American Journal of Food, Nutrition and Health  
Manuscript Information
 
 
Preparation of Novel Antimicrobial Meat Packaging Using Chitosan-Arginine
American Journal of Food, Nutrition and Health
Vol.1 , No. 2, Publication Date: May 25, 2016, Page: 7-12
2700 Views Since May 25, 2016, 962 Downloads Since May 25, 2016
 
 
Authors
 
[1]    

R. A. Lahmera, Department of Food Science and Technology, Faculty of Agriculture, University of Tripoli. Tripoli, Libya.

[2]    

A. P. Williamsb, School of Environment, Natural Resources & Geography, College of Natural Sciences, Bangor University, Bangor, LL57 2UW, UK.

[3]    

S. Townsendc, Synedgen, Inc., 1420 N Claremont Blvd Suite 105D, Claremont, CA 91711, USA.

[4]    

S. Bakerc, Synedgen, Inc., 1420 N Claremont Blvd Suite 105D, Claremont, CA 91711, USA.

[5]    

D. L. Jonesb, School of Environment, Natural Resources & Geography, College of Natural Sciences, Bangor University, Bangor, LL57 2UW, UK.

 
Abstract
 

Chitosan-arginine (Ch-arg) has been proposed as an anti-microbial agent to reduce the proliferation of spoilage and pathogenic bacteria within meat products destined for human consumption. In the current experiment its use as an antimicrobial packaging material was examined. Two different concentrations of chitosan-arginine (0.05 and 0.15% w/w) were blended into a cellulose film (Ch-arg film). When placed in contact with chicken and beef juice inoculated with a lux-marked strain of E. coli O157, the film incorporating the highest Ch-arg concentration resulted in a small reduction of E. coli O157 in chicken juice; however, there was no effect of the Ch-arg film on E. coli O157 in beef juice. The lack of observed effect in the beef juice experiment we ascribe to insufficient surface-to-surface contact between the film and the bacteria in the beef juice and the greater presence of other Ch-arg reactive components in the juice (e.g. fats, blood cells). Results suggest that, in combination with other antimicrobials, Ch-arg packaging may offers some potential for limiting the growth of pathogenic bacteria in foodstuffs; however, further research is needed to enhance their anti-microbial performance.


Keywords
 

Cross-Contamination, foodborne Pathogen, Polymer Film, Shelf Life


Reference
 
[01]    

Dutta, J., Tripathi, S., Dutta, P. K., 2012. Progress in antimicrobial activities of chitin, chitosan and its oligosaccharides: a systematic study needs for food applications. Food Science and Technology International 18, 3–34.

[02]    

Restuccia, D., Spizzirri, U. G., Parisi, O. I., Cirillo, G., Curcio, M., Iemma, F., Puoci, F., Vinci, G., Picci, N., 2010. New EU regulation aspects and global market of active and intelligent packaging for food industry applications. Food Control 21, 1425–1435.

[03]    

Bastarrachea, L., Dhawan, S., Sablani, S. S., 2011. Engineering properties of polymeric-based antimicrobial films for food packaging. Food Engineering Reviews 3, 79–93.

[04]    

Foster, L. J., Butt. J., 2011. Chitosan films are not antimicrobial. Biotechnology Letters 33, 417–421.

[05]    

Lee, C. H., An, D. S., Park, H. J., Lee, D. S., 2003. Wide-spectrum antimicrobial packaging materials incorporating nisin and chitosan in the coating. Packaging Technology and Science 16, 99-106.

[06]    

Alishahi, A., Aider, M., 2012. Applications of chitosan in the seafood industry and aquaculture: a Review. Food and Bioprocess Technology 3, 817–830.

[07]    

Lahmer, R. A., Williams, A. P., Townsend, S., Baker, S., Jones, D. L., 2012. Antibacterial action of chitosan-arginine against Escherichia coli O157 in chicken juice. Food Control 26, 206-211.

[08]    

Dutta, P. K., Tripathi, S., Mehrotra, G. K., Dutta, J., 2009. Perspectives for chitosan based antimicrobial films in food applications. Food Chemistry 114, 1173–1182.

