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American Journal of Food Science and Nutrition

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Pomegranate Peels Extract Improves Plasma Lipid Profiles in Triton-WR-1339-Induced Hyperlipidemic Mice and Attenuates Lipoprotein Oxidation

American Journal of Food Science and Nutrition
Vol.5 , No. 3, Publication Date: May 30, 2018, Page: 58-65

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Authors

[1]	Mohamed Harnafi, Laboratory of Biochemistry and Biotechnologies, University Mohamed I, Oujda, Morocco.
[2]	Ilham Touiss, Laboratory of Biochemistry and Biotechnologies, University Mohamed I, Oujda, Morocco.
[3]	Saloua Khatib, Laboratory of Biochemistry and Biotechnologies, University Mohamed I, Oujda, Morocco.
[4]	Oussama Bekkouch, Laboratory of Biochemistry and Biotechnologies, University Mohamed I, Oujda, Morocco.
[5]	Hicham Harnafi, Laboratory of Biochemistry and Biotechnologies, University Mohamed I, Oujda, Morocco.
[6]	Souliman Amrani, Laboratory of Biochemistry and Biotechnologies, University Mohamed I, Oujda, Morocco.

Abstract

This study was undertaken to investigate the hypolipidemic and anti-lipoprotein oxidation activities of an aqueous extract from pomegranate peels using Triton WR-1339-induced hyperlipemic mice as experimental model. Hyperlipidemia was developed by intraperitoneal injection of Triton at a dose of 200 mg/kg body weight. The animals were divided into three groups of eight mice each: normolipidemic control group (NCG), hyperlipidemic control group (HCG) and pomegranate peels extract treated group (PTG). After 10 h treatment, Triton caused a significant increase in plasma lipid parameters (total cholesterol, triglycerides, LDL-cholesterol, Atherogenic index and LDL/HDL ratio). However, the administration of pomegranate peels extract significantly reduced the elevated plasma lipid profile induced by triton injection. Although no significant change of HDL-cholesterol levels was noticed after treatment. Furthermore, the aqueous pomegranate peels extract showed a significant ameliorative action on elevated atherogenic index (AI) and LDL/HDL-C ratios. On the other hand, the extract showed effective antiradical activity (CI₅₀=3.67±0.06 µg/ml) against DPPH and significantly protects lipoproteins obtained from hyperlipidemic mice against copper induced oxidation in vitro (P<0.001). The extract contains 394.21±4.55 mg/g total phenolics, 309.27±4.13 mg/g tannins and 67.33±1.26 mg/g flavonoids. This finding indicates that pomegranate peels may contain polar products able to lower plasma lipid concentrations and might be beneficial in treatment of hyperlipidemia and atherosclerosis prevention.

Keywords

Hypocholesterolaemia, Hypotriglyceridaemia, Atherogenic Index, Anti-lipoprotein Oxidation, Pomegranate Peels, Triton WR-1339, Mice

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