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International Journal of Biological Sciences and Applications

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Class 1 Integrase and Genetic Cassettes blaoxa and blatem among Multi-Drug Resistant Shigella Isolates in Costa Rica

International Journal of Biological Sciences and Applications
Vol.1 , No. 1, Publication Date: Jul. 7, 2014, Page: 24-27

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Authors

[1]	Kenia Barrantes , Nutrition and Infection Section, Instituto de Investigaciones en Salud (INISA), University of Costa Rica, San José, Costa Rica.
[2]	Luz Chacón , Nutrition and Infection Section, Instituto de Investigaciones en Salud (INISA), University of Costa Rica, San José, Costa Rica.
[3]	Melissa Solano , Nutrition and Infection Section, Instituto de Investigaciones en Salud (INISA), University of Costa Rica, San José, Costa Rica.
[4]	Rosario Achí , Nutrition and Infection Section, Instituto de Investigaciones en Salud (INISA), University of Costa Rica, San José, Costa Rica.

Abstract

Shigella is an important enteropathogen and the major cause of dysentery and diarrheal disease around the world. There are no studies related to the molecular detection of integrons among Shigella spp. isolates in Costa Rica. Here we are reporting for the first time the presence of class 1 integrase (intI-1) and extended spectrum β-lactamases genetic cassettes, blaOXA, and blaTEM among multidrug resistant Shigella isolates in Costa Rica. The antibiotic resistance pattern and presence of integrase class 1(intI-1) and genetic cassettes blaOXA and blaTEM were analyzed in 30 Shigella isolates (S. flexneri (83%) and S. sonnei (17%). Bacteria were collected at hospitals and cliniques in Costa Rica and exclusively from stools of individuals with diarrhea. from Costa Rica. Twenty nine out of 30 isolates were resistant to at least, one antibiotic. Multidrug resistance was observed in 26 out of 30 isolates (87%), and found to carry integrase class 1 (93%), and genetic cassettes blaOXA (76%) and blaTEM (7%)

Keywords

Integron, Shigella, Costa Rica

Reference

[01]	World Health Organization. 2001. Global strategy for containment of Antimicrobial Resistance. Available: http://whqlibdoc.who.int/hq/2001/WHO_CDS_CSR_DRS2001.1.pdf. Accesed 01 November 2012.
[02]	Achí, R.; Siles, X.; Mata, L.; Lindberg, A.A. (1996). Shigellae assays based on immunomagnetic separation in children from urban marginal communities of Costa Rica. J. Infect. 32, 211-218.
[03]	Barrantes, K.; Achí R.; Bolaños, S.; Cerdas, M.; Cortés, X. (2006). Calidad microbiológica y aislamiento de Shigella flexneri en vegetales frescos del Área Metropolitana de Costa Rica, 2001-2002. Avances de Investigación en Seguridad Alimentaria y Nutricional (SAN). NCAP, ME/11. 42-48.
[04]	Barrantes, K; McCoy C; Achí R. (2010). Detection of Shigella in lettuce by the use of a rapid molecular assay with increased sensitivity. Braz. J. Microbiol. 41(4), 993-1000.
[05]	Barrantes, K.; Veko, P.; Achí, R. (2004). Brote de diarrea asociado a Shigella sonnei debido a contaminación hídrica. San José, Costa Rica, 2001. Rev. Costarric. Cienc. Méd. 25, 15-22.
[06]	Valiente, C.; Mora, D. (2005). Estudio bacteriológico del agua asociado a brotes de diarrea en Costa Rica, 1999-2005. Rev. Colegio Microb. Quim. Clin. Costa Rica. 11(suppl), 11-14.
[07]	Niyogi S.K. (2005). Shigellosis. J Microbiol. 43(2), 133-143.
[08]	Ke X.; Gu B.; Pan S; Tong M. (2011). Epidemiology and molecular mechanism of integron-mediated antibiotic resistance in Shigella. Arch Microbiol. 193, 767-774.
[09]	Stokes, HW; Gillings MR. (2011). Gene flow, mobile genetic elements and the recruitment of antibiotic resistance genes into Gram-negative pathogens. FEMS Microbiol. Rev. 35, 790-819.
[10]	Mazel, D. (2006). Integrons: agents of bacterial evolution. Nature. 4, 608-620.
[11]	Poirel L.; Naas T.; Nordmann P. (2010). Diversity, Epidemiology, and Genetics of Class D (beta)-Lactamases. Antimicrob. Agents Chemother. 54(1), 24-38.
[12]	Frank T.; Gautier V.; Talarmin A.; Bercion R.; Arlet G.(2007). Characterization of sulphonamide resistance genes and Class 1 integron gene cassettes in Enterobacteriacea, Central African Republic (CAR). Antimicrob. Agents. Chemother. 59,742-745.
[13]	Muñoz J.; Bello H.; Domínguez M.; Mella S.; Zemelman R.;González G. (2003). Integrones y cassettes genéticos de resistencia a antimicrobianos en cepas de Shigella flexneri. Rev. Med.Chile. 131-137.
[14]	Peirano G.; Agerso Y.; Aarestrup F.M.;dos Prazeres Rodríguez D. (2005). Ocurrence of integrons and resistance genes among sulphonamide-resistant Shigella spp. from Brazil. J. Antimicrob. Chem. 55, 301-305.
[15]	Chang CY; Lu PL; Lin CC; Lee TM; Tsai MY and Chang L.(2011). Integron types, gene cassettes, antimicrobial resistance genes and plasmids of Shigella sonnei isolates from outbreaks and sporadic cases in Taiwan. J. Med. Microbiol. 60, 197-204.
[16]	DuPont. Chapter 57: Antimicrobial resistance of Shigella spp, typhoid Salmonella and nontyphoid Salmonella. In: D.L. Mayer (ed).Antimicrobial Drug Resistance. Humana Press, as part of Springer Science + Business Media. LLc 2009.825:831.