ISSN Print: 2381-1013  ISSN Online: 2381-1021
American Journal of Agricultural Science  
Manuscript Information
Assessment of Chitosan as Preservative on Shelf Life and Major Nutrient Contents on Fruits and Vegetables
American Journal of Agricultural Science
Vol.6 , No. 1, Publication Date: Mar. 6, 2019, Page: 1-10
956 Views Since March 6, 2019, 576 Downloads Since Mar. 6, 2019

Must Alima Rahman, Department of Agricultural Chemistry, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh; Institute of Radiation and Polymer Technology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh.


Mohammed Ariful Islam, Department of Agricultural Chemistry, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.


Mohammad Tipu Sultan, Department of Soil, Water and Environment Discipline, Khulna University, Khulna, Bangladesh.


Abu Hena Mostofa Kamal, Institute of Radiation and Polymer Technology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh.


Ruhul Amin Khan, Institute of Radiation and Polymer Technology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh.


Mohammad Razzak, Institute of Radiation and Polymer Technology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh.


Mohammad Tazul Islam Chowdhury, Department of Agricultural Chemistry, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.


Mohammad Sirajul Islam Khan, Department of Agricultural Chemistry, Sher-e-Bangla Agricultural University, Dhaka, Bangladesh.


Mohammad Zahirul Islam Mollah, Institute of Radiation and Polymer Technology, Bangladesh Atomic Energy Commission, Dhaka, Bangladesh.


Postharvest loss of perishable fruits and vegetable is of great concern for Bangladesh. As agricultural crops are rapidly perishable, they are also damaged due to high humidity and temperature. To address this problem, chitosan was prepared from prawn shell collected from local market and applied on tropical fruits and vegetables namely banana, tomato and papaya for extending shelf life. Samples were collected from 3 markets near Dhaka city. Chitosan was applied on treatment groups by deep coating method. The experiment was laid out with three replications under two doses of chitosan (500ppm and 1000ppm). Significant difference were found higher in tomato and papaya than banana for color index and weight loss parameter, while EC, pH, percent Total Suspended Solids (TSS) and Total Dissolved Solids (TDS) levels were remains similar. In case of Total Viable Count (TVC) and Total Fungal Count (TFC), the significant difference was higher in control than 500ppm and 1000ppmchitosan for banana, tomato and papaya. Percentages of nitrogen (N) and potassium (K) content were remaining similar or high, while phosphorus (P) content was remaining similar. Present study shows that 500ppm dose of chitosan is more effective compared to that of 1000ppm dose of chitosan in banana. It can be concluded that coating with chitosan can be employed to extend the shelf life and to improve quality of fruits and vegetables by delaying ripening, reducing weight loss and reducing microbial growth in fruits and vegetables.


Chitosan, Preservatives, Deep Coating Method, Shelf Life, Nutrient Contents


BBS (Bangladesh Bureau of Statistics), 2016. Yearbook of Agricultural Statistics – 2015, 27th Series, Statistics and Informatics Division (SID), Ministry of Planning Government of the People’s Republic of Bangladesh, 3 pp.


Croisier, F. and Jerome, C. 2013. Chitosan-based biomaterials for tissue engineering, 49 (4): 780-792.


Gomes, L. P., Paschoalin, V. M. F. and Del Aguila, E. M. 2017. Chitosan Nanoparticles: Production, Physicochemical Characteristics and Nutraceutical Applications, 9 (1): 387.


Roberts, G. A. F. 1997. Chitosan production routes and their role in determining the structure and properties of the product. In Advances in Chitin Science, National Taiwan Ocean University, Taiwan, vol. 2: 22–31.


Win, N. N., Stevens, W. F. 2001. Shrimp chitin as substrate for fungal chitin deacetylase. Appl Microbiol Biotechnol, 57: 334–341.


Ravi Kumar, M. N. V. 2000. A review of chitin and chitosan applications. Reactive Functional Polym, 46: 1–27.


Marguerite, R. 2006. Chitin and chitosan: properties and applications. Progress in PolymSci, 31: 603–632.


Virginia, E., Marie, G., Eric, P. and Luc, A. 2011. Structure and properties of glycerol plasticized chitosan obtained by mechanical kneading. Carbohydrate Polym, 83: 947–952.


