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American Journal of Microbiology and Biotechnology

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Extraction and Optimization of L-asparaginase Produced by Local Isolates of E.coli

American Journal of Microbiology and Biotechnology
Vol.5 , No. 1, Publication Date: Feb. 27, 2018, Page: 5-11

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Authors

[1]	Hanan Moawia Ibrahim, Department of Biology, Central Lab., Ministry of Higher Education and Scientific Research, Khartoum, Sudan.
[2]	Safeya Mohammed Shareef Mohammed Salih, Department of Biology, Central Lab., Ministry of Higher Education and Scientific Research, Khartoum, Sudan.
[3]	Runda Eltayeb, Department of Biology, Central Lab., Ministry of Higher Education and Scientific Research, Khartoum, Sudan.
[4]	Elrashied Elimam. Elkhidir, College of Agricultural Economic, Faculty of Agriculture, University of Khartoum, Khartoum, Sudan.

Abstract

This study investigated the production of the anticancer enzyme L-asparaginase by the local isolate of E.coli. Sewage water samples were collected from different locations in Sudan. 96% out of The 180 samples collected were found to be positive E.coli while 4% were negative in EMB that showed pink color around the colony. 42% of the positive isolates were L-asparaginase producer with inhibition zone ranged from (15-40 mm) while 58% of The isolates not L-asparginase producer. These isolates were identified microscopically, biochemically and confirmed by molecular characterization. The optimization of the physical conditions in term of temperature and pH showed that the optimum pH is of 9.0 and optimum temperature is of 25°C using statistical experimental design, Response Surface Methodology (RSM) revealed in higher L-asparaginase yield of 2.2U/ml/min.

Keywords

Anti-cancerous Enzyme, Optimization, L-asparaginase, E.coli, Response Surface Methodology (RSM)

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