Indexing
All Issues
Submission
Author Guidelines
Reviewers
Editorial Members
Publication Fee
Vol.5 , No. 3, Publication Date: May 30, 2018, Page: 45-50
 are essential components of the human diet, while possessing an unique biological activity. The correlation of some diseases with the ratios of triglycerides of the saturated, monounsaturated and polynoic FAs in blood of patients is widely known. The content of FAs in biological media is traditionally determined using gas or liquid chromatographic methods. These methods are inaccessible to clinical laboratories because of their inefficiency, complexity and high cost. Thus, our goal was to develop a method and technical conditions for rapid assay in the serum of these FAs, namely, saturated palmitic, monounsaturated oleic and double-unsaturated linoleic, and polynoic arachidonic FAs with the use of a near infrared spectrometer and a regression (calibration) method on the projections of latent structures. As a result, the correlation between the absorption spectra of the serum samples with the mass fractions of named FAs and total triglycerides and cholesterol was established. Parameters of the calibration models and the conditions of spectrometry were assessed. Limit sensitivity 0.1 mg/l of arachidonic FA was achieved. So, the applicability of the FT spectrometer in the wavelength range 1.0-1.8 μ for the analysis of the named FAs in serum without sample pretreatment was confirmed.&picture=http://www.aascit.org/aascit/decorators/img/journal/905.jpg)
| [1] | Andrei Kalinin, Institute of Spectroscopy, Russian Academy of Sciences, Moscow, Troitsk, Russia. |
| [2] | Victor Krasheninnikov, Institute of Spectroscopy, Russian Academy of Sciences, Moscow, Troitsk, Russia. |
| [3] | Alexandr Sviridov, Institute on Photon Technologies of Federal Scientific Research Centre «Crystallography and Photonics», Russian Academy of Sciences Moscow, Troitsk, Russia. |
| [4] | Vladimir Titov, Russian Cardiology Research and Production Complex, Ministry of Health, Moscow, Russia. |
Triglycerides of fatty acids (FAs) are essential components of the human diet, while possessing an unique biological activity. The correlation of some diseases with the ratios of triglycerides of the saturated, monounsaturated and polynoic FAs in blood of patients is widely known. The content of FAs in biological media is traditionally determined using gas or liquid chromatographic methods. These methods are inaccessible to clinical laboratories because of their inefficiency, complexity and high cost. Thus, our goal was to develop a method and technical conditions for rapid assay in the serum of these FAs, namely, saturated palmitic, monounsaturated oleic and double-unsaturated linoleic, and polynoic arachidonic FAs with the use of a near infrared spectrometer and a regression (calibration) method on the projections of latent structures. As a result, the correlation between the absorption spectra of the serum samples with the mass fractions of named FAs and total triglycerides and cholesterol was established. Parameters of the calibration models and the conditions of spectrometry were assessed. Limit sensitivity 0.1 mg/l of arachidonic FA was achieved. So, the applicability of the FT spectrometer in the wavelength range 1.0-1.8 μ for the analysis of the named FAs in serum without sample pretreatment was confirmed.
Keywords
Fatty Acids, Blood Serum, Near Infrared FT Spectrometer
Reference
| [01] | VN Titov, AV Aripovsky, SI Kaba, PO Kolesnik, MI Vezdel, Yu. K. Shiryaeva, Clinical laboratory diagnostics, No. 7 (2012) 3- 8 (in Russian) |
| [02] | University of Maryland Medical Center, available at: http://umm.edu/health/medical/altmed/supplement/omega3-fatty-acids accessed 26.10.2016. |
| [03] | D. J. Holub and B. J. Holub, Omega-3 fatty acids from fish oils and cardiovascular disease, Molecular and Cellular Biochemistry, 2004, 263, 217–225. |
| [04] | A. Kh. Kadyrov, F. Kh. Mansurova, MB Toshev, M. M. Suriev, Determination of the content of higher fatty acids in the sera of healthy individuals and patients with cholelithiasis at various stages of lithogenesis, Reports of the Academy of Sciences of the Republic Tajikistan, 2008, 51, No. 11, 858-863,. (in Russian). |
| [05] | Yang Y. J., Choi M. H., Paik M. J., Yoon H. R., Chunga B. C., Gas chromatographic–mass spectrometric determination of plasma saturated fatty acids using pentafluorophenyldimethylsilyl derivatization. J Chromatogr. B, 2000, 742, pp. 37-46. |
| [06] | Yi L. Z., He J., Liang Y. Z., Yuan D. L., Chau F.-T. Plasma fatty acid metabolic profiling and biomarkers of type 2 diabetes mellitus based on GC/MS and PLS-LDA. FEBS Letters, 2006, 580, pp. 6837-6845. |
| [07] | E. Á. Hernández, et all., Acute dietary fat intake initiates alterations in energy metabolism and insulin resistance, The Journal of Clinical Investigation, 2017, 127, №2, 695-708. |
| [08] | L. C. Hudgins. J. Hirsch and E. A. Emken, Correlation of isomeric fatty acids in human adipose tissue with clinical risk factors for cardiovascular diseas, Am J Clin Nutr, 1991: 53: 474-82. |
| [09] | R. J. de Souza, et all, Intake of saturated and trans unsaturated fatty acids and risk of all cause mortality, cardiovascular disease, and type 2 diabetes: systematic review and meta-analysis of observational studies, BMJ 2015; 351: h3978 | doi: 10.113 6/bmj.h3978 |
| [10] | C. Petibois, G. Cazorla, A. Cassaigne, and G. Déléris, Application of FT-IR Spectrometry to Determine the Global Metabolic Adaptations to Physical Conditioning in Sportsmen, Applied Spectroscopy (2002), 56, № 10, pp. 1259-1267. |
| [11] | Wold S., Sjostrom M., Eriksson L., PLS regression, Chem. Intelligent. Lab. Syst. 58 (2001) 7: 109–30. |
| [12] | Kalinin A., Krasheninnikov V., Sadovskiy S., Denisovich E., Yurova E., J. Near Infrared Spectr., 16 (2008) 3: 343–8. |
| [13] | A. V. Kalinin, V. N. Krasheninnikov, A. P. Sviridov, V. N. Titov, Journal of Applied Spectroscopy, 2016, 83, N5, pp. 811-819, |
| [14] | A. V. Kalinin, V. N. Krasheninnikov, A. P. Sviridov, V. N. Titov, Clinical laboratory diagnostics, 60 (2015) №11 13-20 (in Russian) |
| [15] | F. Westad, A. Schmidt and M. Kermit, J. Near Infrared Spectrosc. 16 (2008) 3: 265-73. |
| [16] | Titov V. N, Pathogenesis. 2013; 11 (1): 18–26. |
