Indexing
All Issues
Submission
Author Guidelines
Reviewers
Editorial Members
Publication Fee
Vol.1 , No. 5, Publication Date: Nov. 22, 2014, Page: 76-82
, shrimps (<i>Penaeus notialis</i>), and blue crab (<i>Callinectes sapidus</i>), were collected from landing sites along the Lagos Lagoon, Lagos State Nigeria. Physicochemical parameters of water samples were also monitored using standard methods. Samples were collected for 2 years between 2011- 2013. They were examined for incidence of <i>Vibrio</i> species using both biochemical and molecular methods. Biochemical identification of the isolates was confirmed with Polymerase Chain Reaction (PCR) and nucleotide sequencing using V. 16S-700F and V. 16S- 325R primers. 27 isolates were obtained from the samples examined. Dissolved oxygen and conductivity showed a significant difference (P < 0.05) in the Lagos lagoon. 16 isolates were presumptively identified as <i>Vibrio</i> species. PCR identified 7 isolates as <i>Vibrio</i> species; DNA sequencing identified the 7 isolates as <i>V. parahaemolyticus</i> (5), <i>V. fluvialis</i> (1) and <i>Shewanella algae</i> (1). The results reveals incidence of Vibrio species in seafood samples from Lagos lagoon.&picture=http://www.aascit.org/aascit/decorators/img/journal/907.jpg)
| [1] | Chigozie Oramadike, Fish Technology Department, Nigerian Institute for Oceanography and marine Research, P. M. B. 12729, Victoria Island Lagos, Nigeria. |
| [2] | Samuel Temitope Ogunbanwo, Department of Microbiology, University of Ibadan, Ibadan, Nigeria. |
Vibrio species are natural inhabitant of the marine and estuarine environment which often causes food-borne gastroenteritis. A total of 90 seafood samples, croaker fish (Pseudotolithus senegalensis), shrimps (Penaeus notialis), and blue crab (Callinectes sapidus), were collected from landing sites along the Lagos Lagoon, Lagos State Nigeria. Physicochemical parameters of water samples were also monitored using standard methods. Samples were collected for 2 years between 2011- 2013. They were examined for incidence of Vibrio species using both biochemical and molecular methods. Biochemical identification of the isolates was confirmed with Polymerase Chain Reaction (PCR) and nucleotide sequencing using V. 16S-700F and V. 16S- 325R primers. 27 isolates were obtained from the samples examined. Dissolved oxygen and conductivity showed a significant difference (P < 0.05) in the Lagos lagoon. 16 isolates were presumptively identified as Vibrio species. PCR identified 7 isolates as Vibrio species; DNA sequencing identified the 7 isolates as V. parahaemolyticus (5), V. fluvialis (1) and Shewanella algae (1). The results reveals incidence of Vibrio species in seafood samples from Lagos lagoon.
Keywords
Seafoods, Vibrio Species, Sequencing, Primers
Reference
| [01] | Austin, B. 2010. Vibrios as causal agents of zoonoses. Veterinary Microbiology 140: 310-317. |
| [02] | Igbinosa, E.O. 2010. Surveillance of invasive Vibrio species in discharged aqueous effluents of wastewater treatment plants in the Eastern Cape province of South Africa. PhD Thesis, University of Fort Hare Alice, South Africa. xiv + 109 |
| [03] | Adagbada, A.O, Adesida, S.A., Nwokorie, F.O., Niemogha, M. and Coker, A.O. 2012. Cholera Epidemiology in Nigeria an overview. the pan African medical journal 12: 59. |
| [04] | Farmer III, J. J., Janda, J. M. and BirkHead, K. 2003. Vibrio. In: Murray, P. R., Baron, E. J., Jorgensen, J. A., Pfaller, M. A. & Yolken, R. H. (ed). Manual of Clinical Microbiology (8edn) Vol 1. Washingtoh DC: ASM Press. 706-719. |
| [05] | McLaughlin, J.B., DePaola, A., Bopp, C.A. , Martinek, K.A., Napolilli, N.P., Allison, C.G., Murray, S.L., Thompson, E.C., Bird, M.M. and Middaugh, J.P.. 2005. Outbreak of Vibrio parahaemolyticus gastroenteritis associated with Alaskan oysters. The New England Journal of Medicine 353: 1463-1470. |
