ISSN: 2375-3005
American Journal of Microbiology and Biotechnology  
Manuscript Information
 
 
Optimization of Medium Components for Improving the Antifungal Activity from Pseudomonas protegens XL03
American Journal of Microbiology and Biotechnology
Vol.3 , No. 4, Publication Date: Jul. 29, 2016, Page: 29-35
2941 Views Since July 29, 2016, 1194 Downloads Since Jul. 29, 2016
 
 
Authors
 
[1]    

Feifei Wang, Institute of Hydrobiology, Jinan University, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Guangzhou, People’s Republic of China.

[2]    

Qizhong Zhang, Institute of Hydrobiology, Jinan University, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Guangzhou, People’s Republic of China.

 
Abstract
 

Pseudomonas protegens XL03 showed the antifungal activity against Saprolegnia sp., and its cell-free disruption supernatant (CFDS) inhibited the hyphal growth with an antifungal zone diameter of 19.34±1.20 mm. In order to improve the production of the antifungal compounds which resided in intracellular, Plackett-Burman design (PBD) and Response surface methodology (RSM) were applied to optimize the medium of the strain XL03. Firstly, the process parameters that significantly affected the antifungal compounds production (tryptone, glucose and NaCl) were identified by PBD; Subsequently, the optimum levels of these significant parameters were determined using RSM, which showed as follows: tryptone (7.50 g/L), glucose (8.79 g/L) and NaCl (1.27 g/L); Finally, the experimental validation was conducted under the optimal conditions,and the antifungal activity (antifungal zone diameter) increased about 4 mm in comparison with that on the un-optimized medium.


Keywords
 

Pseudomonas sp., Saprolegnia sp., Antifungal Activity, Optimized, PBD, RSM


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