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Vol.3 , No. 4, Publication Date: Jul. 29, 2016, Page: 29-35
 inhibited the hyphal growth with an antifungal zone diameter of 19.34±1.20 mm. In order to improve the production of the antifungal compounds which resided in intracellular, Plackett-Burman design (PBD) and Response surface methodology (RSM) were applied to optimize the medium of the strain XL03. Firstly, the process parameters that significantly affected the antifungal compounds production (tryptone, glucose and NaCl) were identified by PBD; Subsequently, the optimum levels of these significant parameters were determined using RSM, which showed as follows: tryptone (7.50 g/L), glucose (8.79 g/L) and NaCl (1.27 g/L); Finally, the experimental validation was conducted under the optimal conditions，and the antifungal activity (antifungal zone diameter) increased about 4 mm in comparison with that on the un-optimized medium.&picture=http://www.aascit.org/aascit/decorators/img/journal/919.jpg)
| [1] | Feifei Wang, Institute of Hydrobiology, Jinan University, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Guangzhou, People’s Republic of China. |
| [2] | Qizhong Zhang, Institute of Hydrobiology, Jinan University, Engineering Research Center of Tropical and Subtropical Aquatic Ecological Engineering Ministry of Education, Eutrophication and Red Tide Prevention of Guangdong Higher Education Institutes, Guangzhou, People’s Republic of China. |
Pseudomonas protegens XL03 showed the antifungal activity against Saprolegnia sp., and its cell-free disruption supernatant (CFDS) inhibited the hyphal growth with an antifungal zone diameter of 19.34±1.20 mm. In order to improve the production of the antifungal compounds which resided in intracellular, Plackett-Burman design (PBD) and Response surface methodology (RSM) were applied to optimize the medium of the strain XL03. Firstly, the process parameters that significantly affected the antifungal compounds production (tryptone, glucose and NaCl) were identified by PBD; Subsequently, the optimum levels of these significant parameters were determined using RSM, which showed as follows: tryptone (7.50 g/L), glucose (8.79 g/L) and NaCl (1.27 g/L); Finally, the experimental validation was conducted under the optimal conditions，and the antifungal activity (antifungal zone diameter) increased about 4 mm in comparison with that on the un-optimized medium.
Keywords
Pseudomonas sp., Saprolegnia sp., Antifungal Activity, Optimized, PBD, RSM
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