| [06] | Utsalo, S.J, Mboto, C.I, Gemade, E.I.I, Nwangwa, M.A.1988.Halophilic Vibrio spp. associated with hard clams (Mercenaria spp.) from the Calabar river estuary. Trans R Soc Trop Med Hyg;82: 327-9. |
| [07] | Eja, M., Abriba, C., Etok, C., Ikpeme, E., Arikpo, G., Enyi-Idoh, K. & Ofor, U. 2008. Seasonal occurrence of Vibrios in water and shellfish obtained from Great Kwa River Estuary, Calabar, Nigeria. Bull Environ Cont Toxicol 81. 245-248. |
| [08] | Lutz, C., Erken, M., Noorian, P., Sun, S. and McDougald, D. 2013. Environmental reservoirs and mechanisms of persistence of Vibrio cholerae. Front Microbiol. 4: 375-380. |
| [09] | Hassan, Z.H., Zwartkruis-Nahuis, J.T.M. and de Boer, E.2012. Occurrence of Vibrio parahaemolyticus in retailed seafood in the Netherlands. International Food Research Journal 19.1: 39-43. |
| [10] | Martinez-Urtaza, J., Lozano-Leon, A., Varela-Pet, J., Trinanes, J., Pazos, Y., Garcia-Martin, O., 2008. Environmental determinants of the occurrence and distribution of Vibrio parahaemolyticus in the rias of Galicia, Spain. Applied and Environmental Microbiology 74: 265–274. |
| [11] | Thompson, F.L., Lida, T. and Swings, J. 2004 Biodiversity of vibrios. Microbiology and Molecular Biology Reviews 68: 403-431. |
| [12] | Rademaker, D. L.W., Hoste, B., Louws, F. J., Kerster, K., Swings, J., Vauterin, L., Vauterin, P. & Bruijin, F. 2000. Comparison of AFLP and rep- PCR genomic fingerprinting with DND-DNA homology studies: Xanthomonas as a mode system. Int J. syst Evol microbiol 68.3: 403. |
| [13] | Gurtler, V. and Mayall, B. 2001. Genomic approaches to typing, taxonomy of bacterial isolates. Int J Syst Evol Microbiol 5: 3-16. |
| [14] | Thompson, F.L. and Swings, J. 2006. Taxonomy of the Vibrios. In : Thompson, F.L., Austin, B., Swings, J. (Eds.), The Biology of Vibrios. ASM press Washington D.C. 29-43. |
| [15] | Thompson, C. C., Vicente, A. C., Souza, R. C., Vasconcelos, A. T., Vesth, T., Alves, N. Jr. 2009. Genomic taxonomy of Vibrios. BMC Evol Biol 9:258. |
| [16] | Chan, J. Z.-M., Halachev, M. R., Loman, N. J., Constantinidou, C., and Pallen, M. J. 2012. Defining bacterial species in the genomic era: insights from the genus Acinetobacter. BMC Microbiol 12:302. |
| [17] | Ajao, E.A. 1990. The influence of domestic and industrial effluents on population of sessile and benthic organisms in Lagos Lagoon. Ph.D thesis. University of Ibadan, Nigeria. 412-413. |
| [18] | Nkwoji, J.A., Yakub, A., Ajani, G.E., Balogun, K.J., Igbo, J.K, Renner , K. O., Ariyo, A. A. and Bello B. O. 2010. Seasonal variations in the water chemistry and benthic macroinvertebrates of a South Western Lagoon, Lagos, Nigeria. J Am Sci 6: 85-92. |
| [19] | Elliot, E.L., Kaysner, C.A., Jackson, L. and Tamplin, M.L. 1998. Vibrio cholerae, V. parahaemolyticus, V. vulnificus and other Vibrio species. Ch.9. In: Food and Drug Administration Bacteriological Manual, 8th ed. (Revision A), (CR-ROM version). R. L. Merker (ed.). AOAC International, Gaitheisbarg, MD. Pp15-48. |
| [20] | Adeleye, A., Eyinnia, V., Nwanze, R., Smith, S. and Omonigbehin, E. 2008. Antimicrobial susceptibility of potentially pathogenic halophilic Vibrio isolated from seafoods in Lagos, Nigeria. Afr. J. Biotechnol 7: 3791-3794. |
| [21] | Troisa, C., Coulonb F., Combreta, C. P., Martinsc, J. M. F., Oxarangoc, L. 2010. Effect of pine bark and compost on the biological dentrification process of non hazardous landfill leachate: focus on the microbiology. Journal of Hazardous materials 181: 1163-1169. |
| [22] | Amin, R. A. and Salem, A. M. 2012. Specific Detection of pathogenic Vibrio species in shellfish by using multiplex polymerase chain reaction. Global Veterinaria Idosi publications 8.5: 525-531. |
| [23] | Espeñeira, M., Atanassova, M., Vieites, J.M. and Santaclara, F.J. 2010. Validation of a method for the detection of five species, serogroup, biotypes and virulence factors by multiplex PCR in fish and seafood. J Food Microbiol 27. 122-131. |
