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Vol.4 , No. 6, Publication Date: Dec. 5, 2017, Page: 55-58
 is cultivated for economic and medicinal purposes. <i>G. barbadense</i> fruit volatiles were evaluated for the first time in order to ascertain the cytotoxic efficacy on cancerous cells. The essential oil was prepared by hydro-distillation and characterized by gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of <i>G. barbadense</i> fruit oil on human cervix adenocarcinoma (HeLa) and mouse fibroblast (3T3) cell lines were determined using the MTT-based cytotoxicity assay. Thirteen constituents were identified accounting for about 100% of the total oil composition. The oil was characterized by a significant amount of β-caryophyllene (40.97%), α-pinene (15.9%), α-caryophyllene (12.15%), and α-copaene (8.48%). This oil also exhibited notable cytotoxic activity against HeLa and 3T3 cell lines with the IC<sub>50</sub> values of 47.0 µg/mL and 59.5 µg/mL respectively. <i>G. barbadense</i> fruit oil is a promising candidate for evaluation of various cell lines as a result of its rich β-caryophyllene content and peculiar compositional profile.&picture=http://www.aascit.org/aascit/decorators/img/journal/905.jpg)
| [1] | Emmanuel Emmanuel Essien, Department of Chemistry, University of Uyo, Uyo, Nigeria; Department of Chemistry, University of Ibadan, Ibadan, Nigeria. |
| [2] | Ekpeno Josiah Nkop, Department of Chemistry, University of Ibadan, Ibadan, Nigeria; Hussain Ebrahim Jamal Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan. |
| [3] | Mohammad Iqbal Choudhary, Hussain Ebrahim Jamal Research Institute of Chemistry, International Centre for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan. |
Gossypium barbadense (cotton plant) is cultivated for economic and medicinal purposes. G. barbadense fruit volatiles were evaluated for the first time in order to ascertain the cytotoxic efficacy on cancerous cells. The essential oil was prepared by hydro-distillation and characterized by gas chromatography-mass spectrometry (GC-MS). The cytotoxic effects of G. barbadense fruit oil on human cervix adenocarcinoma (HeLa) and mouse fibroblast (3T3) cell lines were determined using the MTT-based cytotoxicity assay. Thirteen constituents were identified accounting for about 100% of the total oil composition. The oil was characterized by a significant amount of β-caryophyllene (40.97%), α-pinene (15.9%), α-caryophyllene (12.15%), and α-copaene (8.48%). This oil also exhibited notable cytotoxic activity against HeLa and 3T3 cell lines with the IC50 values of 47.0 µg/mL and 59.5 µg/mL respectively. G. barbadense fruit oil is a promising candidate for evaluation of various cell lines as a result of its rich β-caryophyllene content and peculiar compositional profile.
Keywords
Gossypium barbadense, Malvaceae, Essential Oil, Cytotoxicity
Reference
| [01] | Gotmare, V., Singh, P., Tule, B. Wild and Cultivated Species of Cotton, Vol. 5. In: Technical Bulletin; Central Institute for Cotton Research: Nagpur, India; 2000. |
| [02] | Busari, A. A. An annotated booklet of some medicinal plants ofSouth-west Nigeria. Armetu Publisher, Ibadan; 1984, p. 70. |
| [03] | Opitz, S., Kunert, G., Gershenzon, J. Increased terpenoid accumulation in cotton (Gossypium hirsutum) foliageis a general wound response. J. Chem. Ecol. 2008, 34: 508–522. |
| [04] | Degenhardt, J., Köllner, T. G., Gershenzon, J. Monoterpene and sesquiterpene synthases and the origin ofterpene skeletal diversity in plants. Phytochem. 2009, 70: 1621–1637. |
| [05] | Pare, P. W., Tumlinson, J. H. De novo biosynthesis of volatiles induced by insect herbivory in cotton plants. Plant Physiol. 1997, 114: 1161–1167. |
| [06] | Rose, U. S. R., Tumlinson, J. H. Systemic induction of volatile release in cotton: How specific is the signalto herbivory? Planta Medica 2005, 222: 327–335. |
| [07] | Loughrin, J. H., Manukian, A., Heath, R. R., Turlings, T. C., Tumlinson, J. H. Diurnal cycle of emission of inducedvolatile terpenoids by herbivore-injured cotton plant. Proc. Natl. Acad. Sci. USA 1994, 91: 11836–11840. |
| [08] | Hedin, P. A., Thompson, A. C., Gueldner, R. C., Rizk, A. M., Salama, H. S. Egyptian cotton leaf essential oil. Phytochem. 1972, 11: 2356-2357. |
| [09] | Essien, E. E., Aboaba, S. O., Ogunwande, I. A. Constituents and antimicrobial properties of the essential oil of Gossypium barbadense (Linn.) leaf. J. Med. Plants Res. 2011, 5 (5): 702-705. |
| [10] | Perveen, S. S., Qaisrani, T. M., Siddiqui, F., Perveen, R., Naqvi, S. H. M. Cotton plant volatiles and insect’s behavior. Pak. J. Biol. Sci. 2001, 4 (5): 554-558. |
| [11] | Medicines and Healthcare products Regulatory Agency (MHRA). British Pharmacopoeia; H. M. Stationery Office, Pharmaceutical Press: London, UK, 1980. |
| [12] | Adams, R. P. Identification of Essential Oil Components by Gas Chromatography/Mass Spectrometry, 4th ed; Allured Publishing Corp: Carol Stream, IL, USA, 2007. |
| [13] | Mosmann, T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J. Immunol. Meth. 1983, 65: 55–63. |
| [14] | Bell, A. A. Physiology of Secondary Products. In: Cotton Physiology; Mauney, J. R., Stewart, J. M. (Eds). The Cotton Foundation: Memphis, TN, USA; 1986, pp. 597–621. |
| [15] | Langhasova, L., Hanusova, V., Rezek, J., Stohanslova, B., Ambroz, M., Kralova, V., et al. Essential oil from Myrica rubra leaves inhibits cancer cell proliferation and induces apoptosis in several human intestinal lines. Ind. Crops Prod. 2014, 59: 20–26. |
| [16] | Fidyt, K., Fiedorowicz, A., Strządała, L., Szumny, A. β‐caryophyllene and β‐caryophyllene oxide-natural compounds of anticancer and analgesic properties. Cancer Med. 2016, 5 (10): 3007–3017. |
| [17] | Gowda, P. J., Ramakrishnaiah, H., Krishna, V., Jagannath, N. Caryophyllene-rich essential oil of Didymocarpus tomentosa: chemical composition and cytotoxic activity. Nat. Prod. Commun. 2012, 7 (11): 1535-1538. |
