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Vol.4 , No. 3, Publication Date: Jun. 7, 2017, Page: 29-36
 Using SDS-PAGE Analysis&description=Wheat is the most essential food crop and taxonomically belongs to family poaceae. Bread wheat is a Hexapliod species with 2n=6x=42 chromosomes having high genetic diversity, the genetic variation enables plant species to adapt to changing environments and provide resistance against different diseases. The scientists are highly interested to study the genetic diversity among wheat varieties morphologically (plant height, tiller number, spikes number, main ear length, spike lets number, grain number, grain weight, seed hardness, sedimentation value, and grain yield etc) biochemically (phenol color reaction and through different other chemical substance) and at molecular level (protein content, RNA and DNA variation). It has been revealed that morphological and biochemical variations are correlated to environmental condition (stress and favorable condition). The simple SDS-PAGE (Sodium dodecyl sulfate -polyacrylamide gel electrophoresis) method was adopted to show genetic variation in wheat which helps in classification and making a dendrogam with different cluster groups, that is vital for both plant breeder and germplasm curators. The high molecular weight-gultenin subunits (HMW-GS) and low molecular weight-gultenin subunit (LMW-GS) were analazyed through SDS-PAGE techniques. The variation in glutenin subunits was due to different allelic combination in various loci in different chromomes the glutein have important baking qualities. This study was aim to collect information related to wheat genetic diversity and the use of SDS-PAGE study of wheat endosperms protein that is valuable for assessment of genetic variability and cultivars identification that help in wheat breeder programs.&picture=http://www.aascit.org/aascit/decorators/img/journal/892.jpg)
| [1] | Naeem Khan, Department of Plant Sciences, Quaid-e-Azam University, Islamabad, Pakistan. |
| [2] | Shahid Ali, Department of Botany, Hazara University, Mansehra, Pakistan. |
Wheat is the most essential food crop and taxonomically belongs to family poaceae. Bread wheat is a Hexapliod species with 2n=6x=42 chromosomes having high genetic diversity, the genetic variation enables plant species to adapt to changing environments and provide resistance against different diseases. The scientists are highly interested to study the genetic diversity among wheat varieties morphologically (plant height, tiller number, spikes number, main ear length, spike lets number, grain number, grain weight, seed hardness, sedimentation value, and grain yield etc) biochemically (phenol color reaction and through different other chemical substance) and at molecular level (protein content, RNA and DNA variation). It has been revealed that morphological and biochemical variations are correlated to environmental condition (stress and favorable condition). The simple SDS-PAGE (Sodium dodecyl sulfate -polyacrylamide gel electrophoresis) method was adopted to show genetic variation in wheat which helps in classification and making a dendrogam with different cluster groups, that is vital for both plant breeder and germplasm curators. The high molecular weight-gultenin subunits (HMW-GS) and low molecular weight-gultenin subunit (LMW-GS) were analazyed through SDS-PAGE techniques. The variation in glutenin subunits was due to different allelic combination in various loci in different chromomes the glutein have important baking qualities. This study was aim to collect information related to wheat genetic diversity and the use of SDS-PAGE study of wheat endosperms protein that is valuable for assessment of genetic variability and cultivars identification that help in wheat breeder programs.
