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Vol.2 , No. 1, Publication Date: Mar. 3, 2015, Page: 1-10
 on the growth, photosynthetic activity and antioxidant enzymes of Chlorella pyrenoidosa using 96 h growth tests in a batch-culture system. Results showed that the growth of C. pyrenoidosa was significantly inhibited by [C8mim]Br, which would destroy the photosynthetic system II (PSII), and hinder the photosynthetic electron transfer of C. pyrenoidosa. By changing the photosynthetic pigments, C. pyrenoidosa attempted to repair the destruction of PSII system and capture more light quanta for photosynthesis. Moreover, remarkable physiological and biochemical responses, especially for the antioxidant enzymes in C. pyrenoidosa, occurred in [C8mim] Br treatments. [C8mim]Br increased total soluble protein content and enhanced antioxidant enzymes activities at low concentrations, but inhibited them at high concentrations. These observations indicated that moderate [C8mim]Br stress would stimulate the synthesis of proteins against stress and quenching of free radicals. And the general increase of MDA contents suggested that the physiological effects of [C8mim]Br were probably exerted through free radical generation. Thus, we suggest that it is necessary to evaluate influences of ionic liquids before their release into the natural environment in order to predict their impacts, avoiding irreparable damages.&picture=http://www.aascit.org/aascit/decorators/img/journal/904.jpg)
| [1] | Xiang-Yuan Deng, College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China; Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing, China. |
| [2] | Jie Cheng, College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China. |
| [3] | Kun Gao, College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, China. |
| [4] | Chang-Hai Wang, Jiangsu Provincial Key Laboratory of Marine Biology, Nanjing Agricultural University, Nanjing, China. |
In this present study, indoor experiments were performed to analyze and evaluate the effects of 1-octyl-3-methylimidazolium bromide ([C8mim]Br) on the growth, photosynthetic activity and antioxidant enzymes of Chlorella pyrenoidosa using 96 h growth tests in a batch-culture system. Results showed that the growth of C. pyrenoidosa was significantly inhibited by [C8mim]Br, which would destroy the photosynthetic system II (PSII), and hinder the photosynthetic electron transfer of C. pyrenoidosa. By changing the photosynthetic pigments, C. pyrenoidosa attempted to repair the destruction of PSII system and capture more light quanta for photosynthesis. Moreover, remarkable physiological and biochemical responses, especially for the antioxidant enzymes in C. pyrenoidosa, occurred in [C8mim] Br treatments. [C8mim]Br increased total soluble protein content and enhanced antioxidant enzymes activities at low concentrations, but inhibited them at high concentrations. These observations indicated that moderate [C8mim]Br stress would stimulate the synthesis of proteins against stress and quenching of free radicals. And the general increase of MDA contents suggested that the physiological effects of [C8mim]Br were probably exerted through free radical generation. Thus, we suggest that it is necessary to evaluate influences of ionic liquids before their release into the natural environment in order to predict their impacts, avoiding irreparable damages.
Keywords
Ionic Liquid, Chlorella pyrenoidosa, 1-Octyl-3-Methylimidazolium Bromide ([C8mim]Br), Photosynthetic Activity, Antioxidant Enzymes
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