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Vol.2 , No. 5, Publication Date: Aug. 20, 2015, Page: 64-68
 and Its Role in Cellulose Synthesis in Arabidopsis Thaliana&description=In this study, we subcloned the full-length cDNA of the UDP-glucose pyrophosphorylase gene of Corchorus capsularis into expression vector pCAMBIA 1301. Arabidopsis were transformed by floral-dip method, PCR and southern hybridization results indicate exogenous gene was integrated into the Arabidopsis genome. Overexpression of CcUGPase gene revealed increased height and more rapid growth rate in transgenic Arabidopsis compared with control lines. Importantly, the transgenic Arabidopsis have more cellulose content than control lines, while the lignin content remained unchanged. The results indicate that jute UGPase gene participates in cellulose biosynthesis in plants, which provides an important basis for the application of the UGPase gene in the improvement of jute fiber quality.&picture=http://www.aascit.org/aascit/decorators/img/journal/919.jpg)
| [1] | Gaoyang Zhang, College of Life Sciences, Shangrao Normal University, Shangrao, P. R. China. |
| [2] | Jielou Deng, College of Life Sciences, Shangrao Normal University, Shangrao, P. R. China. |
| [3] | Jiantang Xu, Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou, P. R. China. |
| [4] | Jianmin Qi, Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou, P. R. China. |
In this study, we subcloned the full-length cDNA of the UDP-glucose pyrophosphorylase gene of Corchorus capsularis into expression vector pCAMBIA 1301. Arabidopsis were transformed by floral-dip method, PCR and southern hybridization results indicate exogenous gene was integrated into the Arabidopsis genome. Overexpression of CcUGPase gene revealed increased height and more rapid growth rate in transgenic Arabidopsis compared with control lines. Importantly, the transgenic Arabidopsis have more cellulose content than control lines, while the lignin content remained unchanged. The results indicate that jute UGPase gene participates in cellulose biosynthesis in plants, which provides an important basis for the application of the UGPase gene in the improvement of jute fiber quality.
Keywords
CcUGPase, Jute, Cellulose, Southern Hybridization, A. thaliana, Quality
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