Keywords
Hexopliod, SDS-PAGE, HMW-GS, LMW-GS, Glutenin
Reference
| [01] | Cromwell, E., Cooper, D., Mulvany, P., 1999. Agricultural Biodiversity and Livelihood: issues and entry points: Paper for DFID Linking Policy and Practice on Biodiversity Project (LPPB), ODI, FAO, ITDG. |
| [02] | Browning, J. A., 1988. Current thinking on the use of biodiversity to buffer small grains against highly epidemic and foliar pathogens: problems and future prospects.” P. 76-90 IN CIMMYT, Breeding strategies for resistance to the rust of wheat. Mexico. D. F. CIMMYT. |
| [03] | Clay, J., 1991. Cultural survival and conservation: Lessons from the past 20 years in: Common Wealth of Australia: Biodiversity series paper No.1. deptt. of Environment, Sports Territories, Canberra. |
| [04] | FAO., 1996. Report of the International Technical Conference on Plant Genetic Resources. Leipzing Germany. |
| [05] | Miller, T. E., Koebner, R. M. D., (eds).2000. Proceedings of the Seventh International Wheat Genetic Symposium, Cambridge. Institute of Plant Sciences Research, Cambridge. |
| [06] | Kerby, K., J. Kuspira., 1987. The phylogeny of the polyploid wheats Triticum aestivum (bread wheat) and Triticum turgidum (macaroni wheat)." Genome29.5: 722-737. |
| [07] | Lagudah, E. S., Dubcovsky, J., Powell, W., 2001. Wheat genomics. Plant Physiol. Biochem. 39: 335-344. |
| [08] | Hassan, M., Wasiullah, Iqbal, J., Ilyas, M., 2003. Evaluation of Wheat Varieteis under the Agro-climate Condition of Barani Agricultural Research Station, Kohat. Pak. J. Agron. 2(1): 8-12. |
| [09] | Smith, J. S. C., Smith, O. S., 1989. The description and assessment of distances between inbred lines of maize: the utility of morphological, biochemical and genetic descriptors and scheme for testing of distinctiveness between inbred lines. Maydica. 34:151-161. |
| [10] | Amurrio, J. M., De Ronand, A. A., Zeven, A. C., 1995. Numerical taxanomy of Iberian pea landraces based on quantitative and qualitative characters. Euphytica. 82:195-205. |
| [11] | Van-Beuningen, L. T., Busch, R. H., 1997. Genetic diversity among North American Spring. |
| [12] | Weltzien, El., Fischbeck, G., 1990. Performance and Variability of Local Barley Landraces in Near‐Eastern Environments." Plant Breeding 104.1: 58-67. |
| [13] | Anwar, R., 1993. Distribution of wild and primitive wheats in Pakistan. Biodiversity and Wheat Improvement. Damania, A. B. (ed.) A Wiley-Sayce Publication. Pp. 417-422. |
| [14] | Ciaffi, M., Lafiandra, D., Porceddu, E., Benedettelli, S., 1993. Storage protein variation in wild emmer wheat (T. turgidum spp. Dicoccoides) from Jordan and Turkey. I. Electrophoretic characterization of genotypes. Theor. Appl. Genet. 86: 474-480. |
| [15] | Tahir, M., Turchetta, T., Anwar, R., Lafiandra, D., 1996. Assessment of genetic variability in hexaploid wheat land races of Pakistan based on polymorphism for HMW glutenin subunits. Genet. Resources and Crop Evolution. 43: 211-220. |
| [16] | Fabrizius, M. A., Busch, R. H., Khan, K., Huckle, L., 1998. Genetic diversity and heterosis of spring wheat crosses. Crop Sci. 38:1108-1112. |
| [17] | Shah, M. M., Baenziger, P. S., Yen, Y., Gill, K. S., Moreno-Sevilla, B., Haliloglu, K., 1999. Genetic analysis of agronomic traits controlled by wheat chromosome 3A. Crop Sic. 39: 1016-1021. |
| [18] | Moghaddam, M., Ehdiae, B., Waines, J. G., 1998. Genetic variation for and interrelationships among agronomic traits in land races of bread wheat from southwestern Iran. J. Genet. & Breed. 52: 73-81. |
| [19] | SA., Elizabeth, AV., Schammacs, E. A., Vencovsky, R., Martin, P. S., Bandel, G., 2001. Germplasm characterization of Sasbania accessions based on multivariate analysis. Genetic Resource and Crop Evolution. 48: 79-90. |
