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Vol.2 , No. 3, Publication Date: May 13, 2015, Page: 38-43
 inside the body of the patient or vice versa, are susceptible to be altered by Candida spp. and promote the formation of biofilms which consolidates the risk of invasive nosocomial infections; i.e., these structures are considered as a nest for disease because it is not easily eradicate by conventional antifungal therapy. Such as the diagnosis of candidiasis related to catheter is difficult, the differentiation between catheter infection and a simple contamination is essential to establish an antifungal treatment. This study aimed adapts to yeasts the Brun-Buisson (1987) method which only concerned by bacteria, it’s why we conducted our study between February 2011 and January 2012 at the Hospital University Center of Sidi Bel Abbès-Algeria that aims to evaluate the various types of fungal catheters infectivities (contaminations, colonization and infections) and their corresponding rates, as well as the responsible yeast species. At the end, the ability to form biofilms was checked. The results showed that three types of fungal infectivities of catheters were identified. On the other hand, SEM images showed clearly Candida biofilms on the surfaces of catheters.&picture=http://www.aascit.org/aascit/decorators/img/journal/919.jpg)
| [1] | Sidi Mohammed Lahbib Seddiki, Laboratory Antifungal Antibiotic, Physico-chemical synthesis and biological activity, University of Tlemcen, Imama, Algeria; University Center of Naâma, Institute of Sciences, Dept. of nature and life sciences, Naâma, Algeria. |
| [2] | Zahia Boucherit-Otmani, Laboratory Antifungal Antibiotic, Physico-chemical synthesis and biological activity, University of Tlemcen, Imama, Algeria. |
| [3] | Reda Ali Bettioui, Laboratory: Actions of the human for the protection of the environment and application in public health, department of ecology and environment, University of Tlemcen, Imama, Algeria. |
| [4] | Kebir Boucherit, University Center of Naâma, Institute of Sciences, Dept. of nature and life sciences, Naâma, Algeria. |
| [5] | Mourad Taleb, Department of Epidemiology and preventive medicine, University Hospital of Sidi Bel Abbes, Algeria. |
| [6] | Dennis Kunkel, Dennis Kunkel Microscopy Inc, Kailua, USA. |
The invasive nosocomial infections due to Candida species are responsible for increasing the length of stay, cost of hospitalization and morbidity in immunocompromised patients. Their severity and rapid progressivity are owed to the difficulty of diagnosis. Various catheters, which are often used to train a body fluid (blood, urine, infusion, parenteral nutrition, medication ...) inside the body of the patient or vice versa, are susceptible to be altered by Candida spp. and promote the formation of biofilms which consolidates the risk of invasive nosocomial infections; i.e., these structures are considered as a nest for disease because it is not easily eradicate by conventional antifungal therapy. Such as the diagnosis of candidiasis related to catheter is difficult, the differentiation between catheter infection and a simple contamination is essential to establish an antifungal treatment. This study aimed adapts to yeasts the Brun-Buisson (1987) method which only concerned by bacteria, it’s why we conducted our study between February 2011 and January 2012 at the Hospital University Center of Sidi Bel Abbès-Algeria that aims to evaluate the various types of fungal catheters infectivities (contaminations, colonization and infections) and their corresponding rates, as well as the responsible yeast species. At the end, the ability to form biofilms was checked. The results showed that three types of fungal infectivities of catheters were identified. On the other hand, SEM images showed clearly Candida biofilms on the surfaces of catheters.
