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American Journal of Microbiology and Biotechnology

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Study of Biofilm Production and Antimicrobial Resistance Pattern of Klebsiella Pneumoniae Isolated from Urinary Catheter at the University Hospital of Tlemcen

American Journal of Microbiology and Biotechnology
Vol.3 , No. 2, Publication Date: Mar. 28, 2016, Page: 13-17

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Authors

[1]	Samia Bellifa, Laboratory of Food, Biomedical and Environmental Microbiology (LAMAABE), University of Tlemcen, Tlemcen, Algeria.
[2]	Hafida Hassaine, Laboratory of Food, Biomedical and Environmental Microbiology (LAMAABE), University of Tlemcen, Tlemcen, Algeria.
[3]	Ibtissem Kara Terki, Laboratory of Food, Biomedical and Environmental Microbiology (LAMAABE), University of Tlemcen, Tlemcen, Algeria.
[4]	Wafae Didi, Laboratory of Food, Biomedical and Environmental Microbiology (LAMAABE), University of Tlemcen, Tlemcen, Algeria.
[5]	Imene M’hamedi, Laboratory of Food, Biomedical and Environmental Microbiology (LAMAABE), University of Tlemcen, Tlemcen, Algeria.
[6]	Meriem Lachachi, Laboratory of Food, Biomedical and Environmental Microbiology (LAMAABE), University of Tlemcen, Tlemcen, Algeria.
[7]	Ibrahim Benamar, Laboratory of Food, Biomedical and Environmental Microbiology (LAMAABE), University of Tlemcen, Tlemcen, Algeria.
[8]	Touhami Morghad, Laboratory of Food, Biomedical and Environmental Microbiology (LAMAABE), University of Tlemcen, Tlemcen, Algeria.
[9]	Sarah Gaouar, Laboratory of Food, Biomedical and Environmental Microbiology (LAMAABE), University of Tlemcen, Tlemcen, Algeria.

Abstract

Klebsiella pneumoniae is an important gram-negative opportunistic pathogen causing primarily urinary tract infections, respiratory infections, and bacteraemia. The ability of bacteria to form biofilms on medical devices, e.g. catheters, has a major role in development of many nosocomial infections. There are various methods to detect biofilm production like Tissue Culture Plate (TCP), Tube method (TM), Congo Red Agar method (CRA), bioluminescent assay, piezoelectric sensors, and fluorescent microscopic examination. In the present study we screened 100 strains of K. pneumoniae isolated from urinary catheter at the university hospital of Tlemcen (Algeria) by three methods for the detection of biofilms (TCP, TM, CRA). Antibiotic susceptibility test of biofilm producing bacteria was performed by using the Kirby-Bauer disc diffusion technique according to CLSI guidelines. The TCP method was considered to be superior to TM and CRA. From the total of 100 clinical isolates, TCP method detected 69% as high, 21% moderate and 10% as weak or non-biofilm producers. We have observed higher antibiotic resistance in biofilm producing bacteria than non-biofilm producers. This study demonstrates a high propensity among the clinical isolates of K. pneumoniae to form biofilm and a significant association of biofilm with multiple drug resistance.

Keywords

Biofilm, Klebsiella pneumoniae, TCP, TM, RCA

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