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Vol.3 , No. 2, Publication Date: Mar. 24, 2016, Page: 6-12
 using different extraction solvents. Deionized distilled water (ddH2O), 80 methanol (80 MeOH), 70 ethanol (70 EtOH), and 80 acetone were used as solvents. The antioxidant activities of the OLEs were observed using DPPH, β-carotene bleaching, and antioxidant capacity standard assays. Highest total phenol, flavonoid and vitamin c contents of the OLEs was observed to be 26.15 mg TAE/g dry weight powder (DW), 25.67 mg catechin/g DW and 198.7mg ascorbic acid/g DW, respectively. GC-MS analysis revealed the presence of bioactive compounds such as oleuropein, caffeic acid, vanillin, rutin, glucosides, quercetin, luteolin, apigenin, and traces of chryseriol. The antioxidant activities of OLEs showed variation according to the solvent used. Higher radical scavenging activity (91.2 µg/ml) was observed in 80 MeOH solvent. There is a significant (p<0.05) positive correlation between the total phenolics contents and extract’s antioxidant activities. The data indicated that OLEs is a promising source of potential antioxidants and may serve as alternative therapeutic agent in the pathogenesis of some diseases.&picture=http://www.aascit.org/aascit/decorators/img/journal/905.jpg)
| [1] | Bassem Yousef Sheikh, Scientific Miracles of Prophetic Medicine, Faculty of Medicine, Taibah University, Al-Madinah Al-Munawarah, Saudi Arabia. |
| [2] | Sami Gabr, King Saud University, Riyadh, Saudi Arabia. |
The geographical origin of plant significantly affects its phytochemicals constituents. This study evaluated the antioxidants activities of Saudi Arabian olive leave extracts (OLEs) using different extraction solvents. Deionized distilled water (ddH2O), 80 methanol (80 MeOH), 70 ethanol (70 EtOH), and 80 acetone were used as solvents. The antioxidant activities of the OLEs were observed using DPPH, β-carotene bleaching, and antioxidant capacity standard assays. Highest total phenol, flavonoid and vitamin c contents of the OLEs was observed to be 26.15 mg TAE/g dry weight powder (DW), 25.67 mg catechin/g DW and 198.7mg ascorbic acid/g DW, respectively. GC-MS analysis revealed the presence of bioactive compounds such as oleuropein, caffeic acid, vanillin, rutin, glucosides, quercetin, luteolin, apigenin, and traces of chryseriol. The antioxidant activities of OLEs showed variation according to the solvent used. Higher radical scavenging activity (91.2 µg/ml) was observed in 80 MeOH solvent. There is a significant (p<0.05) positive correlation between the total phenolics contents and extract’s antioxidant activities. The data indicated that OLEs is a promising source of potential antioxidants and may serve as alternative therapeutic agent in the pathogenesis of some diseases.
Keywords
Antioxidants, Alternative Medicine, DPPH, Free Radicals, Olive, Phytochemicals, Phytotherapy
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