International Journal of Biological and Environmental Engineering  
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Co-digestion of Plant Residuals and Chicken Dung in Two-Stage Solid State Anaerobic System with Single and Multiple Hydrolysers
International Journal of Biological and Environmental Engineering
Vol.2 , No. 1, Publication Date: Aug. 8, 2019, Page: 1-8
1631 Views Since August 8, 2019, 526 Downloads Since Aug. 8, 2019

Natalya Akinshina, Department of Applied Ecology and Sustainable Development, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan.


Azamat Azizov, Department of Applied Ecology and Sustainable Development, National University of Uzbekistan Named After Mirzo Ulugbek, Tashkent, Uzbekistan.


Solid-state anaerobic digestion has been widely used to treat various types of organic wastes. Generally, hydrolysis is considered as a rate-limiting step of anaerobic digestion. It tends to produce the higher concentration volatile fatty acids under a high organic loading rate. But, if the concentrations of fatty acids in the single digester are too high anaerobic digestion could be inhibited or failed. That’s why division of whole digestion process into 2 stages (Hydrolysis and Methanogenesis) was developed, it makes possible to support high level of methane-production at the high loading rate. In 2-stage anaerobic system different process conditions (such as pH value or temperature) could be adjusted and optimized for the individual groups of microbes in certain degradation steps. Presented paper describes methane production in two-stage solid state anaerobic digestion of plant residuals and chicken dung using lab-scale system. The main objectives of the study were: (1) to examine the influence of temperature regime of hydrolysis stage on whole methane production; (2) to evaluate methane yield in anaerobic digestion of fruit/vegetable waste or hay biomass with a chicken dung; (3) to assess contribution of different stages the digestion into cumulative methane production. All substrates were air dried (at 25-27°C) and well grinded before experiments. Methanogenesis reactors were operated under mesophilic conditions; hydrolysis stages were under mesophilic or thermophilic temperature. Methane production rate and cumulative methane yield was measured separately in hydrolysis and methanogenesis reactors. Total digestion of the substrates at 55°C hydrolysis was in progress 15-17 days; at 37°C hydrolysis – 21-23 days. Methane yield in 2-stage solid state anaerobic digestion of dung/fruit-vegetables waste under 55°C-hydrolysis was 20% higher as against 37°C-hydrolysis (283.5 versus 236 ml CH4/gVS); the digestion of dung/hay mixture gave 29% higher methane gas under 55°C-hydrolysis (233 versus 180 ml CH4/gVS). It was revealed that the most of total methane gas in 2-stage SS-AD lab-system under ambient pressure was produced in the methanogenesis reactor. A wave-like methane production was observed in hydrolysis reactors, the maximum gas was released in the first day. Periodically, after each 5-7 days the production was stopped and liquid fraction of hydrolysate was withdrawn as a feedstock for methanogenesis reactor. This led to some interruption in the loading of methane-tank and considered as inconvenience for an operation under continuous mode. Integrating of multiple hydrolysis reactors into common system with strong order of loading and withdrawal provides more constant production of feedstock and maintains stable gas production. It is also suggested to accumulate hydrolisates of different withdrawals in one storage unit and mix them before dosed food supply into methanogenesis reactor.


Two-stage SS-AD, Co-fermentation, Plant Residuals, Chicken Dung, Methane Production


Third National Communication of the Republic of Uzbekistan on the UN Framework Convention on Climate Change, Tashkent, 2016.


G. Saidova, T. Salikhov, Kh. Kabulova, A. Elisov, “Alternative energy sources: Potential use in Uzbekistan”, Center for Economic Research, Analytical Report, Tashkent, 2011/03.


B. Rincón, R. Borja, J. M. González, M. C. Portillo, and C. Sáiz-Jiménez, “Influence of organic loading rate and hydraulic retention time on the performance, stability and microbial communities of one-stage anaerobic digestion of two-phase olive mill solid residue”, Biochem Eng J, vol. 40, pp. 253-261, 2008.


J. Jianguo, Z. Yujing, L. Kaimin, W. Quan, G. Changxiu, L. Menglu, “Volatile fatty acids production from food waste: Effects of pH, temperature, and organic loading rate”, Bioresour Technol, vol. 143, pp. 525-530, 2013.


