AASCIT - Journal - All Issues

ISSN: 2375-3005

American Journal of Microbiology and Biotechnology

The Journal

Manuscript Information

Elaboration of Asparagus officinalis L. by in vitro and Hydroponic Combined Method

American Journal of Microbiology and Biotechnology
Vol.2 , No. 6, Publication Date: Feb. 15, 2016, Page: 92-96

1575 Views Since February 15, 2016, 1183 Downloads Since Feb. 15, 2016

Paper in PDF (3546K)

Follow on

Authors

[1]	Elmira Sargsyan, G. S. Davtyan Institute of Hydroponic Problems, National Academy of Sciences Republic of Armenia, Laboratory of “Tissue Culture”, Yerevan, Armenia.
[2]	Anush Vardanyan, G. S. Davtyan Institute of Hydroponic Problems, National Academy of Sciences Republic of Armenia, Laboratory of “Tissue Culture”, Yerevan, Armenia.
[3]	Ali Parsaeimehr, Institute of Biotechnology, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
[4]	Ani Bekyan, G. S. Davtyan Institute of Hydroponic Problems, National Academy of Sciences Republic of Armenia, Laboratory of “Tissue Culture”, Yerevan, Armenia.

Abstract

Main objective of the research is cultivation and propagation of valuable medicinal plant Aspatagus officinalis L. (Mary Washington) by hydroponics and in vitro combined method. From seeds of hydroponic plants were obtained in vitro plantlets. To obtain callus tissues various explants (hypocotyl, root, apical bud, stem segment) were separated from micro-plantlets. In vitro callus cultures were obtained. Biosynthetic possibility and activity of callus tissues in case of various growth regulators and concentrations were determined. All tested explants on MS nutrient medium with hormones (BAP - 1.0 mg/l, 2.4D - 2.0 mg/l and BAP - 1.0 mg/l, 2.4D - 0.5 mg/l, α-NAA - 0.5 mg/l) inducted callus tissues. The replanting of callus was implemented once in a month, on the fresh nutrient medium. Viable callus cultures were obtained and used for induction of rhizogenesis and organogenesis. On MS medium optimum result was in case of callus from hypocotyl explant, with BAP - 1.0 mg/l, 2.4D - 0.5 mg/l and α-NAA - 0.5 mg/l or Kinetin - 1 mg/l concentration, which provided regeneration of 15 - 20 adventive shoots. Shoot-regenerants multiplicated micro-clonaly. Among tested phytohormones the best result was 0.5 mg/l IBA concentration, providing 62% rhizogenesis of shoot-regenetants. In spring, the rooted in vitro micro-plantlets after acclimatization were planted in the open air gravel hydroponic conditions for propagation.

Keywords

Asparagus Officinalis L., In Vitro, Callus Culture, Clonal Micropropagation, Adventive Shoot

Reference

[01]	Bousserouel S., Le Grandois J., Gosse F., Werner D., Barth S.W., Marchioni E., Marescaux J., Raul F.A. (2013) Methanolic extract of white Asparagus shoots activates trail apoptotic death pathway in human cancer cells and inhibits colon carcinogenesis in a preclinical model. Int. J. of Oncology, 43 (2), p. 394–404.
[02]	Butenko R. G. (1999) The Biology of cultivated cells of higher plants in vitro and biotechnology on its base. Monograph, Moscow, FAC-Press, p.159 (In Russian).
[03]	Huang X., Kong L. (2006) Steroidal saponins from roots of Asparagus officinalis. Steroids, V.71, Issue 2, p. 171–176.
[04]	Huang X.F., Lin Y.Y., Kong L.Y. (2008) Steroids from the roots of Asparagus officinalis and their cytotoxic activity. J. Integr. Plant Biol. 50, p.717–722.
[05]	Jang D.S., Cuendet M., Fong H.H., Pezzuto J.M., Kinghorn A.D. (2004) Constituents of Asparagus officinalis evaluated for inhibitory activity against cyclooxygenase-2. J. Agric. Food Chem., 52, p. 2218–2222.
[06]	Khorasani A., Sani W., Philip K., Mat Taha R., Ra A. (2010) Antioxidant and antibacterial activities of ethanolic extracts of Asparagus officinalis cv. Mary Washington: Comparison of in vivo and in vitro grown plant bioactivities. African J. of Biotechnology, V. 9 (49), p. 8460-8466.
[07]	Lebeda A.F., Drujenko N.I., Isayko A.P., Sobko V.G. (2005) Medicinal plants. p.722 (In Russian).
[08]	Murashige T., Skoog F.A. (1962) Revised Medium for Rapid Growing and Bioassay with Tobacco Tissue Culture. Physical Plantarium, V. 1 (15), p. 473-497.
[09]	Negi J.S., Singh P., Joshi G.P., Rawat M.S., Bisht V.K. (2010) Chemical constituents of Asparagus. Pharmacognosy Review, 4(8), p. 215–220.
[10]	Petrus-Vancea A. (2011) New techniques in Asparagus officinalis L. micro-propagation. /Scientific Papers, UASVM Bucharest, Series - A, V. L IV, p. 431-438.
[11]	Rodriguez R., Jaramillo S., Rodriguez G., Espejo J.A., Guillen R., Fernandez-Bolanos J., Heredia A., Jimenez A. (2005) Antioxidant activity of ethanolic extracts from several asparagus cultivars. J. of Agric. Food Chemistry, V.53, p. 5212–5217.
[12]	Shao Y., Chin C.K., Ho C.T., Ma W., Garrison S.A., Huang M.T. (1996) Anti-tumor activity of the crude saponins obtained from asparagus. Cancer Lett., V.104, p. 31–36.
[13]	Stajner N. (2013) Micropropagation of Asparagus by in vitro shoot culture. Methods Mol. Biol., p. 341-351, http://www.ncbi.nlm.nih.gov/pubmed/23179712.
[14]	Wang H., Ng T.B. (2001) Isolation of a novel deoxyribonuclease with antifungal activity from Asparagus officinalis seeds. Biochem. Biophys. Res. Commun., V. 289, p. 120–124.
[15]	Wang M., Tadmor Y., Wu Q.L., Chin C.K., Garrison S.A., Simon J.E. (2003) Quantification of protodioscin and rutin in asparagus shoots by LC/MS and HPLC methods. J. of Agric. Food Chemistry, V. 51, p. 6132–6136.
[16]	Yamamori A., Onodera S., Kikuchi M., Shiomi N. (2002) Two novel oligosaccharides formed by 1F-fructosyltransferase purified from roots of asparagus (Asparagus officinalis L.). Bioscience Biotechnology Biochemistry, V. 66, p. 1419–1422.