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Vol.2 , No. 4, Publication Date: Jul. 29, 2015, Page: 57-63
 were antigenically validated to be proposed as candidate antigens to serologically diagnose American cutaneous leishmaniasis (ACL). Validation was carried out in 140 sera-samples, 71 from patients suffering active ACL previously diagnosed using clinical, parasitological, serological and molecular (Polymerase chain reaction-PCR assay) methods, and 69 from individuals either healthy or suffering from other etiologies in order to estimate its sensitivity and specificity, respectively. The use of the L. braziliensis-GPI-AMP antigen in western blot assays revealed 99% (70/71) sensitivity to detect circulating anti-Leishmania antibodies in sera-samples of the tested patients. The positive reactivity was evidenced by a constant band of 50 kDa molecular mass detected in all the patients suffering ACL. A 100% specificity of the L. braziliensis-GPI-AMP antigen as diagnostic tool was recorded after confirming negative results in all control samples. The obtained values of sensitivity (99%), specificity (100%), positive predictive value (1.00) and negative predictive value (0.99) demonstrated the reliability of the L. braziliensis-GPI-AMP antigen to sero-diagnose ACL. In addition, the constant expression of the 50kDa band, led us to consider it as a biochemical marker capable of producing reliable, sensitive and specific signal to detect active ACL.&picture=http://www.aascit.org/aascit/decorators/img/journal/919.jpg)
| [1] | Agustina Rojas, Department of Biology, Faculty of Sciences, Centre for Parasitological Research “J. F. Torrealba”, University of Los Andes, Merida, Venezuela. |
| [2] | Gladys Crisante, Department of Biology, Faculty of Sciences, Centre for Parasitological Research “J. F. Torrealba”, University of Los Andes, Merida, Venezuela. |
| [3] | Pablo Garcia, Laboratory of Biotechnology of Microorganisms (BIOMI), Faculty of Sciences, University of Los Andes, Merida, Venezuela. |
| [4] | José Carrero, Ministry of Health, Dermatological Institute, Tovar-Merida, Venezuela. |
| [5] | Néstor Añez, Department of Biology, Faculty of Sciences, Centre for Parasitological Research “J. F. Torrealba”, University of Los Andes, Merida, Venezuela. |
Purified Leishmania braziliensis-GPI-anchored membrane proteins (L. braziliensis-GPI-AMP) were antigenically validated to be proposed as candidate antigens to serologically diagnose American cutaneous leishmaniasis (ACL). Validation was carried out in 140 sera-samples, 71 from patients suffering active ACL previously diagnosed using clinical, parasitological, serological and molecular (Polymerase chain reaction-PCR assay) methods, and 69 from individuals either healthy or suffering from other etiologies in order to estimate its sensitivity and specificity, respectively. The use of the L. braziliensis-GPI-AMP antigen in western blot assays revealed 99% (70/71) sensitivity to detect circulating anti-Leishmania antibodies in sera-samples of the tested patients. The positive reactivity was evidenced by a constant band of 50 kDa molecular mass detected in all the patients suffering ACL. A 100% specificity of the L. braziliensis-GPI-AMP antigen as diagnostic tool was recorded after confirming negative results in all control samples. The obtained values of sensitivity (99%), specificity (100%), positive predictive value (1.00) and negative predictive value (0.99) demonstrated the reliability of the L. braziliensis-GPI-AMP antigen to sero-diagnose ACL. In addition, the constant expression of the 50kDa band, led us to consider it as a biochemical marker capable of producing reliable, sensitive and specific signal to detect active ACL.
Keywords
Leishmaniasis, Diagnosis, GPI-Anchored Proteins, Leishmania braziliensis
Reference
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