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International Journal of Chemical and Biomedical Science

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A Fast and Simple Quantitative Profiling Method of Endogenous Saccharides in Rat Urine Using HILIC-MS/MS

International Journal of Chemical and Biomedical Science
Vol.4 , No. 4, Publication Date: May 30, 2018, Page: 54-59

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Authors

[1]	Gang Cheng, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
[2]	Bangjie Zhu, Department of Chemistry, East China Normal University, Shanghai, China.
[3]	Feng Liu, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
[4]	Yan Wang, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
[5]	Yu Cheng, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.
[6]	Chao Yan, School of Pharmacy, Shanghai Jiao Tong University, Shanghai, China.

Abstract

To explore the metabolism of endogenous saccharides in rat, we developed a simple, fast, simultaneously quantitative method for the endogenous saccharides in rat urine, using the hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry. In order to quantify 15 endogenous saccharides in rat urine, various conditions, including columns, chromatographic conditions, MS conditions, and urine preparation methods, were investigated and optimized. The reproducibility, precision, recovery, and stability of the method were verified. The results indicated that 5 times volume of cold organic solvents (methanol/acetonitrile, v/v, 50/50) coupled with vortex for 1 min and incubated at -20C for 20 min were the most optimal conditions for extraction. The results, according to the linearity, precision, recovery, matrix effect and stability, showed that the method was satisfactory in the quantification of endogenous saccharides in rat urine. Meeting the requirement of quantification in specific expanded metabolomic studies, the quantified analysis of endogenous saccharides in rat urine performed excellently in the sensitivity, high throughput and simple sample preparation.

Keywords

Endogenous Saccharides, Hydrophilic Interaction Chromatography, HILIC-UPLC-MS/MS, Rat Urine

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