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Vol.1 , No. 5, Publication Date: Dec. 11, 2015, Page: 119-129
 complex 1, [Cu<sub>2</sub> (glygly) <sub>2</sub> (ppz)(H<sub>2</sub>O) <sub>4</sub>].2H<sub>2</sub>O, derived from dipeptide (glycyl glycine anion) and piperazine as a metallopeptide drug with tRNA was examined by UV–visible, Fourier transform–infrared (FT–IR), and fluorescence spectroscopy, circular dichroism (CD), and isothermal titration calorimetry (ITC). The binding constants (K<sub>b</sub>), and binding site number (n), and corresponding thermodynamic parameters; ∆G, ∆H and ∆S were calculated. ITC results showed that the complex 1 binds strongly with tRNA (K<sub>b</sub> ~ 4.504 ×10<sup>4</sup>M<sup>–1</sup>). The distance between tRNA (donor) and complex 1 (acceptor) was estimated by means of fluorescence resonance energy transfer (FRET). The molecular docking technique utilized for ascertaining the mechanism and mode of action towards the molecular target tRNA indicated that complex 1 has a strong binding affinity to tRNA. Moreover, the growth inhibitory effects of the complex 1 on the sw480 cancer cell line were measured (IC<sub>50</sub> ∼ 2.87 μg/ml).&picture=http://www.aascit.org/aascit/decorators/img/journal/890.jpg)
| [1] | Waddhaah M. Al–Asbahy, Department of Chemistry, Taiz University, Taiz, Yemen. |
| [2] | Manal Shamsi, Department of Chemistry, Taiz University, Taiz, Yemen. |
| [3] | Niyazi A. S. Al–Areqi, Department of Chemistry, Taiz University, Taiz, Yemen. |
| [4] | Gamal Al–amery, Department of Microbology, Taiz University, Taiz, Yemen. |
Interaction of new dinuclear copper (II) complex 1, [Cu2 (glygly) 2 (ppz)(H2O) 4].2H2O, derived from dipeptide (glycyl glycine anion) and piperazine as a metallopeptide drug with tRNA was examined by UV–visible, Fourier transform–infrared (FT–IR), and fluorescence spectroscopy, circular dichroism (CD), and isothermal titration calorimetry (ITC). The binding constants (Kb), and binding site number (n), and corresponding thermodynamic parameters; ∆G, ∆H and ∆S were calculated. ITC results showed that the complex 1 binds strongly with tRNA (Kb ~ 4.504 ×104M–1). The distance between tRNA (donor) and complex 1 (acceptor) was estimated by means of fluorescence resonance energy transfer (FRET). The molecular docking technique utilized for ascertaining the mechanism and mode of action towards the molecular target tRNA indicated that complex 1 has a strong binding affinity to tRNA. Moreover, the growth inhibitory effects of the complex 1 on the sw480 cancer cell line were measured (IC50 ∼ 2.87 μg/ml).
Keywords
Transfer RNA, In vitro tRNA Binding, Circular Dichroism, Thermal Denaturation, Fluorescence Spectroscopy
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