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Vol.3 , No. 4, Publication Date: Aug. 29, 2017, Page: 37-44
 is an acute viral disease of goats and sheep. The disease causes a huge loss of small ruminant production per year in Bangladesh. Diagnosis of PPR from suspected field samples is not properly carried out due to unreliable transportation and poor infrastructure. This study was planned to develop and validate a hazard free, time saving and specific sampling technique for the diagnosis of PPRV (Peste des petits ruminants vaccine). Two categories of samples including bronchial lymph node and viral suspensions from nasal swab (from dead and live animals) were collected from 25 goats and from each case corresponding nasal swab smeared filter paper was also taken as sample. All the samples were subjected to RT-PCR with (lymph node and viral suspension from nasal swab) or without (smeared filter paper) RNA extraction using N gene specific primer. Twenty three goats found PPR positive for both categories of samples. One found positive where extracted RNA is used as template but negative to smeared filter paper RNA. On the other hand, one found negative to extracted RNA but positive to filter paper RNA. One goat found negative for both the cases. The result of the study has suggested that sampling through filter paper could be easiest, less time consuming and hazardless for transportation of samples from the field through which diagnosis of PPRV can be done by RT-PCR.&picture=http://www.aascit.org/aascit/decorators/img/journal/893.jpg)
| [1] | Md. Tarek Hossain, Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh. |
| [2] | Rahman M. M., Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh. |
| [3] | Begum J. A., Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh. |
| [4] | Haque M. E., Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh. |
| [5] | Habib M. A., Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh. |
| [6] | Islam M. R., Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh. |
| [7] | Emdadul Haque Chowdhury, Department of Pathology, Bangladesh Agricultural University, Mymensingh, Bangladesh. |
Peste des petits ruminants (PPR) is an acute viral disease of goats and sheep. The disease causes a huge loss of small ruminant production per year in Bangladesh. Diagnosis of PPR from suspected field samples is not properly carried out due to unreliable transportation and poor infrastructure. This study was planned to develop and validate a hazard free, time saving and specific sampling technique for the diagnosis of PPRV (Peste des petits ruminants vaccine). Two categories of samples including bronchial lymph node and viral suspensions from nasal swab (from dead and live animals) were collected from 25 goats and from each case corresponding nasal swab smeared filter paper was also taken as sample. All the samples were subjected to RT-PCR with (lymph node and viral suspension from nasal swab) or without (smeared filter paper) RNA extraction using N gene specific primer. Twenty three goats found PPR positive for both categories of samples. One found positive where extracted RNA is used as template but negative to smeared filter paper RNA. On the other hand, one found negative to extracted RNA but positive to filter paper RNA. One goat found negative for both the cases. The result of the study has suggested that sampling through filter paper could be easiest, less time consuming and hazardless for transportation of samples from the field through which diagnosis of PPRV can be done by RT-PCR.
Keywords
Peste Des Petits Ruminants (PPR), Filter Paper, RT-PCR, N Gene, Sequencing
Reference
| [01] | Couacy-Hymann E, Roger F, Hurard C, Guillou JP, Libeau G, Diallo A. Rapid and sensitive detection of peste des petits ruminants virus by a polymerase chain reaction assay. J. Virol. Meth. 2002; 100: 17-25. |
| [02] | Sil BK, Rahman MM, Taimur MJFA, Sarker AJ. Observation of outbreaks of PPR in organized goat farms and its control strategy. Presented at the Ann. Con. of the Bangl. Soc. of Vet. Edu. and Res, 3 December 1995, BARC, Dhaka. |
| [03] | Islam MR, Shamsuddin M, Das PM, Dewan ML. An outbreak of peste des petits ruminants in Black Bengal goats in Mymensingh, Bangladesh. The Bangl. Vet. 2001; 18: 14-19. |
| [04] | Lefevre PC, Diallo A, Schenkel F, Hussein S, Staak G. Serological evidence of peste des petits ruminants in Jordan. Vet. Record. 1991; 128: 110. |