Keywords
Candida Spp, Catheters, Fungal Infectivity, Biofilms, SEM
Reference
| [01] | Ascioglu S, Rex JH, de Pauw B, et al. Defining Opportunistic Invasive Fungal Infections in Immunocompromised Patients with Cancer and Hematopoietic Stem Cell Transplants: An International Consensus. Clin Infect Dis 2002; 34 : 7-14. |
| [02] | Alangaden GJ. Nosocomial Fungal Infections: Epidemiology, Infection Control, and Prevention. Infect Dis Clin North Am 2011; 25: 201–225. |
| [03] | Falagas ME, Roussos N, Vardakas KZ. Relative frequency of albicans and the various non-albicans Candida spp among candidemia isolates from inpatients in various parts of the world: a systematic review. Int J Infect Dis 2010; 14 : e954-66. |
| [04] | Boo TW, O’Reilly B, O’Leary J, Cryan B. Candidaemia in an Irish tertiary referral hospital: Epidemiology and prognostic factors. Mycoses 2005; 48 : 251 –259. |
| [05] | Ramage G, Mowat E, Jones B, Williams C, Lopez-Ribot J. Our Current Understanding of Fungal Biofilms. Crit Rev Microbiol 2009; 35 : 340-55. |
| [06] | Alem MA, Oteef MD, Flowers TH, Douglas LJ. Production of tyrosol by Candida albicans biofilms and its role in quorum sensing and biofilm development. Eukaryot Cell 2006; 5 : 1770-1779. |
| [07] | Rodney MD. Biofilm Elimination on Intravascular Catheters: Important Considerations for the Infectious Disease Practitioner. Clin Infect Dis 2011; 52 : 1038-1045. |
| [08] | Hasan F, Xess I, Wang X, Jain N, Fries BC. Biofilm formation in clinical Candida isolates and its association with virulence. Microbes Infect 2009; 11 : 753–761. |
| [09] | Silva S, Henriques M, Martins A, Oliveira R, Williams D, Azeredo J. Biofilms of non-Candida albicans Candida species: quantification, structure and matrix composition. Med Mycol 2009; 47: 681-689. |
| [10] | Boucherit-Atmani Z, Seddiki SML, Boucherit K, Sari-Belkharoubi L, Kunkel D. Candida albicans biofilms formed into catheters and probes and their resistance to amphotericin B. J Mycol Med 2011; 21 : 182-7. |
| [11] | Gouin F, Vaggiano M. Candidoses systémiques : épidémiologie et diagnostic. Réan. Urg 1996; 5: 7-11. |
| [12] | Saiman L, Ludington E, Dawson JD, et al. Risk factors for Candida species colonization of neonatal intensive care unit patients. Pediatr Infect Dis J 2001; 20 : 1119-1124. |
| [13] | Douglas LJ. Candida biofilms and their role in infection. Trends Microbiol 2003; 11: 30-36. |
| [14] | Hot A, Mittaine B, Dupont B. Infections fongiques invasives du grand prématuré. J Mycol Med 2007; 17: 33-41. |
| [15] | Rex JH, Walsh TJ, Sobel JD, et al. Practice guidelines for the treatment of candidiasis. Infectious Diseases Society of America. Clin Infect Dis 2000; 30 : 662–78. |
| [16] | Brun-buisson C. analyse critique des méthodes diagnostiques d'infection liée au cathéter sur matériel enlèvent. Réan. Urg 1994; 3 : 343-46. |
| [17] | Carrière C, Marchandin H. Infections liées aux cathéters veineux centraux : diagnostic et définitions. Néphrologie 2001; 22 : 433-37. |
| [18] | Veyssier P, Domart Y, Liebbe AM (Collection Abrégés de Médecine). Infections Nosocomiales, 2nded, Paris, 1998. |
| [19] | Maki DG, jarretf F, sarafin HW. A semi-quantitative culture method for identification of catheter-related infection in the burn patient. J. Surg. Res 1977; 22: 513-520. |
| [20] | Brun-Buisson C, Abrouk F, Legrand P, Huet Y, Larabi S, Rapin M. Diagnosis of central venous catheter-related sepsis: critical level of quantitative tip cultures. Arch Intern Med 1987; 147 : 873-877. |
| [21] | Seddiki SML, Boucherit-OtmaniZ, Boucherit K, Badsi-Amir S, Taleb M, Kunkel D. Assessment of the types of catheter infectivity caused by Candida species and their biofilm formation. First study in an intensive care unit in Algeria. Intrnational Journal of General Medecine 2013; 6: 1-7. |