Liu, M., Zhang, Y., Wu, C., Xiong, S. and Zhou, C. 2012. Chitosan/halloysite nanotubes bionanocomposites: structure, mechanical properties and biocompatibility. Int J Biological Macromol, 51: 566–575.


Bae, K. P. and Moo-Moo, K. 2010. Applications of chitin and its derivatives in biological medicine. Int J Mol Sci, 11: 5152–5164.


Ramya, R., Venkatesan, Jayachanndran Kim, S. and Sudha, P. N. 2012. Biomedical applications of chitosan: an overview. J Biomaterial Tissue Engg, 2: 100–111.


Bansal, V., Pramod, K. S., Nitin, S., Omprakask, P. and Malviya, R. 2011. Applications of chitosan and chitosan derivatives for drug delivery. AdvaBiol Res, 5: 28–37.


Valerie, D. and Vinod, D. V. 1998. Pharmaceutical applications of chitosan. Pharmaceutical SciTechnol Today, 1: 246–253.


Hafdani, F. N. and Sadeghinia, N. 2011. A review on applications of chitosan as a natural antimicrobial. World Academy of SciEnggTechnol, 50: 252–256.


Rashidul Alam., Mubarak A. Khan., Ruhul A. Khan., Sushanta Ghoshal. and M. I. H. Mondal. 2008. Study on the Physico-mechanical Properties of Photo-cured Chitosan Films with Oligomer and Acrylate Monomer. J Polym Environ, 16: 213–219.


Yu, Wang., Baogang Wang. and Lite, Li. 2008. Keeping quality of tomato fruit by highelectrostatic field pretreatment duringstorage. J Sci Food Agric, 88: 464–470.


Zhang Juan., Zhang Shaoying., Yu Youwei. And Ren Yinzhe. 2014. Cherry tomato preservation using chitosan combined with zinc/cerium ion, 3 (2): 111-118.


USDA (United States Department of Agriculture). 2004. Soil survey laboratory manual. Soil survey investigation report no. 42, version 4.0, USDA-NRCS, Nebraska, USA.


Jackson, M. L. 1973. Soil chemical analysis. Prentice Hall of India Pvt. Ltd. New Delhi, pp. 495-498.


Misra, R. D. and Ahmed, M. 1987. Manual of Irrigation Agronomy. Oxford and IBH publishing company and private limited, New Delhi, India, pp. 248-271.


International Commission on Microbial Specifications for Food. 1978. Microorganisms in foods. Their significance and methods of enumeration, 2nd ed. University of Toronto Press, Toronto.


Anderson, E. H. and C. A. Stuart. 1935. A quantitative differential method for counting mixed cultures of bacteria. J. Bacteriol, 30: 207–209.


Reed, R. W. and G. B. Reed. 1948. Drop plate method of counting viable bacteria. Can. J. Res. E: 316–326.


Dick, S. L. 1967. The enumeration of bacteria in urinary tract infections. Can. J. Med. Techn, 29: 15–22.


Guthrie, R. K. and D. J. Reeder. 1969. Membrane filter- fluorescent antibody method for detection and enumeration of bacteria in water. Appl. Microbiol, 17: 399–401.


Winn, W. R., M. L. White, W. T. Carter, W. T. Miller, and S. M. Finegold. 1966. Rapid diagnosis of bacteremia with quantitative differential membrane filtration culture. J. Am. Med. Assoc, 197: 539–548.


Jackson, M. L. 1962. Soil Chemical Analysis. Prentice hall, In: Englewood cliffs, New Jersey, USA.


Gomez, K. A. and Gomez, A. A. 1984. Statistical Procedures for Agricultural Research. 2nd ed. International Rice Research Institute, Los Banos, Philippines, p. 627.


Chowdhury, M. A., Khairun, Y., Salequzzaman, M. and Rahman, M. M. 2011. Effect of combined shrimp and rice farming on water and soil quality in Bangladesh. Aquaculture International, 19 (6): 1193-1206.


Sumner, M. 2000. Handbook of soil science. CRC Press, Boca Raton, pp. 2148.

  Join Us
  Join as Reviewer
  Join Editorial Board