ISSN: 2375-3765
American Journal of Chemistry and Application  
Manuscript Information
 
 
Chemometry of Clinically Importent Fatty Acids in the Blood Serum Using Near Infrared Spectrometer
American Journal of Chemistry and Application
Vol.5 , No. 3, Publication Date: May 30, 2018, Page: 45-50
455 Views Since May 30, 2018, 323 Downloads Since May 30, 2018
 
 
Authors
 
[1]    

Andrei Kalinin, Institute of Spectroscopy, Russian Academy of Sciences, Moscow, Troitsk, Russia.

[2]    

Victor Krasheninnikov, Institute of Spectroscopy, Russian Academy of Sciences, Moscow, Troitsk, Russia.

[3]    

Alexandr Sviridov, Institute on Photon Technologies of Federal Scientific Research Centre «Crystallography and Photonics», Russian Academy of Sciences Moscow, Troitsk, Russia.

[4]    

Vladimir Titov, Russian Cardiology Research and Production Complex, Ministry of Health, Moscow, Russia.

 
Abstract
 

Triglycerides of fatty acids (FAs) are essential components of the human diet, while possessing an unique biological activity. The correlation of some diseases with the ratios of triglycerides of the saturated, monounsaturated and polynoic FAs in blood of patients is widely known. The content of FAs in biological media is traditionally determined using gas or liquid chromatographic methods. These methods are inaccessible to clinical laboratories because of their inefficiency, complexity and high cost. Thus, our goal was to develop a method and technical conditions for rapid assay in the serum of these FAs, namely, saturated palmitic, monounsaturated oleic and double-unsaturated linoleic, and polynoic arachidonic FAs with the use of a near infrared spectrometer and a regression (calibration) method on the projections of latent structures. As a result, the correlation between the absorption spectra of the serum samples with the mass fractions of named FAs and total triglycerides and cholesterol was established. Parameters of the calibration models and the conditions of spectrometry were assessed. Limit sensitivity 0.1 mg/l of arachidonic FA was achieved. So, the applicability of the FT spectrometer in the wavelength range 1.0-1.8 μ for the analysis of the named FAs in serum without sample pretreatment was confirmed.


Keywords
 

Fatty Acids, Blood Serum, Near Infrared FT Spectrometer


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