| [20] | Zhu, J., Huang, S., Khan, K., Brien, L. O., 2001. Relationship of protein quantity, quality and Dough Properties with Chinese Steamed Bread Quality. J. Cere. Sci. 33(2): 205-212. |
| [21] | Dotlacil, L., Gregova, E., Hermuth, J., Stehno, Z., Kraic, J., 2002. Diversity of HMW Glu alleles and evaluation of their effects on some characters in winter wheat land races and old cultivars. Czech Journal of Genetics and Plant Breeding. 38 (3-4): 109-116. |
| [22] | Gupta, R. S., Tiwari, D. K., Doel, S. S., Singh, R. P., 2002. Genetic divergence in bread wheat (Triticum aestivum L. em Thell). New Botanist. 60:237-243. |
| [23] | Jagdish, R., Harsh, C., 2002. Rate of grain growth in advanced wheat (Triticum aestivum) accession under late sown environment. Indian J. Agri. Sciences. 72(10): 581-585. |
| [24] | Khan, M. F., Schumann, E., Weber, W. E., 2002. Characterization of Pakistani wheat varities for General Cultivation in the Mountanious Regions of Azad Kashmir. Asian J. Plant. Sci. 1(6): 699-702. |
| [25] | Kumar, P., Shukla, R. S., 2002. Genetic analysis for yield and its attributed traits in bread wheat under various situation. JHKVV research journal 2000, 36 (1-2): 95-97. |
| [26] | Singh, P., Mishra, P. K., Billore, M. M., Bhawsar, R. C., 2002. Studies on genetic diversity in Wheat (Triticum aestivum) Adv. In plant Sci. 15 (1): 301-305. |
| [27] | Anwar, R., Masood, S., Khan, M. A., Nasim, S., 2003. The high molecular weight glutenin subunit composition of wheat (Triticum aestivum L.) land races from Pakistan. Pak. J. Bot. 35(1): 61-68. |
| [28] | Payne, P. I., 1987. Genetics of wheat storage proteins and the effect of allelic variation on bread making quality. Ann. Rev. Plant Physiol. 38: 141-153. |
| [29] | Bhattacharya, M., Jafari Shabestari, J., Qualset, C., Corke, H., 2003. Diversity of protein quality traits. Cereal research communications. 31: 193-200. |
| [30] | Jan, I., Usman, M., Iftikhar., Khalil, H., Jan, T., 2003. Performance of recently released wheat cultivars. Asian J. Pl. Sci. 2(8): 627-632. |
| [31] | Kamal, A. M. A., Islam, M. R., Chowdhury, B. L. D., Talukder, M. A. M., 2003. Yield performance and Grain Quality of Wheat Varieties Grown under Rain fed and Irrigated Conditions. Asian J. Pl. Sci. 2(3): 258-260. |
| [32] | Khan, M. F., 2003. Evaluation of Hexaploid Wheat Genotypes by using DNA isolation and gel-electrophoresis. Asian J. Plant. Sci 2(2): 212-215. |
| [33] | Lad, D. B., Banger, N. D., Bhor, T. J., Mukhekar, G. D., Birader, A. B., 2003. Correlation and path coefficient in wheat. J. Maharashtra Agric. Univ. 28(1): 23-25. |
| [34] | Pawar, S. V., Patil, S. C., Naik, R. M., Jambhale, V. M., (2003). Genetic variability and heritability in wheat. Jour. Maharashtra Agric. Uni. 27(3): 324-325. |
| [35] | Upadhyaya, H. D., 2003. Phenotypic diversity in groundnut (Arachis hypogaea L.) Core collection assessed by morphological and agronomical evaluations. Genetic Resources and Crop Evolution. 50: 539-550. |
| [36] | Renato, D. O., Masci, S., 2004. The low-molecular-weight glutenin subunits of wheat gluten. J. Cere. Sci. 39(3): 321-339. |
| [37] | Jakubauskiene, L., Juodeikiene, G., 2005. The relationship between protein fractions of wheat gluten and the quality of Ring-Shaped Rolls Evaluated by the Echolocation Method. Food Technol. Biotechnol. 43(3): 247-253. |
| [38] | Li, Q. Y., Yan, Y. M., Wang, A. L., Zhang, Y. Z., Hsam, S. L. K., Zeller, F. J., 2006. Detection of HMW glutenin subunit variations among 205 cultivated emmer accessions (T.turgidium ssp. Dicoccum). Plant breeding 125 (2): 120-124. |
