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Vol.3 , No. 6, Publication Date: Dec. 5, 2017, Page: 64-70
, Stool samples were obtained and assayed for rotavirus by the immunochromatography test (ICT), enzyme linked immunosorbent assay (ELISA) and quantitative real time (RT-PCR). 50 out of the 70 patients (71.42%) were positive for qr RT -PCR. Forty-five (64.28%) and 44 (62.85%) were positive for ICT and ELISA, respectively. There was a significant association between the severity of the disease as determined by the Vesikari score and rotavirus infection. The RT-PCR assay evaluated in the study was shown in general to have comparable performance for RVA detection in fecal samples. The real-time PCR assay is excellent tool for diagnostic laboratories, and the RT-PCR assay working in a portable PCR machine is highly sensitive and specific and shows promise for on farm molecular detection of RVA.&picture=http://www.aascit.org/aascit/decorators/img/journal/893.jpg)
| [1] | El-Sayed M. El-Morsy, Microbiology & Botany Department, Faculty of Science, Damietta University, Damietta, Egypt. |
| [2] | Maysaa E. Zaky, Pediatric Hospital, Faculty of Medicine, Mansoura University, Mansoura, Egypt. |
| [3] | Mahmoud A. Alhusseny, Microbiology & Botany Department, Faculty of Science, Damietta University, Damietta, Egypt. |
| [4] | Ahmed A. Abd Elfatah, Microbiology & Botany Department, Faculty of Science, Damietta University, Damietta, Egypt. |
| [5] | Ahmed S. El-Shafey, Microbiology Section, Faculty of Science, Tanta University, Tanta, Egypt. |
Rapid diagnosis assays have the advantage of facilitating the process of large amount of samples in a very short period of time, allowing fast management measure and treatment to reduce disease severity and infection spread. Rotavirus infections are a major cause of diarrhea in children in both developed and developing countries. Rotavirus genetics, patient immunity, and environmental factors are thought to be related to the severity of acute diarrhea due to rotavirus in infants and young children. Diarrhea remains the second most common cause of death among children below 5 years globally. Among various enteric pathogens, rotavirus appears to be the most important etiological agent of acute gastroenteritis in infants and young children. A total of 100 stool samples, this study included 70 children with AGE and 30 healthy control children. They were chosen from Mansoura Pediatric Hospital from November 2015 to March 2017. Rotavirus can be detected in stool samples by electron microscopy (EM), Stool samples were obtained and assayed for rotavirus by the immunochromatography test (ICT), enzyme linked immunosorbent assay (ELISA) and quantitative real time (RT-PCR). 50 out of the 70 patients (71.42%) were positive for qr RT -PCR. Forty-five (64.28%) and 44 (62.85%) were positive for ICT and ELISA, respectively. There was a significant association between the severity of the disease as determined by the Vesikari score and rotavirus infection. The RT-PCR assay evaluated in the study was shown in general to have comparable performance for RVA detection in fecal samples. The real-time PCR assay is excellent tool for diagnostic laboratories, and the RT-PCR assay working in a portable PCR machine is highly sensitive and specific and shows promise for on farm molecular detection of RVA.
Keywords
(AGE) Acute Gastroenteritis, Vesikari Score, (RT-PCR)
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