotide sequences (137bp) clearly showed the genetic diversity of the parasites. Phylogenetic and molecular analyses of this region of variable surface glycoprotein (VSG) showed that three distinct genotypes of T. evansi in Sudanese dromedary camels are present. This study suggests that T. evansi could be a polyphyltic group with 4 clades or a monophlytic group and all isolates have a common ancestor. More informative genetic marker is required in order to have ultimate conclusion.

Keywords

Trypanosoma evansi, Molecular, Characterization, Camels, Phylogeny, Sudan

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