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Vol.2 , No. 1, Publication Date: Aug. 25, 2017, Page: 1-12
 were tested for suitability for mass production of <i>M. anisopliae</i> and B. <i>bassiana</i>. High conidial yields of more than 10<sup>10</sup> conidia ml<sup>-1</sup> for <i>M. anisopliae</i> and around 4.0 x 10<sup>9</sup> conidia ml<sup>-1</sup> for <i>B. bassiana</i> were harvested from spoiled cereals. 2.0 - 4.0 x 10<sup>9</sup> conidia ml<sup>-1</sup> for both spp. was collected from potato and carrot peel substrates. However, olive pomace (jifett), sawdust and orange peel substrates were found unsuitable for mass production. Carbon and nitrogen content of resultant conidia were affected by the substrate. The lowest C:N value of 4 was estimated for conidia of <i>B. bassiana</i> cultured on rice and the highest of 7.9 for <i>M. anisopliae</i> cultured on SDA. Conidial germination was exceeding 95% for all cultures. Total control of <i>T. absoluta</i> eggs was achieved under laboratory conditions using conidia from rice and burghul. Conidia of <i>M. anisopliae</i> from the same substrates caused 80% of egg mortalities under the same conditions. Good control of eggs and larvae in a greenhouse were observed after 3<sup>rd</sup> application of <i>B. bassiana</i> cultured on potato peels suggesting that this substrate is a potential candidate for sustainable mass production of EPF and their use in IPM strategies in the greenhouses.&picture=http://www.aascit.org/aascit/decorators/img/journal/805.jpg)
| [1] | Ludmilla Ibrahim, Department of Crop Production, Faculty of Agriculture and Veterinary Sciences, Lebanese University, Beirut, Lebanon. |
| [2] | Cynthia Dakache, Department of Crop Production, Faculty of Agriculture and Veterinary Sciences, Lebanese University, Beirut, Lebanon. |
| [3] | Mounira El Kreidy, Department of Crop Production, Faculty of Agriculture and Veterinary Sciences, Lebanese University, Beirut, Lebanon. |
| [4] | Rasha Dagher, Department of Crop Production, Faculty of Agriculture and Veterinary Sciences, Lebanese University, Beirut, Lebanon. |
| [5] | Nour Ezzeddine, Department of Crop Production, Faculty of Agriculture and Veterinary Sciences, Lebanese University, Beirut, Lebanon. |
| [6] | Said Ibrahim, Department of Crop Production, Faculty of Agriculture and Veterinary Sciences, Lebanese University, Beirut, Lebanon. |
Extensive insecticide use can cause resistance in many insect-pests and also undesired side-effects on human and environment. Leaf miner, Tuta absoluta, is a new generation of devastating pests that rapidly spreading throughout the world causing up to 100% of tomato yield losses. In this view, it is a necessary consequence to apply environmentally-friendly strategies in order to control this pest effectively. As a result, research studies were undertaken in hope to find some waste materials that could be successfully used for sustainable mass-production of Lebanese isolates and their utilisation against T. absoluta. Eight different solid substrates (mostly waste materials) were tested for suitability for mass production of M. anisopliae and B. bassiana. High conidial yields of more than 1010 conidia ml-1 for M. anisopliae and around 4.0 x 109 conidia ml-1 for B. bassiana were harvested from spoiled cereals. 2.0 - 4.0 x 109 conidia ml-1 for both spp. was collected from potato and carrot peel substrates. However, olive pomace (jifett), sawdust and orange peel substrates were found unsuitable for mass production. Carbon and nitrogen content of resultant conidia were affected by the substrate. The lowest C:N value of 4 was estimated for conidia of B. bassiana cultured on rice and the highest of 7.9 for M. anisopliae cultured on SDA. Conidial germination was exceeding 95% for all cultures. Total control of T. absoluta eggs was achieved under laboratory conditions using conidia from rice and burghul. Conidia of M. anisopliae from the same substrates caused 80% of egg mortalities under the same conditions. Good control of eggs and larvae in a greenhouse were observed after 3rd application of B. bassiana cultured on potato peels suggesting that this substrate is a potential candidate for sustainable mass production of EPF and their use in IPM strategies in the greenhouses.
Keywords
Recycled Materials, Mass Production, B. bassiana, M. anisopliae, T. absoluta, Biological Control
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