[09]    

Soon, J. M., Chadd, S. A., Baines, R. N., 2011. Escherichia coli O157:H7 in beef cattle: on farm contamination and pre-slaughter control methods. Animal Health Research Reviews 12, 197–211.

[10]    

Pennington, H., 2010. Escherichia coli O157. The Lancet 376, 1428–1435.

[11]    

Mattick, K., Durham, K., Dommingue, G., Jørgensen, F., Sen, M., Schaffner, D, W., Humphrey, T., 2003. The survival of foodbone pathogens during domestic washing-up and subseguent transfer onto washing –up sponges, kitchen surfaces and food. International Journal of Food Microbiology 85, 213–226.

[12]    

Tang, H., Zhang, P., Kieft, T. L., Ryan, S. J., Baker, S. M., Wiesmann, W. P., Rogelj, S., 2010. Antibacterial action of a novel functionalized chitosan-arginine against Gram-negative bacteria. Acta Biomaterialia 6, 2562–2571.

[13]    

Gindl, W., Keckes, J., 2007. Drawing of self-reinforced cellulose films. Journal of Applied Polymer Science 103, 2703–2708.

[14]    

He, L. H, Xue R, Yang, D. B., Liu, Y., Song R., 2009. Effects of blending chitosan with peg on surface morphology, crystallization and thermal properties. Chinese Journal of Polymer Science, 27, 501–510.

[15]    

Li, Z., Zhuang, X. P., Liu, X. F., Guan, Y. L., De Yao, K., 2002. Study on antibacterial O-carboxymethylated chitosan/cellulose blend film from LiCl/N, N-dimethylacetamide solution. Polymer 43, 1541–1547.

[16]    

Sangsuwan, J., Rattanapanone, N., Rachtanapun, P., 2008. Effect of chitosan/methyl cellulose films on microbial and quality characteristics of fresh-cut cantaloupe and pineapple. Postharvest Biology and Technology 49, 403–410.

[17]    

Ritchie, J. M., Campbell, G. R., Shepherd, J., Beaton, Y., Jones, D., Killham, K., Artz, R. R. E., 2003. A stable bioluminescent construct of Escherichia coli O157:H7 for hazard assessments of long-term survival in the environment. Applied and Environmental Microbiology 69, 3359–3367.

[18]    

Williams, A. P., McGregor, K. A., Killham, K., Jones, D. L., 2008. Persistence and metabolic activity of Escherichia coli O157:H7 in farm animal faeces. FEMS Microbiology Letters 287, 168–173.

[19]    

Avery, L. M., Killham, K., Jones, D. L., 2005. Survival of E. coli O157:H7 in organic wastes destined for land application. Journal of Applied Microbiology 98, 814–822.

[20]    

Mauriello, G., DeLuca, E., La Storia, A., Villani, F, Ercolini, D., 2005. Antimicrobial activity of a nisin-activated p; ,^#astic film for food packaging. Letters Applied Microbiology 41, 464-469.

[21]    

Molecular Probes, 2004. Live/Dead® BacLightTM Bacterial Viability Kits http://www.probes.invitrogen.com/media/pis/mp07007.pdf (accessed 5/10/12)

[22]    

Xiao, B., Wan, Y., Zhao, M., Liu, Y., Zhang, S., 2011. Preparation and characterization of antimicrobial chitosan-N-arginine with different degrees of substitution. Carbohydrate Polymers 83, 144–150.

[23]    

Wu, Y., Yu, S., Mi, F., Wu, C., Shyu, S., Peng, C., Chao, A., 2004. Preparation and characterization on mechanical and antibacterial properties of chitsoan/cellulose blends. Carbohydrate Polymers 57, 435–440.

[24]    

Devlieghere, F., Vermeulen, A., Debevere, J., 2004. Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetable. Food Microbiology 21, 703–714.





 
  Join Us
 
  Join as Reviewer
 
  Join Editorial Board
 
share:
 
 
Submission
 
 
Membership