P. Świątczak, A. Cydzik-Kwiatkowska, P. Rusanowska, “Microbiota of anaerobic digesters in a full-scale wastewater treatment plant”, J Arch Environ Protect, vol. 43 (3), pp. 53-60, 2017.


Y. B. Li, J. Y. Zhu, C. X. Wan, S. Y. Park, “Solid-state anaerobic digestion of corn stover for biogas production”, Trans ASABE, vol. 54, pp. 1415-21, 2011.


Y. B. Li, S. Y. Park, J. Y. Zhu, “Solid-state anaerobic digestion for methane production from organic waste”, Renew Sust Energy Rev, vol. 15, pp. 821-826, 2011.


D. Brown, J. Shi, Y. Li, “Comparison of solid-state to liquid anaerobic digestion of lignocellulosic feedstocks for biogas production”, Bioresour Technol, vol. 124, pp. 379-386, 2012.


E. Aymerich, M. Esteban-Gutiérrez, L. Sancho, “Analysis of the stability of high-solids anaerobic digestion of agro-industrial waste and sewage sludge”, Bioresour Technol, vol. 144, pp. 107-114, 2013.


J. A. Álvarez, L. Otero, J. M. Lema, “A methodology for optimizing feed composition for anaerobic co-digestion of agro-industrial wastes”, Bioresour Technol, vol. 101, pp. 1153-58, 2010.


H. Hartmann, H. B. Møller, B. K. Ahring, “Efficiency of the anaerobic treatment of the organic fraction of municipal solid waste: collection and pretreatment”, Waste Manage Res, vol. 22, pp. 35-41, 2004.


D. Brown, Y. B. Li, “Solid state anaerobic co-digestion of yard waste and food waste for biogas production”, Bioresour Technol, vol. 127, pp. 275-280, 2013.


J. C. Motte, R. Escudié, N. Bernet, J. P. Delgenes, J. P. Steyer, C. Dumas, “Dynamic effect of TS content, low substrate/inoculum ratio and particle size on solid-state anaerobic digestion”, Bioresour Technol, vol. 144, pp. 141-148, 2013.


K. Bär, W. Merkle, M. Tuczinski, F. Saravia, H. Horn, F. Ortloff, F. Graf, A. Lemmer, T. Kolb, “Development of an innovative two-stage fermentation process for high-calorific biogas at elevated pressure”, Biomass Bioenerg, vol. 115, pp. 186–194, 2018.


K. C. Wijekoon, C. Visvanathan, A. Abeynayaka, “Effect of organic loading rate on VFA production, organic matter removal and microbial activity of at two-stage thermophilic anaerobic membrane bioreactor”, Bioresour Technol, vol. 102 (9), pp. 5353-60, 2011.


S-M. Lee, J-Y. Jung, Y-C. Chung, “Novel method for enhancing permeates flux of submerged membrane system in two-phase anaerobic reactor”, Water Res, vol. 35 (2), pp. 471–477, 2001.


S. Zielonka, A. Lemmer, H. Oechsner, T. Jungbluth, “Energy balance of a two-phase anaerobic process for energy crops”, Eng Life Sci, vol. 10 (6), pp. 515–519, 2010.


A. Lemmer, Y. Chen, J. Lindner, A. M. Wonneberger, S. Zielonka, H. Oechsner, T. Jungbluth, “Influence of different substrates on the performance of a two-stage high pressure anaerobic digestion system”, Bioressour Technol, vol. 178, pp. 313–318, 2015.


J. Lindner, S. Zielonka, H. Oechsner, A. Lemmer, “Is the continuous two-stage anaerobic digestion process well suited for all substrates?”, Bioresour Technol, vol. 200, pp. 470–476, 2016.


W. Merkle, K. Baer, J. Lindner, S. Zielonka, F. Ortloff, F. Graf, T. Kolb, T. Jungbluth, A. Lemmer, “Influence of pressures up to 50 bar on two-stage anaerobic digestion”, Bioresour Technol, vol. 232, pp. 72–78, 2017.


APHA, Standard Methods for the Examination of Water and Wastewater, 20th ed. American Public Health Association. Washington DC, USA, 1999.


Application Bulletin No. 133/2 e. Determination of ammonium with the ion-selective electrode. Metrohm.


C. A. L. Chernicharo, Biological Wastewater Treatment Series. Volume 4. Anaerobic Reactors. IWA Publishing. London - New York, 2007.

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