| [22] | Mermel LA, Allon M, Bouza E, et al. Clinical Practice Guidelines for the Diagnosis and Management of Intravascular Catheter-Related Infection: 2009 Update by the Infectious Diseases Society of America. Clin Infect Dis 2009; 49 : 1-45. |
| [23] | Gürcüoglu E, Akalın H, Ener B, et al. Nosocomial candidemia in adults: Risk and prognostic factors. J Mycol Med 2010; 20 : 269-278. |
| [24] | Mermel LA, Farr BM, Sherertz RJ, et al. Guidelines for the management of intravascular catheter related infections. Clin Infect Dis 2001; 32 : 1249-1272. |
| [25] | World Health Organization: http://www.who.int/gpsc/background/fr/ (06-09-2014 / 16:02 PM). |
| [26] | Eggimann P, Sax H, Pittet D. Catheter-related infections, Microbes Infect 2004; 6: 1033–1042. |
| [27] | Seabra R, Bhogal N. Hospital infections, animal models and alternatives. Eur J Clin Microbiol Infect Dis 2009; 28: 561–568. |
| [28] | Chow JK, Golan Y, Ruthazer R, et al. Factors Associated with Candidemia Caused by Non-albicans Candida Species Versus Candida albicans in the Intensive Care Unit. Clin Infect Dis 2008; 46 : 1206-1213. |
| [29] | Timsit JF, Dubois Y, Minet C, et al. New materials and devices for pre¬venting catheter-related infections. Ann Intensive Care 2011; 1: 34. |
| [30] | Fridkin SK, Jarvis WR. Epidemiology of nosocomial fungal infections. Clin Microbiol Rev 1996; 9: 499-511. |
| [31] | Vincent JL. Nosocomial infections in adult intensive-care units. Lancet 2003; 361: 2068–2077. |
| [32] | Pfaller MA, Diekema DJ. Epidemiology of Invasive Candidiasis: a Persistent Public Health Problem. Clinical Microbiology Reviews 2007; 20 5: 133–163. |
| [33] | Al Mohajer M, Darouiche RO. Prevention and Treatment of Urinary Catheter-Associated Infections. Curr Infect Dis Rep 2013; 15: 116-123. |
| [34] | Donlan RM, Costerton JW. Biofilms: survival mechanisms of clinically relevant microorganisms. Clinical Microbiology Reviews 2002; 15: 167 – 13. |
| [35] | Shoham S. Invasive Candidiasis in Patients with Implants. Curr Fungal Infect Rep 2011; 5: 12-17. |
| [36] | Martinez LR, Fries BC. Fungal Biofilms: Relevance in the Setting of Human Disease. Curr Fungal Infect Rep 2010; 4: 266–75. |
| [37] | Bonhomme J, d’Enfert C. Candida albicans biofilms: building a heterogeneous, drug-tolerant environment. Curr Op Microbiol 2013; 16: 398-403. |
| [38] | Paulitsch AH, Willinger B, Zsalatz B, Stabentheiner E, Marth E, Buzina W. In-vivo Candida biofilms in scanning electron microscopy. Med Mycol 2009 ; 47 : 690-696. |
| [39] | Chandra J, Mukherjee PK, Ghannoum MA. In vitro growth and analysis of Candida biofilms. Nat Protoc 2008; 3: 1909-1924. |
| [40] | Harrison JJ, Ceri H, Yerly J, et al. The use of microscopy and three-dimensional visualization to evaluate the structure of microbial biofilms cultivated in the Calgary Biofilm Device. Biol Proced Online 2006; 8: 194-215. |
| [41] | Bachmann SP, VandeWalle K, Ramage G, et al. In vitro activity of caspofungin against Candida albicans biofilms. Antimicrob Agents Chemother 2002; 46 : 3591-3596. |
| [42] | Mukherjee PK, Zhou G, Munyon R, Ghannoum MA. Candida biofilm: a well-designed protected environment. Med Mycol 2005; 43 : 191-208. |
| [43] | Baillie GS, Douglas LJ. Iron-limited biofilms of Candida albicans and their susceptibility to amphotericin B. Antimicrob Agents Chemother 1998; 42 : 2146 –2149. |
| [44] | Alem MA, Oteef MD, Flowers TH, Douglas LJ. Production of tyrosol by Candida albicans biofilms and its role in quorum sensing and biofilm development. Eukaryot Cell 2006; 5: 1770 - 79. |
| [45] | Nett J, Lincoln L, Marchillo K, Andes D. ß-1,3 Glucan as a Test for Central Venous Catheter Biofilm Infection. The Journal of Infectious Diseases 2007; 195 : 1705 - 1712. |
