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Vol.2 , No. 4, Publication Date: Aug. 5, 2015, Page: 61-65
, are drugs which extracted from a tree planted in Thailand, Myanmar and other Southeast Asian countries. Nowadays, kratom compounds are used as recreational drugs in many countries all over the world. The previous report studied a novel method for screening and identification of mitragynine and 7-hydroxymitragynine in human urine by liquid chromatography tandem mass spectrometry. However, the method conditions were not optimized. The objective of this study is to explore a rapid approach to separate and identify the kratom compounds by new instrumentation- ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was utilized to perform method development and optimization. Experiments were carried out using a state-of-the-art ultra high performance liquid chromatography system. The method was found linear in the range of 10 - 100 ng/mL. In the linearity study, good regression equation and correlation coefficient were achieved. Comparing to HPLC system, UHPLC exhibited short analysis time and high selectivity. The optimized method can be used for the separation and detection of mitragynine and 7-hydroxymitragynine.&picture=http://www.aascit.org/aascit/decorators/img/journal/905.jpg)
| [1] | Zhanglei Li, Nanning Environment Supervising Detachment, Nanning, Guangxi, China. |
Chromatography coupling to mass spectrometry becomes a vital instrumentation tool for the separation and determination of small molecules qualitatively and quantitatively. In this study, the author is focused on the mitragynine and 7- hydroxymitragynine. Mitragynine and its major metabolite, 7-hydroxymitragynine (also known as “kratom”), are drugs which extracted from a tree planted in Thailand, Myanmar and other Southeast Asian countries. Nowadays, kratom compounds are used as recreational drugs in many countries all over the world. The previous report studied a novel method for screening and identification of mitragynine and 7-hydroxymitragynine in human urine by liquid chromatography tandem mass spectrometry. However, the method conditions were not optimized. The objective of this study is to explore a rapid approach to separate and identify the kratom compounds by new instrumentation- ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Ultra high performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) was utilized to perform method development and optimization. Experiments were carried out using a state-of-the-art ultra high performance liquid chromatography system. The method was found linear in the range of 10 - 100 ng/mL. In the linearity study, good regression equation and correlation coefficient were achieved. Comparing to HPLC system, UHPLC exhibited short analysis time and high selectivity. The optimized method can be used for the separation and detection of mitragynine and 7-hydroxymitragynine.
Keywords
Kratom, Chromatography, Mass Spectrometry, Method Development
Reference
| [01] | Jansen KLR, Prast CJ. Psychoactive properties of mitragynine (kratom). Journal of Psychoactive Drugs 1988;20(4):455-457. |
| [02] | Assanangkornchai S, Muekthong A, Sam-angsri N, Pattanasattayawong U. The use of mitragynine speciosa (“krathom”), an addictive plant, in Thailand. Substance Use & Misuse 2007;42(14):2145-2157. |
| [03] | Fu H, Cid FX, Dworkin N, Cocores J, Shore G. Screening and identification of mitragynine and 7-hydroxymitragynine in human urine by lc-ms/ms. Chromatography 2015;2(2):253-264. |
| [04] | Merola G, Fu H, Tagliaro F, Macchia T, McCord BR. Chiral separation of 12 cathinone analogs by cyclodextrin‐assisted capillary electrophoresis with uv and mass spectrometry detection. Electrophoresis 2014;35(21-22):3231-3241. |
| [05] | Merola G, Fu H, Tagliaro F, Macchia T, McCord B. Chiral separation of 12 cathinone analogs by cyclodextrin-assisted capillary electrophoresis time-of-flight mass spectrometry. In the 41st International Symposium and Exhibit on High Performance Liquid Phase Separations and Related Techniques (HPLC 2014), New Orleans, LA, USA, 2014. |
| [06] | Yang D, Li Z, Diwu YA, Fu H, Liao J, Wei C, Diwu Z. A novel fluorogenic coumarin substrate for monitoring acid phosphatase activity at low ph environment. Current chemical genomics 2008;2:48. |
| [07] | Zhao C, Arroyo-Mora LE, DeCaprio AP, Sharma VK, Dionysiou DD, O'Shea KE. Reductive and oxidative degradation of iopamidol, iodinated x-ray contrast media, by fe(iii)-oxalate under uv and visible light treatment. Water Research 2014;67:144-153. |
| [08] | Zhao C, Pelaez M, Dionysiou DD, Pillai, SC, Byrne JA, O'Shea KE. Uv and visible light activated tio2 photocatalysis of 6-hydroxymethyl uracil, a model compound for the potent cyanotoxin cylindrospermopsin. Catalysis Today 2014;224:70-76. |
| [09] | Liu S, Zhao Y, Ma F, Ma L, O'Shea K, Zhao C, Hu X, Wu M. Control of microcystis aeruginosa growth and associated microcystin cyanotoxin remediation by electron beam irradiation (ebi). RSC Advances 2015;5(40):31292-31297. |
| [10] | Zhao C, Sharma V, Dionysiou D, O'Shea K. In Oxidation of cylindrospermopsin and its model compound 6-hydroxymethyl uracil by ferrate (vi), Abstracts of Papers of the American Chemical Society 2014; American Chemical Society, Washington, DC, USA. |
