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International Journal of Chemical and Biomedical Science

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The Construction of a Novel Fusion Protein GSH-bFGF and the Characterization of Its Activities in vitro

International Journal of Chemical and Biomedical Science
Vol.2 , No. 2, Publication Date: Aug. 18, 2016, Page: 9-16

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Authors

[1]	Lin Chen, Department of Biology, School of Life Science and Technology, Jinan University, Guangzhou, China.
[2]	Yi Zou, Department of Biology, School of Life Science and Technology, Jinan University, Guangzhou, China.
[3]	Tianhong Zhou, Department of Biology, School of Life Science and Technology, Jinan University, Guangzhou, China.
[4]	Hongjian Li, Department of Biology, School of Life Science and Technology, Jinan University, Guangzhou, China.
[5]	Lin Cao, Department of Anesthesiology of Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, China.

Abstract

The basic fibroblast growth factor (bFGF) and glutathione (GSH) have extensive biological functions. The bFGF is currently used to treat cardiovascular diseases, osteoarthritis, chronic kidney disease, Parkinson’s disease and mood disorders due to its mitogenic activity. Also, GSH deficiency has been linked to a number of human diseases such as liver cirrhosis, pancreatic inflammation, diabetes, neurodegenerative diseases, pulmonary diseases and aging. In this study, we construct a novel fusion protein, glutathione-basic fibroblast growth factor (GSH-bFGF) and characterized its bio-activities in vitro. The results showed that GSH-bFGF promoted the proliferation of cultured NIH/3T3 and primary human umbilical vein endothelial cells (HUVECs) using CCK-8 assays. The fusion protein also displayed well preserved antioxidant activity using Total Antioxidant Capacity Assay and significantly inhibited the senescence of HUVECs. We therefore, proposed that comparing with GSH or bFGF alone, the synthetic novel fusion protein GSH-bFGF was likely to have improved potentials in treating aging related diseases, such as atherosclerosis and neurodegenerative diseases.

Keywords

GSH-bFGF, Proliferation, Antioxidant Ability, Cell Senescence, Atherosclerosis

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