| [39] | Popa, M., Gregova, E., Kraic, J., newsletter, P.G.R., 2006. Romanian wheat (T. aestivum L.) landraces characterized by seed storage-protein electrophoresis. (135): 53-58. |
| [40] | Xueli, A., Qiaoyun, L., Yueming, Y., Yinghua, X., Hsam, S. L. K., Zeller, F. J., 2005. Genetic diversity of European spelt wheat (Triticum aestivum ssp. spelta L. em. Thell.) Revealed by glutenin subunit variations at the Glu-1 and Glu-3 loci. Euphytica. 146 (3): 193-201. |
| [41] | Gaines, C., Reid, S., Vander Kant, J. F., Morris, C. F., 2006. Comparison of methods for gluten strength assessment. Cereal chemistry, 83 (3), 284-286. |
| [42] | Al Khanjari, S., Hammer, K., Buerkert, A., Röder, M. S. 2007. Molecular diversity of Omani wheat revealed by microsatellites: II. Hexaploid landraces. Genetic Resources and Crop Evolution, 54(7), 1407-1417. |
| [43] | Shuaib, M., I. Khan, Z. Ali, T. Ahmad and A. Zeb (2007). Evaluation of different wheat varieties by SDS-PAGE. Pak. J. Bio. Sci. 10(10): 1667-1672. |
| [44] | Wiser, H., 2007. Chemistry of Gluten Proteins. Food Microbial. 24(2): 115-119. |
| [45] | Caballero, L., Martin, M. A., Alvarez, J. B., 2008. Genetic diversity for seed storage proteins in Lebanon and Turkey populations of wild diploid wheat (Triticum urartu). |
| [46] | Martin, M. A., Martin, L. M., Alvarez, J. B., 2008. Polymorphism at the Gli-Au1 and Gli-Au2 loci in wild diploid wheat (Triticum urartu). Euphytica 163:303-307. |
| [47] | Eivazi, A. R., Naghavi, M. R., Hajheidari, M., Pirseyedi, S. M., Ghaffari, M. R., Mohammadi, S. A., Majidi, I., Salekdeh, G. H. and Mardi, M., 2008. Assessing wheat (Triticum aestivum L.) genetic diversity using quality traits, amplified fragment length polymorphisms, simple sequence repeats and proteome analysis. Annals of Applied Biology, 152(1), pp. 81-91. |
| [48] | Shewry., Peter, R., Underwood, C., Wan, Y., Lovegrove, A., Bhandari, D., Toole, G., Clare Mills, E. N., Denyer, K., Rowan, A. C. M. l., 2009. Storage product synthesis and accumulation in developing grains of wheat." Journal of Cereal Science 50, 1: 106-112. |
| [49] | Nisar, M., Khan, N., Ahmad, Z., Ghafoor, A., 2011. Genetic diversity and disease response of rust in bread wheat collected from Waziristan Agency, Pakistan." Int. J. Biodivers. Conserv. 3(1):10-18 |
| [50] | Zhang., Xiaofei., Jin, H., Zhang, Y., Liu, D., Li, G., Xia, X., He, Z., Zhang, A., 2012. Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat." BMC plant biology 12, 1: 1. |
| [51] | Hashjin, R. M., Fotokian, M. H., Sarbrzeh, M. A., Mohammadi, M., Talei, D., 2014. Genetic diversity of durum wheat genotypes using morpho-protein analysis." Cereal Research Communications 42, 4: 677-686. |
| [52] | Bietz J. A., Wall J. S., 1980. Identity of high molecular weight gliadin and ethanol-soluble glutenin subunits of wheat: relation to gluten structure. Cereal Chem. 1; 57(6):415-21. |
| [53] | Li, Q. Y., Y. M. Yan, A. L., Wang, X. L. A, Y. Z. Zhang, S. L. K Hsam and F. J. Zeller. 2006. Detection of HMW glutenin subunit variations among 205 cultivated emmer accessions (T.turgidium ssp. Dicoccum). Plant breeding 125 (2): 120-124. |
| [54] | Benmoussa, M., L. P. Vezina, M. Page, S. Yelle, S. Laberge., 2000. Genetic polymorphism in low-molecular-weight glutenin genes from Triticum aestivum, variet Chinese Spring. Theor. Appl. Genet. 100 (5): 789-793. |
| [55] | Yahata, E., W. M. Funatsuki, Z. Nishio, Y. Yamamoto, A. Hanaoka, H. Sugiyama, M. Tanida and H. Saruyama. 2006. “Relationship between the dough quality and content of specific Glutenin Proteins in Wheat Mill Streams, and Its Application to Making Flour Suitable for Instant Chinese Noodles”, Biosci. Biotechnol. Biochem. 70: 788-797. |