| [05] | Abu Elzein EME, Hassanien MM, Alfaleg AIA, Abd Elhadi MA, Housawi FMT. Isolation of PPR virus from goats in Saudi Arabia. Vet. Rec. 1990; 127: 309-310. |
| [06] | Shaila MS, Purushothaman V, Bhavasar D, Venugopal K, Venkatesan RA. Peste des petits ruminants of sheep in India. Vet. Rec. 1989; 125: 602. |
| [07] | Kamaruddin KM, Islam MR. Transboundry diseases in Bangladesh: PPR and rinderpest. Proceedings of Eleventh BSVER (Bangl. Soc. of Vet. Edu. And Res.). Ann. Sci. Con., Bangladeesh Agricultural University, Mymensingh, Bangladesh. 2005; 19-20. |
| [08] | Khan HA, Siddique M, Arshad MJ, Khan QM, Rehman SU. Sero- revalence of Peste des petits ruminants (PPR) Virus in sheep and goats in Punjab province of Pakistan. Pakistan Vet. J. 2005; 27: 109-112. |
| [09] | Bellini WJ, Rota JS, Rota PA. Virology of measles virus. J. Infect. Dis. 1994; 170: 515-523. |
| [10] | Ozturk G. Pest of small ruminants (Peste des petits ruminants). Saglik Bilimleri Dergisi, Fisat Universitesi, 1999; 13: 201-204. |
| [11] | Sinnathamby G, Renukaradhya GJ, Rajashekhar M, Nayak R, Shaila MS. Immune responses in goats to H-N glycoprotein of PPRV: identification of a T- cell determinant. Vac. 2001; 19: 4816-4823. |
| [12] | Gibbs EP, Taylor WP, Lawman MJ, Bryant J. Classification of peste des petits ruminants virus as the fourth member of the genus Morbillivirus. Intervirol. 1979; 11: 268-274. |
| [13] | Shaila MS, Shamaki D, Forsyth AM, Diallo A, Goatley L, Kitching RP, Barrett T. Geographic distribution and epidemiology of peste des petits ruminants viruses. Virus Res. 1996; 43: 149-153. |
| [14] | Ozkul A, Akca Y, Alkan F, Barrett T, Karaoglu T, Dagalp SB, Anderson J, Yesilbag K, Cokcaliskan C, Gencay A, Burgu I. Prevalence, distribution, and host range of Peste des petits ruminants virus, Turkey. Emerg. Infect. Dis. 2002; 8: 708-712. |
| [15] | Kwiatek O, Minet CG, Hurardy CC, Carlssonz E, Karimovz B, Albina E, Diallo A, Libeau G. Peste des Petits Ruminants (PPR) Outbreak in Tajikistan. J. Comp. Path. 2007; 136: 111-119. |
| [16] | Wang Z, Bao J, Wu X, Liu U, Li L, Liu C, Suo L, Xie Z, Zhao W, Zhang W, Yang N, Li J, Wang S, Wang J. Peste des Petits Ruminants Virus in Tibet, China. Emerg. Infect. Dis. 2009 15: 299–301. |
| [17] | Balamurugan V, Sen A, Saravanan P, Singh RP, Singh RK, Rasool TJ, Bandyopadhyay SK. One-step Multiplex RT-PCR Assay for the Detection of Peste des petits ruminants Virus in Clinical Samples. J. Vet. Res. Commun. 2006; 30: 655-666. |
| [18] | Prado I, Rosario D, Bernardo L, Alvarez M, Rodriguez R, Vazquez S, Guzman MG. PCR detection of dengue virus using dried whole blood spotted on filter paper. J. Virol. Meth. 2005; 1: 75–81. |
| [19] | Yourno J, Conroy J. A novel polymerase chain reaction method for detection of human immunodeficiency virus in dried blood spots on filter paper. J. Clin. Microbiol. 1992; 30: 2887–2892. |
| [20] | Pitcovsky J, Shmueli E, Krispel S, Levi N. 1999. Storage on filter paper for genetic analysis. J. Virol. Meth. 1999; 83: 21-26. |
| [21] | Kailash U, Hedau S, Gopalkrishan V, Katiyar S, Das BC. A simple 'paper smear' method for dry collection, transport and storage of cervical cytological specimens for rapid screening of HPV infection by PCR. J. Med. Microbiol, 2002; 51: 606-683. |
| [22] | Michaud A, Gil P, Kwiatek O, Prome S, Dixon L, Romero L, Le Potier MF, Arias M, Couacy-Hymann E, Roger F, Libeau G, Albina E. Long-term storage at tropical temperature of dried-blood filter papers for detection and genotyping of RNA and DNA viruses by direct PCR. J. Virol. Methods. 2007; 146: 257-265. |
| [23] | Li CC, Beck IA, Seide KD, Frenkel LM. Persistence of Humanimmun odeficiency virus type 1 subtypes B DNA in dried-blood samples on FTA filter paper. J. Clin. Microbiol. 2004; 42: 3847–3849. |
| [24] | Chaisomchit S, Wichajarn R, Janejai N, Chareonsiriwatana W. Stability of genomic DNA in dried blood spots stored on filter paper. South Asian J. Trop. Med. Pub. Hlth. 2005; 36: 270–273. |
| [25] | Rahman M A, Shadmin I, Noo M, Parvin R, Chowdhury EH, Islam MR. Peste des petits ruminants virus infection of goats in Bangladesh: Pathological investigation, molecular detection and isolation of the virus. The Bangladesh Veterinarian. 2011; 28 (1): 1-7. |
| [26] | Ularamu HG, Owolodun OA, Woma TY, Audu BJ, Aaron GB, Chollom SC, Shamak D. Molecular diagnosis of recent suspected outbreaks of peste des petits ruminants (PPR) in Yola, Adamawa State, Nigeria. African Journal of Biotechnology. 2012; 11 (5): 1158-1162. |
