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Vol.2 , No. 5, Publication Date: Oct. 17, 2017, Page: 34-43
 can be mediated with increase in abscisic acid (ABA) level. Treatment of plants with exogenous ABA, therefore, induces responses of stress-associated genes. The objective of this study was to characterize Arabidopsis thaliana stress-associated genes for their role in DT in germinated A. thaliana seeds. Four different mutant lines of Arabidopsis (sod1, daa1, di19 and vip1) were used, which stress-associated genes are knocked out. Seeds of these mutants were selected at two stages (RP-Radical Protrusion and RH-Root Hair), and analyzed for ability to acquire DT in mild-osmotic stress by applying PEG. Levels of gene expression were analyzed using RT-qPCR at RP and RH before and after PEG treatment. Eight key genes of the ABA response pathway were selected and checked for their expression level. Quantitative gene expression data for RP and RH stages showed an overall trend to increase the expression level after PEG treatment. Expression of genes of ABA biosynthesis and the receptors increase in response to PEG treatment, while ABA catabolic gene decreases. The author conclude that ABA genes are assumed to be involved in response to DT, although, three mutants: sod1, daa1 and di19 showed an increase of 20% in acquisition of DT compared to the wild type, genes involved in ABA pathway did not show difference related to phenotype.&picture=http://www.aascit.org/aascit/decorators/img/journal/805.jpg)
| [1] | Adane Gebeyehu, National Agricultural Biotechnology Research Center, Ethiopia. |
The present study was conducted to characterize the role of Arabidopsis thaliana genes in desiccation tolerance during seed to seedling transition. Dehydration stress, such as drought and desiccation, affects the physiological and biochemical activities of seeds and seedlings through either suppressing or inducing stress responsive genes of plants. Responsiveness of genes related to desiccation tolerance (DT) can be mediated with increase in abscisic acid (ABA) level. Treatment of plants with exogenous ABA, therefore, induces responses of stress-associated genes. The objective of this study was to characterize Arabidopsis thaliana stress-associated genes for their role in DT in germinated A. thaliana seeds. Four different mutant lines of Arabidopsis (sod1, daa1, di19 and vip1) were used, which stress-associated genes are knocked out. Seeds of these mutants were selected at two stages (RP-Radical Protrusion and RH-Root Hair), and analyzed for ability to acquire DT in mild-osmotic stress by applying PEG. Levels of gene expression were analyzed using RT-qPCR at RP and RH before and after PEG treatment. Eight key genes of the ABA response pathway were selected and checked for their expression level. Quantitative gene expression data for RP and RH stages showed an overall trend to increase the expression level after PEG treatment. Expression of genes of ABA biosynthesis and the receptors increase in response to PEG treatment, while ABA catabolic gene decreases. The author conclude that ABA genes are assumed to be involved in response to DT, although, three mutants: sod1, daa1 and di19 showed an increase of 20% in acquisition of DT compared to the wild type, genes involved in ABA pathway did not show difference related to phenotype.
Keywords
Dehydration, Radical Protrusion, Root Hair
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