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Vol.4 , No. 6, Publication Date: Nov. 14, 2017, Page: 68-71
</i> Twigs Extract on Bacteria Isolated from Human Mouth&description=The phytochemical properties and the antibacterial activity of aqueous extract of neem twigs (<i>Azadirachta indica</i>) on the bacterial species isolated from human mouth was investigated. The neem tree was observed to be devoid of any infection prior to cutting the twigs for laboratory analysis. The mouths of fifteen (15) students were swabbed using a sterile swab stick in the morning before each of them washes their mouth at Federal University Wukari. Bacterial isolation was done using nutrient agar. Identification of the bacteria isolates were done using standard techniques. Agar-well diffusion method was used to determine the antibacterial effect of the extract on a prepared nutrient agar. The twigs extracts showed antibacterial activity against all the test isolates at different concentrations. The zone of inhibition increased with increased concentrations of the extracts. Phytochemical extraction was done using GC-MS QP2010 PLUS machine. Polyphenols were extracted at a very large amount; Saponnins and Flavonoides were found to be in a large quantity while Glycosides and Alkaloids were found to be low. However, Phylobatanins, Anthraguinones, Hydroxymethyl Anthraguinones and Tannins were found to be absent. Most of these compounds extracted from neem twigs have antibacterial activity. Therefore, these results, further confirm the belief and other scientific studies on the importance of the traditional use of neem twigs in maintaining oral hygiene. Again, some of the isolates from the human mouth are potential pathogens and may pose a public health hazard to the community. Therefore, routine oral hygiene are strongly advocated to prevent oral diseases.&picture=http://www.aascit.org/aascit/decorators/img/journal/908.jpg)
| [1] | Ibrahim Awache, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University, Wukari, Nigeria. |
| [2] | Gero Mohammed, Department of Consultancy and Production, National Research Institute for Chemical Technology, Zaria, Nigeria. |
| [3] | Yakubu Michael Nosano, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University, Wukari, Nigeria. |
| [4] | Ubandoma Andefiki, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University, Wukari, Nigeria. |
| [5] | Yusuf Abdullahi, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University, Wukari, Nigeria. |
| [6] | Acha Maryfavour, Department of Microbiology, Faculty of Pure and Applied Sciences, Federal University, Wukari, Nigeria. |
The phytochemical properties and the antibacterial activity of aqueous extract of neem twigs (Azadirachta indica) on the bacterial species isolated from human mouth was investigated. The neem tree was observed to be devoid of any infection prior to cutting the twigs for laboratory analysis. The mouths of fifteen (15) students were swabbed using a sterile swab stick in the morning before each of them washes their mouth at Federal University Wukari. Bacterial isolation was done using nutrient agar. Identification of the bacteria isolates were done using standard techniques. Agar-well diffusion method was used to determine the antibacterial effect of the extract on a prepared nutrient agar. The twigs extracts showed antibacterial activity against all the test isolates at different concentrations. The zone of inhibition increased with increased concentrations of the extracts. Phytochemical extraction was done using GC-MS QP2010 PLUS machine. Polyphenols were extracted at a very large amount; Saponnins and Flavonoides were found to be in a large quantity while Glycosides and Alkaloids were found to be low. However, Phylobatanins, Anthraguinones, Hydroxymethyl Anthraguinones and Tannins were found to be absent. Most of these compounds extracted from neem twigs have antibacterial activity. Therefore, these results, further confirm the belief and other scientific studies on the importance of the traditional use of neem twigs in maintaining oral hygiene. Again, some of the isolates from the human mouth are potential pathogens and may pose a public health hazard to the community. Therefore, routine oral hygiene are strongly advocated to prevent oral diseases.
Keywords
Neem Extracts, Oral Hygiene, Antibacterial Activity, Agar-well Diffusion Method Phytochemical Analysis
Reference
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