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Vol.3 , No. 5, Publication Date: Sep. 8, 2017, Page: 73-78
. The result of the FTIR analysis showed the functional groups present in the adduct as follows: The band at 2948.85 cm<sup>-1</sup> was assigned to aldehyde (HC=O) group. The ones at 3850.5 cm<sup>-1</sup> 3736.73 and 3617.47 were found to be (O-H) stretch for water of hydration and N-H stretches. The sharp 2356.87 band was found to be due to N-H stretch from amines in the adduct while the bands at 1739.68, 1547.01 cm<sup>-1</sup> and 1032.63 cm<sup>-1</sup> were attributed to C=O and C-H stretch as well as C-N stretch due to tertiary amines respectively. The Antimicrobial screening test on the adduct also showed some antimicrobial effect to some micro- organism such <i>as Bacillus sustilis (12.0 mm), Proteum mirabilis (16.0 mm) and Canidad albicans (14.0 mm)</i> as their growth was inhibited when placed around their growth media. It was however found to have no effect on some fungi and algae such as <i>Escherichia coli and Staphylococcus aureus</i>. The antimicrobial activity properties have been found to be due to the aldehyde group and the mannan -like binding properties from the adduct acting as a glycoprotein.&picture=http://www.aascit.org/aascit/decorators/img/journal/890.jpg)
| [1] | Joseph Torshian Ugye, Department of Chemical Sciences, Federal University, Wukari, Nigeria. |
| [2] | Adamu Uzairu, Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria. |
| [3] | Sulaiman Ola Idris, Department of Chemistry, Ahmadu Bello University, Zaria, Nigeria. |
| [4] | Helen Ochuko Kwanashie, Department of Pharmacology and Therapeutics, Ahmadu Bello University, Zaria, Nigeria. |
Formaldehyde and proteins cross link adducts have variously been reported and also associated with some health risk factors like diabetes. In this study, an 3,5-diphenylamine-1,4 dihydro-2,6-dimethylpyridine dicarboxylate adduct was prepared from a mixture of Formaldehyde, Acetoacetinalide and Plasma Albumin and was characterized using melting point, IR, Uv spectra characteristics as well as the antimicrobial activity based on the method described by Hantzsch. The adduct was found to have a melting temperature range of (179.5 - 180.4°C). The result of the FTIR analysis showed the functional groups present in the adduct as follows: The band at 2948.85 cm-1 was assigned to aldehyde (HC=O) group. The ones at 3850.5 cm-1 3736.73 and 3617.47 were found to be (O-H) stretch for water of hydration and N-H stretches. The sharp 2356.87 band was found to be due to N-H stretch from amines in the adduct while the bands at 1739.68, 1547.01 cm-1 and 1032.63 cm-1 were attributed to C=O and C-H stretch as well as C-N stretch due to tertiary amines respectively. The Antimicrobial screening test on the adduct also showed some antimicrobial effect to some micro- organism such as Bacillus sustilis (12.0 mm), Proteum mirabilis (16.0 mm) and Canidad albicans (14.0 mm) as their growth was inhibited when placed around their growth media. It was however found to have no effect on some fungi and algae such as Escherichia coli and Staphylococcus aureus. The antimicrobial activity properties have been found to be due to the aldehyde group and the mannan -like binding properties from the adduct acting as a glycoprotein.
Keywords
Mechanism, Biochemical Activity, Plasma Albumin, Formaldehyde, Glycoprotein
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