| [24] | Hammer, O. Harper, D. A. T. and Rayan P. D. 2001. PAST version 1.49 (palaeontological statistics software package for education and data analysis), statistical software. Palaeontologia Electronica 4 .1: 9. |
| [25] | Eyisi, O.A., Nwodo, U.U. and Iroegbu, C.U. 2013. Distribution of Vibrio species in shellfish and water samples collected from the Atlantic coastline of south-east Nigeria. J Health Popul Nutr 31. 3: 314-20. |
| [26] | Tantillo, G.M., Fontanarosa, M., Di Pinto, A. and Musti, M., 2004. A Review Updated perspectives on emerging vibrios associated with human infections. Letters in Appl Microbiol 39:117–126. |
| [27] | Frans, I., Michiels, C.W., Bossier, P., Willems, K. A., Lievens, B., Rediers, H. 2011. Vibrio anguillarum as a fish pathogen: virulence factors, diagnosis and prevention. J Fish Dis 34, 9: 643-661. |
| [28] | Adejuwon, J.O. and Adelakun, M. A. 2013. Physiochemical and bacteriological analysis of surface water in Ewekoro local government area of Ogun State, Nigeria: Case study of Lala, Yobo and Agodo rivers. Int J Water Resources Environ Eng 4: 66-72. |
| [29] | Ideriah, T.J.K., O. Amachree and H.O. Stanley, 2010. Assessment of water quality along amadi creek in port harcourt, Nigeria. Sci. Afr 9: 150-162. |
| [30] | Adeleye, I. A., Daniels F. V. and Enyinnia V. A. 2010. Characterization and pathogenicity of Vibrio spp. contaminating seafoods in Lagos, Nigeria. Internet J. food safety 12: 1-9. |
| [31] | Holt, J. G., and Krieg, N. R. 1994. Bergey's manual of determinative microbiology, 9th ed. p. 190-274. The Williams & Wilkins Co., Baltimore, Md. |
| [32] | Gopal, S., Otta, S.K., Kumar, S. 2005. The occurrence of Vibrio species in tropical shrimp culture environments; implication for food safety. Int J Food Microbiol 102:151–159. |
| [33] | Hosseini, H., Cheraghali, A.M., Yalfani, R. and Razavilar, V. 2004. Incidence of Vibrio spp. in shrimp caught off the south coast of Iran. Food Control 15: 187-190. |
| [34] | Nwachukwu, 2006. Studies on the pathogenic characteristics of non 01 Vibrio. Cholera isolated from seafoods in Nigeria. J Eng Appl sci 1: 284-287. |
| [35] | Adebayo-Tayo, B. C., Okonko, I. O., John, M.O., Odu, N. N., Nwanze, J. C. and Ezediokpu, M. N. 2011. Occurrence of potentially pathogenic Vibrio species in seafoods obtained from Oron creek. Advances in biological research Idosi publications 5.6: 356-365. |
| [36] | Nsofor, C.A., Kemajou, S. T., Nsofor,C. M., 2014. Incidence and antibiotic susceptibility pattern of Vibrio species isolated from seafoods sold in Portharcourt Nigeria. Academic journals 6. 2: 13-16. |
| [37] | Sathiyamurthy,K., Baskaran, A and Kumar, S. D. 2013. Prevalence of Vibrio cholerae and Other Vibrios from Environmental and Seafood Sources,Tamil Nadu, India british microbiology research journal 3. 4: 538-549. |
| [38] | Hasan, F., Kamuzaman, M., Meklones, J., Faruque, S. M. Satelite phage 2010. TLC pro phage enables toxigenic conversion by CTX phage through dif site alteration. Nature. 467:982-985. |
| [39] | Balckston, G.M., Nordstrom, J.L., Bowen, M..D.2007. Use of the real time PCR asay for the detection of the ctxA gene of Vibrio cholerae in an environmental survey of Mobile Bay. J Microbiol Meth 68:254-259. |
| [40] | Ottaviani, D., Santarelli, S., Bacchiocchi, S. 2005. Presence of pathogenic Vibrio parahaemolyticus strains in mussels from the Adriatic Sea, Italy. Food Microbiol 22:585–590. |
| [41] | Mahmud, Z., Kassu, A., Mohammad, A., 2006. Isolation and characterization of toxigenic Vibrio parahaemolyticus from the Kii Channel, Japan. Microbiol Res 161:25–37. |
| [42] | Di Pinto, A., Ciccarese, G., De Corato, R. 2008. Detection of pathogenic Vibrio parahaemolyticus in southern Italian Shellfish. Food Control 19:1037–1041. |
