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Vol.4 , No. 3, Publication Date: Aug. 3, 2017, Page: 25-31
, indicating that the cytochrome (Cyt) pathway was mostly activated in their electron transport chain (ETC). Also, that most of these oxidations could donate their electrons to Cyt pathways within the ETC of <i> H. carnosa</i> mitochondria. In addition, the measurable respiration rate with individual substrate oxidations was less than the total measurable respiration rate with simultaneous substrate oxidations. These results confirm that <i> H. carnosa</i> mitochondria are more closely connected with Cyt pathway rather than alternative (Alt) pathway and <i> H. carnosa</i> mitochondrial ETC was not fully saturated by a single substrate oxidation.&picture=http://www.aascit.org/aascit/decorators/img/journal/903.jpg)
| [1] | Hoang Thi Kim Hong, Department of Biology, Hue University of Sciences, Hue City, Vietnam. |
This research investigated the simultaneous substrate oxidations and alternative respiration with succinate, NADH and NADPH in mitochondria from Hoya carnosa leaves. The result shows that H. carnosa mitochondria readily oxidized succinate with respiratory control and typical ADP/O ratios. The mitochondria also oxidized both NADH and NADPH with significant rates in the present of Ca2+ in which H. carnosa mitochondria rapidly oxidized NADH with higher respiration rates than those of NADPH oxidation. Both individual and simultaneous oxidations of succinate, NADH and NADPH in intact mitochondria were very sensitive with cyanide (KCN), indicating that the cytochrome (Cyt) pathway was mostly activated in their electron transport chain (ETC). Also, that most of these oxidations could donate their electrons to Cyt pathways within the ETC of H. carnosa mitochondria. In addition, the measurable respiration rate with individual substrate oxidations was less than the total measurable respiration rate with simultaneous substrate oxidations. These results confirm that H. carnosa mitochondria are more closely connected with Cyt pathway rather than alternative (Alt) pathway and H. carnosa mitochondrial ETC was not fully saturated by a single substrate oxidation.
Keywords
Alternative Respiration, H. carnosa Mitochondria, NADH, NADPH, Simultaneous Substrate Oxidation, Succinate
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