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Vol.2 , No. 5, Publication Date: Aug. 20, 2015, Page: 69-74
 were included in this study. All isolates were identified and confirmed by standard methods. Isolates were screened for β- lactamase and extended spectrum beta- lactamases (ESBLs) using Nitrocefin test and Double disk synergy detection test. All confirmed β-lactamases producing isolates were tested for susceptibility to ten different antibiotics by disk diffusion method. The overall prevalence of 51(45.1%) and 33(27.2%) were recorded for β-lactamases and ESBLs production respectively. E.coli showed higher prevalence (47%, 30.3%) followed by <i>Klebseilla</i> species (42.7%, 27.7%) for β-lactamase and ESBLs respectively. Age distribution of β-lactamse producing isolates among patients showed highest prevalence (68.4%) among 41-50 years age group. ESBL producers were 50.0% of the organisms isolated from individuals 51years and above. Both β- lactamase and ESBLs producers were susceptible to Imipenem and Nitrofuration and resistant to Ampicilin, Augmentin and the cephalosporins. This study indicates that β-lactamase producers are widely distributed in the study area. This poses public health challenges.&picture=http://www.aascit.org/aascit/decorators/img/journal/919.jpg)
| [1] | Eze E. A., Department of Microbiology, University of Nigeria, Nsukka, Nigeria. |
| [2] | Agbo E. C., Department of Microbiology, University of Nigeria, Nsukka, Nigeria. |
| [3] | Eze C. N., Department of Microbiology, University of Nigeria, Nsukka, Nigeria. |
Infections due to β-Lactamases producing Escherichia coliand Klebsiella species are increasingly recognized in recent years. They are creating clinical concerns. This is because few antibiotics are available as therapeutic options. A total of 113 screened isolates of E.coli and Klebsiella spp obtained from November 2013 to December 2014 from various clinical specimens such as urine, stool, sputum, wound swab and high vaginal swab (HVS) were included in this study. All isolates were identified and confirmed by standard methods. Isolates were screened for β- lactamase and extended spectrum beta- lactamases (ESBLs) using Nitrocefin test and Double disk synergy detection test. All confirmed β-lactamases producing isolates were tested for susceptibility to ten different antibiotics by disk diffusion method. The overall prevalence of 51(45.1%) and 33(27.2%) were recorded for β-lactamases and ESBLs production respectively. E.coli showed higher prevalence (47%, 30.3%) followed by Klebseilla species (42.7%, 27.7%) for β-lactamase and ESBLs respectively. Age distribution of β-lactamse producing isolates among patients showed highest prevalence (68.4%) among 41-50 years age group. ESBL producers were 50.0% of the organisms isolated from individuals 51years and above. Both β- lactamase and ESBLs producers were susceptible to Imipenem and Nitrofuration and resistant to Ampicilin, Augmentin and the cephalosporins. This study indicates that β-lactamase producers are widely distributed in the study area. This poses public health challenges.
Keywords
Prevalence, Eschericia coli, Klebsiella spp, β- lactamase Producers, ESBLs Producers, Antibiotics Susceptibility
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